Increased osteogenic differentiation potential of MSCs cultured on nanofibrous structure through activation of Wnt/β-catenin signalling by inorganic polyphosphate

Abstract

There are several signal pathways involved in bone regeneration that can be triggered by several factors including mechanical, physical and chemical factors. Chemical growth factors are commonly used in differentiation induction to stem cells, while have side effects. In this study, inorganic poly-phosphate (poly-P) as a natural-based molecule was used to induce Wnt/b-catenin signalling in adiposederived mesenchymal stem cells (AT-MSCs) while cultured on 2D and 3D culture systems. Common osteogenic markers were investigated to detect the influence of Wnt/b-catenin signalling induction on osteogenic differentiation of AT-MSCs and signalling genes up regulation was also evaluated by its related gene expression measurement. Results were shown that Cyclin-D1 and b-catenin gene expression was significantly increased in those cells treated by poly-P. Osteogenic differentiation of those stem cells with higher Cyclin-D1 and b-catenin gene expression was significantly higher than other groups except those stem cells cultured under osteogenic medium. According to the results, inorganic poly-P can trigger osteogenic differentiation in stem cells through Wnt/b-catenin signalling and this potential is almost close to common osteogenic growth factors and this could be used as naturalbased molecules in bone regeneration, apart from concerns about the use of chemical factors.

Nanotechnology-based products for cancer immunotherapy

“… In the fourth strategy, the nanofibrous scaffold can be applied as a delivery tool for certain biological factors. This strategy provides a combination biologic therapy plan contain concurrent delivery of genes, cells, and other drugs [ 12 , 14 ]. …”

Plastics derived endocrine‐disrupting compounds and their effects on early development

“…In animal models, phthalate exposure downregulates both Thrα1 and Thrβ1 genes and inhibits nuclear translocation in the placenta (Yu et al, 2018). Di‐(2‐ethylhexyl) phthalate was reported to disrupt homeostasis of thyroid function (Ardeshirylajimi, Golchin, Khojasteh, & Bandehpour, 2018) and thyroid hormones (THRs) in pregnant women and fetuses, and affect placental TH transport (Du et al, 2020). Furthermore, exposure to PBDEs modifies the placental content of IGF‐1 (Xu et al, 2013).…”

Poly-phosphate increases SMC differentiation of mesenchymal stem cells on PLGA–polyurethane nanofibrous scaffold

“…Adenylate kinase, used as a phosphate donor for some glucose kinases, or may play a role in apoptosis or response of cells to osmotic pressure and pH pressure. Recent studies have shown also the role of poly-P in bone formation, cell proliferation, apoptosis, metastatic tumors, blood clots, and the regulation of inflammation (Ardeshirylajimi et al 2018;Dedkova and Blatter 2014;Solesio et al 2016). These poly-P roles are mediated by stimulation of intracellular signaling pathways.…”