Increased osteonectin expression is associated with malignant transformation and tumor associated fibrosis in the lung

Molecular signatures of metaplastic carcinoma of the breast by large-scale transcriptional profiling: identification of genes potentially related to epithelial–mesenchymal transition

“…The immunohistochemical demonstration of the intense expression of SPARC in sarcomatous tumor cells strongly suggests a potential role for SPARC in EMT. This notion is further supported in vitro by its deadhesive and motility-increasing effects on culture cells (Framson and Sage, 2004) and in vivo by its expression in pulmonary sarcomatoid carcinoma tumor cells, but not those of conventional lung cancer (Siddiq et al, 2004). In line with the critical role of EMT in cancer invasion and metastasis (Thiery, 2002), we found the expression of SPARC and vimentin to be independently associated with poor overall survival in breast cancer patients, findings also confirmed by other groups (Jones et al, 2004;Korsching et al, 2005).…”

Maspin nuclear localization is linked to favorable morphological features in pulmonary adenocarcinoma

“…Normal immortalized bronchial epithelial cells BEAS 2B (gift from John Lechner, NIH) were transformed by culture in the presence of cigarette smoke concentrate (CSC, gift of John Lechner) at a final concentration of 5 g/ml, deriving three clonal cell lines, designated clones 1-3, as previously detailed [35], corresponding respectively to clones designated CSC 2-4 in the current study. Parental BEAS 2B cells were cultured in LHC 8 media (Rockville Biofluids, MD), while the CSC-transformed clonal cells were cultured in LHC/L15 (1:1) supplemented with 10% FCS.…”

“…To initially investigate the involvement of maspin in lung carcinogenesis, we analyzed the expression of maspin at the protein level in a previously characterized in vitro system of chemical transformation [32][33][34][35]. As reported earlier, CSC-treatment of immortalized non-transformed BEAS 2B cells resulted in clonal cell lines CSC 2-4 (previously designated clones 1-3) that showed 9-13-fold increase of anchorage-independent colony forming efficiency compared to the parental vehicletreated or untreated cell line [35].…”

“…In order to address these questions, we studied how cell transformation affects maspin expression in vitro, using a previously described model of bronchial epithelial cell carcinogenesis [32][33][34][35]. We also compared the expression pattern of maspin in normal lung and resected NSCLC by immunohistochemistry.…”

Prognostic Value of SPARC Expression in Unresectable NSCLC Treated with Concurrent Chemoradiotherapy

“…In previous studies, it was failed to demonstrate SPARC expression in normal lung tissue other than focal and weak staining at vessel walls and alveolar septa (Siddiq et al, 2004). In contrast, majority of tissues with NSCLC (75%) showed cytoplasmic SPARC expression.…”