Increases in callus formation and mechanical strength of healing fractures in old rats treated with parathyroid hormone

Andreassen, Troels T.; Fledelius, Christian; Ejersted, Charlotte; Oxlund, Hans

doi:10.1080/00016470152846673

Cited by 115 publications

(61 citation statements)

References 14 publications

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“…Decalcified tissues were embedded in paraffin. Specimens were cut transversally into 5 p thick sections and transferred to poly-lysinecoated slides for conventional hematoxylin+osin, alcian blue or alizarin red staining (Sigma Chemicals Inc, St. Louis, MO, USA) to distinguish fibrous, cartilaginous and bony tissues as previously described [1,2]. Five regions within the specimens from three sections obtained from each specimen were randomly selected ( Fig.…”

Section: Histomorphological Assessmentmentioning

confidence: 99%

“…Other studies have also shown that parathyroid hormone and physical factors such as CPM (continuous passive motion) could accelerate tissue healing of the joint including bone and cartilage [2,19,20]. The design of this study included an intra-articular tendon transplantation mimicking ACL reconstruction under physiological loading of the knee similar to that used in clinical application.…”

Section: Tensile Strength Testmentioning

confidence: 99%

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The effect of shock wave treatment at the tendon–bone interface—an histomorphological and biomechanical study in rabbits

Wang

Yang

et al. 2005

Journal Orthopaedic Research

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Purpose. This study was performed to investigate the effect of shock wave treatment on the healing at tendon-bone interface in rabbits.Materials and methods. Thirty-six New Zealand White rabbits were used in this study. The anterior cruciate ligament was excised and replaced with the long digital extensor. The right knees (study group) were treated with 500 impulses of shock waves at 14kV, while the left knees (control group) received no shock waves. Histomorphological studies were performed in 24 rabbits at I , 2,4, 8, 12 and 24 weeks. Biomechanical studies were performed in 12 rabbits at 12 and 24 weeks.Results. There was significantly more trabecular bone around the tendons noted in the study group compared with the control group at different time intervals after 4 weeks (P < 0.05). The contacting between bone and tendon was significantly better in the study group than the control group after 8 weeks (P < 0.05). The tensile strength of the tendon-bone interface was significantly higher in the study group than the control group at 24 weeks (P = 0.018), whereas similar modes of graft failure were noted between the two groups. Conclusion. Shock wave treatment significantly improves the healing rate of the tendon-bone interface in a bone tunnel in rabbits. The effect of shock waves appears to be time-dependent.

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Section: Histomorphological Assessmentmentioning

confidence: 99%

Section: Tensile Strength Testmentioning

confidence: 99%

The effect of shock wave treatment at the tendon–bone interface—an histomorphological and biomechanical study in rabbits

Wang

Yang

et al. 2005

Journal Orthopaedic Research

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“…Recent experiments have demonstrated that PTH(1-34) treatment is able to increase the meThe anabolic effect of intermittent parathyroid horchanical strength and callus formation in normal healing mone (PTH( 1-34)) administration on both cortical and fractures [1, 2,8]. Also in delayed fracture healing, cancellous bone has been established in a number of PTH(1-34) has been found to induce an anabolic effect animal studies [7].…”

Section: Introductionmentioning

confidence: 99%

Intermittent parathyroid hormone (1?34) enhances mechanical strength and density of new bone after distraction osteogenesis in rats

SEEBACH¹

2004

Journal of Orthopaedic Research

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Distraction osteogenesis is used both for leg lengthening and for bone transportation in the treatment of fractures and nonunions. The main problem with this method is that the time until full recovery may be up to a year, partly because of the time needed for the new formed bone to consolidate and become strong enough for weight bearing. We have studied whether intermittent parathyroid hormone (PTH( 1-34)) could accelerate the consolidation of new formed bone after distraction osteogenesis in rats.Forty-seven, 3-months-old male Sprague-Dawley rats underwent lengthening of the right femur using an external fixator. After a middiaphyseal osteotomy and a 7-day latency period, the callus was distracted during 10 days, with a distraction rate of 0.25 mm twice a day. The consolidation time was either 20 days or 40 days after distraction was completed. A dose of 60 pg of human PTH(I-34)/kg body weightlinjection or vehicle was given every second day beginning 30 days before the rats were killed. Both femura of each rat were subjected to mechanical testing and dual-energy X-ray absorptiometry. Blinded histological examination was done for the distracted femura.In the 20 days consolidation experiment, PTH( 1-34) increased ultimate load (56'%)), stiffness (1 17'%1), total regenerate callus volume (58'%1), callus BMC (24%) and histologic bone density (35%) compared to untreated distraction osteogenesis specimens. In the 40 days consolidation experiment, PTH( 1-34) increased ultimate load (54'%1), stiffness (55'%), callus BMC (33%) and histologic bone density (23%)) compared to untreated distraction osteogenesis specimens, Total regenerate callus volume was unchanged.The contralateral femur also became stronger, stiffer and denser under PTH( 1-34) treatment, but to a lesser degree. PTH(1-34) might become useful to shorten the consolidation time after distraction osteogenesis in humans.

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“…However, when rhPTH (1-34) is administered therapeutically, the oncedaily bolus administration creates a pulsatile exposure and leads to increased osteogenesis 34,35 . Studies in models of fracture-healing have demonstrated that rhPTH (1-34), the active fragment of this polypeptide, consistently enhances the stiffness and strength of experimental fractures during the healing process [36][37][38][39] . In an investigation by Alkhiary et al 38 the effects of rhPTH (1-34) (teriparatide) on fracture-healing was studied in 270 rats that had undergone standard closed femoral fractures and received doses of teriparatide that are similar to those that are effective in the treatment of osteoporosis in postmenopausal women.…”

Section: Current Status Of Osteoinductive Agents For Spine Fusionmentioning

confidence: 99%

2011 AOA Symposium: Tissue Engineering and Tissue Regeneration

Rodeo

Boden

Murray³

et al. 2013

The Journal of Bone and Joint Surgery-American Volume

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Increases in callus formation and mechanical strength of healing fractures in old rats treated with parathyroid hormone

Cited by 115 publications

References 14 publications

The effect of shock wave treatment at the tendon–bone interface—an histomorphological and biomechanical study in rabbits

The effect of shock wave treatment at the tendon–bone interface—an histomorphological and biomechanical study in rabbits

Intermittent parathyroid hormone (1?34) enhances mechanical strength and density of new bone after distraction osteogenesis in rats

2011 AOA Symposium: Tissue Engineering and Tissue Regeneration

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