Indole acetic acid (IAA) hormone production by endophytic bacteria isolate from Cinchona plant (Cinchona ledgerina Moens.) root

Anugrah, Fauzi Akhbar; Fanany, Rizal; Putra, Satrio Anggoro; Masita, Rahmi; Safitri, Desi Yulia

doi:10.1063/5.0052923

Cited by 4 publications

(6 citation statements)

References 13 publications

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“…TLC report of Bacillus paranthracis and Staphylococcus xylosus showed pink colour spot compared to standard IAA at the Rf value with respect to the authentic IAA (0.92). Previous report confirmed the presence of IAA with Rf values ranges from 0.69 to 0.95 (2,3,9,10,18) . It was confirmed that Bacillus paranthracis and Staphylococcus xylosus producing potential IAA with retention factor 0.91 and 0.95 respectively whereas Bacillus thuringienesis and Bacillus cereus gave no significant results shown in Figure 3 and Table 3 .…”

Section: Thin Layer Chromatographymentioning

confidence: 68%

“…Optimization of YMD media enhanced with carbon source, nitrogen source, tryptophan concentration, pH, temperature and incubation time was used for improved yield of IAA. The concentration of IAA was assessed using Salkowski method at 30 and 120 minutes; (12)(13)(14)(15) the values were represented by statistical analysis…”

Section: Optimization Of Ymd Media For Iaa Productionmentioning

confidence: 99%

“…The crude ethyl acetate extract was used for TLC analysis and this crude sample was dissipated in rotatory evaporator at 40 • C under vacuum condition, diluted with 2 ml of methanol for HPLC analysis. Most of the studies stated that ethyl acetate was used to extract the sample for analysis (9,10,15) .…”

Section: Extraction and Purificationmentioning

confidence: 99%

“…To consider this, researchers focus on an application study towards the synthesis of biological active phytohormones for development and growth of plants (1) . Several studies proved that endophytic bacteria can synthesis potential IAA, a metabolite of the L-tryptophan which promotes agricultural crops (2,3) .…”

Section: Introductionmentioning

confidence: 99%

“…The most significant and dynamic phytohormone is indole-3-acetic acid, controls different natural interaction for plant development (10) . Agrobacterium fabrum, Agrobacterium tumefaciens, Acinetobacter radioresistant, Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Brevibacillus brevis, Burkholderia cepacia, Pseudomonas sp, Paenibacillus barengoltzii and Lysinibacillus fusiformis are reported as common endophytic bacteria in various plant species that produce IAA (3,9) .…”

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Indole Acetic Acid of Endophytic Bacteria isolated from Kalanchoe pinnata (Lam.) in Optimized Media

Renugadevi¹,

Das²,

Sowndharya³

et al. 2022

IJST

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Objectives: To optimize, quantify and characterize indole acetic acid (IAA) production from endophtic bacteria isolated from the leaves of Kalanchoe pinnata (Lam.). Methods: The selected endophytic bacteria are identified by biochemical, morphological and 16SrRNA sequencing analysis. Endophytes are screened for IAA using Salkowski reagent and media optimization is carried out for maximum IAA yield and quantification was done with UV Spectrophotometer at 30 and 120 minutes. The mean ± standard error of each sample for three triplicates is calculated from mean value of the samples followed by normal distribution. The characterization of IAA was studied with ethyl acetate extract of endophytes using TLC and HPLC. Findings: The selected endophytes are identified as Bacillus thuringiensis, Bacillus paranthracis, Staphylococcus xylosus and Bacillus cereus. Optimized yeast malt dextrose (YMD) broth containing mannitol, ammonium nitrate, and 0.2% of L-tryptophan at pH 8 and 30• C temperature

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Section: Thin Layer Chromatographymentioning

confidence: 68%

Section: Optimization Of Ymd Media For Iaa Productionmentioning

confidence: 99%

Section: Extraction and Purificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production and Characterization of Indole Acetic Acid of Endophytic Bacteria isolated from Kalanchoe pinnata (Lam.) in Optimized Media

Renugadevi¹,

Das²,

Sowndharya³

et al. 2022

IJST

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Artemisia annua growth stimulation by IAA bacterial endophyte producer from Cinchona ledgeriana root

Zubaidah

Masita

Anugrah

2023

AIP Conference Proceedings

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Identification and Optimization of Indole-3-Acetic Acid Production of Endophytic Bacteria and Their Effects on Plant Growth

Khianngam

Meetum

Chiangmai

et al. 2023

tlsr

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Indole-3-acetic acid (IAA) is one of the most physiologically active auxins produced by rhizobacteria and is potentially applied for agriculture. Two endophytic bacteria, VR2 and MG9, isolated from the root of Chrysopogon zizanioides (L.) collected at Cha-Am, and the leaf of Bruguiera cylindrica (L.) Blume collected from a mangrove forest at Ban Laem, Phetchaburi Province, Thailand, were taxonomic characterised based on their phenotypic characteristics and 16S rRNA gene analysis. Strain VR2 was closely related to Enterobacter hormaechei CIP 103441T (99.6% similarity), while strain MG9 was closely related to Bacillus aryabhattai B8W22T (99.9% similarity). Consequently, they were identified as Enterobacter hormaechei and Bacillus aryabhattai, respectively. The IAA production of VR2 and MG9 strains are determined and applied to rice seeds for their root and shoot germination. Strains VR2 and MG9 greatly produced a yield of IAA, 246.00 and 195.55 µg/mL in 1,000 µg/mL of L-tryptophan at pH 6 for 48 h. They showed no significant differences in IAA to root and shoot development. However, the bacterial IAA exhibited potential nearby synthetic IAA, which had a significant effect compared to the control. IAA produced from these two strains might preferably trim down the use of synthetic IAA and could contribute to sustainable agriculture.

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Indole acetic acid (IAA) hormone production by endophytic bacteria isolate from Cinchona plant (Cinchona ledgerina Moens.) root

Cited by 4 publications

References 13 publications

Production and Characterization of Indole Acetic Acid of Endophytic Bacteria isolated from Kalanchoe pinnata (Lam.) in Optimized Media

Production and Characterization of Indole Acetic Acid of Endophytic Bacteria isolated from Kalanchoe pinnata (Lam.) in Optimized Media

Artemisia annua growth stimulation by IAA bacterial endophyte producer from Cinchona ledgeriana root

Identification and Optimization of Indole-3-Acetic Acid Production of Endophytic Bacteria and Their Effects on Plant Growth

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