Indium Tin Oxide-Polyaniline Biosensor: Fabrication and Characterization

Abstract

Abstract:In this study, a novel indium tin oxide (ITO)-polyaniline (Pani) biosensor was designed, fabricated, and characterized. Initial testing was conducted for the detection of bovine viral diarrhea virus (BVDV). The biosensor design was based upon the specific nature of antibodies to capture the target virus, and the conductive properties of self-doped Pani to translate the antibody-antigen binding into a quantifying signal. The first part of the study was to assess the feasibility of the self-doped Pani to be incorporated into the biosensor design by evaluating its several parameters, such as conductivity, physical structure, thermogravimetric properties, and antibody-binding properties. The second part of the paper highlights the fabrication of the ITO-Pani biosensor to detect the presence of bovine viral diarrhea virus (BVDV) in pure culture. Although only BVDV culture was tested in this study, the biosensor is versatile for the detection of other pathogen of interest by changing the specificity of the antibodies.

Liquid phase exfoliation of MoS2 and WS2 in aqueous ammonia and their application in highly efficient organic solar cells

“…allows increasing the hydrophilicity of surfaces. 19 The physical properties of the exfoliated nanosheets were characterised via a range of techniques including Raman, UV-vis, photoluminescence (PL), X-ray photoelectron spectroscopy (XPS), and ultraviolet photoelectron spectroscopy (UPS). The morphological and structural properties of the nanosheets were also investigated using atomic force microscopy (AFM) and high-resolution transmission electron microscopy (HR-TEM).…”

Amperometric glucose biosensor by means of electrostatic layer-by-layer adsorption onto polyaniline-coated polyester films

“…All of these methods have their own merits and demerits. Hence, many studies have been carried out to increase their individual merits [13][14][15][16][17][18][19][20][21][22].…”

A Resistance Based Biosensor That Utilizes Conductive Microfibers for Microbial Pathogen Detection

“…Constant potential amperometry (0.5 V), where a constant potential is applied and the current is measured, was used and the current values were recorded at fixed time intervals over 15 minutes. Previous studies have demonstrated that a potential of 0.2 V to 0.8 V is a preferable input signal when dealing with whole cells or other biological elements [24][25][26][27]. Based on this information, the potential of 0.5 V was arbitrarily selected.…”