Increased viability of fibroblasts when pretreated with ceria nanoparticles during serum deprivation

Abstract

Conditions of cellular stress are often the cause of cell death or dysfunction. Sustained cell stress can lead to several health complications, such as extensive inflammatory responses, tumor growth, and necrosis. To prevent disease and protect human tissue during these conditions and to avoid medication side effects, nanomaterials with unique characteristics have been applied to biological systems. This paper introduces the pretreatment in human dermal fibroblasts with cerium oxide nanoparticles during nutritional stress. For this purpose, human dermal fibroblast cells received cell culture media with concentrations of 250 µg/mL and 500 µg/mL of nano-cerium oxide before being exposed to 24, 48, and 72 hours of serum starvation. Contrast images demonstrated higher cell confluence and cell integrity in cells pretreated with ceria nanoparticles compared to untreated cells. It was confirmed by MTS assay after 72 hours of serum starvation that higher cell viability was achieved with ceria nanoparticles. The results demonstrate the potential of cerium oxide nanoparticles as protective agents during cellular starvation.

Cytocompatibility of Potential Bioactive Cerium-Doped Glasses based on 45S5

“…In particular, at the longest time (72 h), these two glasses show a cellular viability superior to the negative control. These findings agree with what Genier et al described: In fact, their results on cell viability (MTS assays) indicate that pre-treatment with nanoceria increased the cell population by 51.1% after starvation; this highlights how nanoceria seems to suppress cellular stress conditions by interfering with ROS generation [42]. This is fully in agreement with the previously-reported capacity of these glasses to mimic CAT and SOD enzymatic-activities [27].…”

P2O5-Free Cerium Containing Glasses: Bioactivity and Cytocompatibility Evaluation

“…The cell viability of K5.3 increases also with dilution (particularly 1:2 and 1:5) after 24 h: this shows that the low-concentration of eluate from K5.3 has already a good effect on cellular vitality. These results show that the addition of cerium has a positive effect on the extra and intracellular environment that interacts with cellular stress defense mechanisms [48,49].…”

Ceria Nanoparticles Decrease UVA-Induced Fibroblast Death Through Cell Redox Regulation Leading to Cell Survival, Migration and Proliferation

“…However, the surface chemistry dependent nature is well-represented though depends strongly, and in some cases dramatically, on particle valency, size, morphology, concentration, and exposure time (Gagnon and Fromm, 2015). The resulting cellular mechanism/response is less clear with activity attributed to activation of survival pathways such as stimulation of Bcl-2 expression and reduction of stress condition (Bizanek et al, 2018) or by nanoceria capacity in reducing apoptosis and inhibiting activation of MAP kinase pathway (Walkey et al, 2015). Interestingly, herein, we found that nanoceria increase cellular proliferation however decreased UVA-induced ERK activation.…”