Increased in-vitro and in-vivo biological activity of lipopolysaccharide extracted from clinical low virulence vacA genotype Helicobacter pylori strains

Salgado, Fernando; García, Antonio Manuel Rodríguez; Oñate, Ángel; González, Carlos; Kawaguchi, Fernando Aparecido

doi:10.1099/0022-1317-51-9-771

Cited by 10 publications

(6 citation statements)

References 29 publications

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“…Schmidt et al (10) reported that the cagA and vacAs2/m2 positive H. pylori strains induced low levels of IL-8 secretion in AGS cells. The studies performed by Salgado et al (40) indicated that the cagA and vacAs2/m2 positive H. pylori strains caused an increase in TNF-α levels of mice spleen cells. Andres et al (41) reported that H. pylori defines the local immune response in susceptible hosts by interacting with dendritic cells in a strain-specific state, and this interaction can lead to either mild infection or to the disease development.…”

Section: Discussionmentioning

confidence: 99%

The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

Caliskan

Sayi-Yazgan²,

Tokman³

et al. 2015

Turk J Gastroenterol

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Background/Aims: Helicobacter pylori (H. pylori) is a microaerophilic bacterium related with peptic ulcer and gastric cancer. Its virulence factors include cytotoxin-associated gene A (CagA) and vacuolating cytotoxin gene A (VacA) proteins. Cytokine release inducted by H. pylori colonization has an important role in pathogenesis of H. pylori. The severity of gastric pathologies depends on the H. pylori genotypes found in different geographical regions. We aimed to determine the relationship between different H. pylori genotypes and their effects on the cytokine release levels. Materials and Methods: ureC, cagA, vacAs1/s2, vacAm1/m2, and blood group antigen-binding adhesion protein A2 (babA2) virulence related genes were investigated in 21 H. pylori strains. Genotyping of 21 strains were made due to the presence of cagA, vacAs1/s2, vacAm1/m2, and babA2 genes. The H. pylori strains were cultured together with THP-1 and neutrophil-differentiated Human promyelocytic leukemia cells (HL-60) cells. The levels of cytokines interleukin (IL)-1β, IL-6, IL-8, IL-12, tumor necrosis factor-alpha (TNF-α), and IL-10 in these cells were measured after co-culturing with H. pylori strains. Results: The following five different genotypes were detected: Genotype1: cagA and vacAs1m2; Genotype2: cagA and vacAs1m1; Genotype3: cagA, vacAs1m2, and babA2; Genotype4: vacAs2m2; and Genotype5: cagA and vacAs2m2. All these genotypes significantly induced the levels of IL-1β, IL-6, IL-8, IL 10, and TNF-α in THP-1 cells. Genotype 5 caused higher amounts of IL-1β, IL-6, TNF-α, and IL-10, whereas genotype 1 induced the highest levels of IL-8. In neutrophil-differentiated HL-60 cells, genotype 4 increased IL-6 levels and genotype 3 and 4 elevated IL-8 levels significantly. Conclusion: These results suggested that cytokine response of the host varies depending on the specific immune response of the host against different H. pylori strains.

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Section: Discussionmentioning

confidence: 99%

The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

Caliskan

Sayi-Yazgan²,

Tokman³

et al. 2015

Turk J Gastroenterol

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“…Nuestro grupo de investigación está abocado a caracterizar cepas de H pylori que infectan a pacientes locales, en base a genes de virulencia de importancia reconocida por su asociación con patologías gastroduodenales severas 23,29 . También contamos con información patofisiológica útil, que puede tener implicancias clínicas significativas 30 , pues estudios de poblaciones de oriente y occidente señalan que los factores de virulencia de H pylori varían de acuerdo a la localidad geográfica y a la edad del individuo 31 .…”

Section: Discussionunclassified

Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos

C¹,

T²,

Sch³

et al. 2006

Rev. méd. Chile

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“…In one study LPS from type I strains evokes distinct toll-like receptor 4 signalling and may help to induce apoptosis (Kawahara et al, 2001a). Another study has shown that LPS, which is relatively more immunologically active, is expressed by strains with less toxic vacA strains with the allelic variants s1bm2 or s2m2 (Salgado et al, 2002). A detailed study of strains from the European population indicated that the strain type associated with atrophic…”

Section: Interrelationship Of Toxin Expression For H Pylorimentioning

confidence: 96%

Toxins of the Helicobacter Genus and Their Roles in Pathogenesis

Hynes

Wadström

2004

Journal of Toxicology: Toxin Reviews

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Increased in-vitro and in-vivo biological activity of lipopolysaccharide extracted from clinical low virulence vacA genotype Helicobacter pylori strains

Cited by 10 publications

References 29 publications

The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

The cytokine response in THP-1 (monocyte) and HL-60 (neutrophil-differentiated) cells infected with different genotypes of Helicobacter pylori strains

Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos

Toxins of the Helicobacter Genus and Their Roles in Pathogenesis

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