Increasing Rhynchostylis retusa’s Callus Formation by Immersing Explant in Ascorbic Acid and Planting on Activated Charcoal Contained Medium

Climate changes due to global warming affect the productivity of forage crops such as maize. However, the productivity of maize could be maximized by improving the plant’s adaptation and tolerance through breeding, using tissue culture. This study aims to determine the effect of explant types and light conditions on callus induction of srikandi putih maize. It was conducted at the Laboratory of Forage and Pasture Science, Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta. Furthermore, it was carried out using a completely randomized design in a factorial pattern. There were two treatment factors namely explant types, which consisted of roots, stems, and leaves, and light conditions consisting of darkness and light. The results showed that callus induction occurred in the stem and root of explants at 60%, but not in the leaves. It also showed that the stem explants produced a higher fresh weight of callus at 239.6 mg compared to the roots which produced 77.6 mg. The dark condition also produced a 49.3% higher amount of callus with fresh weight of 115.1 mg. Meanwhile, the light condition produced 30.7% higher amounts of callus with fresh weight of 96.3 mg. It was concluded that all treatments showed a compact texture of callus, but the use of stem explants in dark conditions was the best treatment.

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“…The appearance began with swellings, and indicated the presence of cell activity [14]. These swellings later formed into clumps [15].…”

Section: Resultsmentioning

confidence: 99%

The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.)

Suhartanto

Astutik

Umami

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Apart from leaves, roots also have the potential to be used as explants. This is because cells in orchid roots have an excellent response to differentiation processes, especially adventitious roots, such as root culture in Cyrtopodium paludicolum orchids (Picolotto et al 2017), Rhynchostylis retusa's orchids (Kurniasari et al 2019). Consequently, the purpose of this study to characterize C. rochussenii orchids from Bukit Rimbang and Bukit Baling Wildlife Sanctuary, Riau, as a source of tissue culture explants in support of ex-situ conservation efforts.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Potential of Coelogyne rochussenii Orchids from Bukit Rimbang and Bukit Baling Wildlife Sanctuary as Explant Source

Heriansyah

Marlina

2021

JSL

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Conservation is an effort to return natural resources to their habitat to restore the ecosystem balance, which can be done in-situ and ex-situ. Coelogyne rochussenii orchid conservation efforts are essential to maintain its sustainability. The purpose of this study was to characterize C. rochussenii orchids from Bukit Rimbang and Bukit Baling Wildlife Sanctuary as a source of tissue culture explants to support ex-situ conservation efforts. Orchid plant samples were obtained through exploration in three locations with an altitude of 92 masl, and then the characterization of leaf morphology, pseudobulbs, roots, and fruit were carried out. The characterization results showed that the young pseudobulbs, young leaves, healthy roots, and physiologically ripe fruits of the C. rochussenii orchids obtained could be used as a source of explants to support ex-situ conservation efforts.Keywords: ex-situ conservation, physiologically mature, young pseudobulbs

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In Vitro Conservation and Propagation of Endangered Ethno-Medicinal Orchids from the Northeast Region of India

Mutum,

Chanu,

Khanganba

et al. 2023

Sustainable Development and Biodiversity

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Increasing Rhynchostylis retusa’s Callus Formation by Immersing Explant in Ascorbic Acid and Planting on Activated Charcoal Contained Medium

Cited by 3 publications

References 10 publications

The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.)

The effect of explants and light conditions on callus induction of srikandi putih maize (Zea mays L.)

Characterization and Potential of Coelogyne rochussenii Orchids from Bukit Rimbang and Bukit Baling Wildlife Sanctuary as Explant Source

In Vitro Conservation and Propagation of Endangered Ethno-Medicinal Orchids from the Northeast Region of India

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