Induced spawning in bream, Abramis brama (L.), using carp and bream pituitary extract and hCG

Abstract

Induced spawning in bream, Abramis brama (L), was studied using acetone‐dried common carp pituitary (CP) and bream pituitary (BP) with or without the addition of human chorionic gonadotrophin (hCG). The total dose administered to fish was of 5.0 mg kg−1 BP or 4.0 mg kg−1 CP with or without 2000‐2200 IU hCG kg−1 for females and 2.5 mg kg−1 BP or 2.0 mg kg−1 CP with or without 1000–1100 IU HCG kg−1 for males. In all male treated groups 100% of spermiation was observed: in females the most effective method was a triple injection with hCG and carp pituitary, resulting in 79% of females ovulated (over 68% of eyed eggs). Biological quality of eggs, expressed as a percentage of eyed eggs, was negatively correlated with time elapsing between resolving (final) injection and ovulation. Spawning success, expressed as a value of Se (spawning effectiveness coefficient), was higher in fish treated with triple injection.

Induced spawning in bream (Abramis brama L.) using pellets containing GnRH

“…For this reason many hormonal treatments are used to stimulate gamete maturation in commercial cyprinids. One of the most commonly applied spawning agents is carp pituitary extract (CPE) (Yaron et al, 1984;Thalathiah et al, 1988), in same cases with addition of human chorionic gonadotropin (hCG) (Kucharczyk et al, 1997a). The good results of induced ovulation in cyprinid fish were also obtained after hormonal stimulation by a synthetic analogue of gonadotropin-releasing hormone (GnRH), frequently with strong dopamine antagonists (Yaron, 1995;Barth et al, 1997).…”

“…Injections of hCG were intramuscular in the dorsal region of the body (Thalathiah et al, 1988). Injections of pituitary (Kucharczyk et al, 1997a) and ovopel (Horvath et al, 1997) extracts were intraperitoneal at the base of the pelvic fin. Before manipulations fish were anaesthetised with 2-phenoxyethanol (0.5 ml per l).…”

“…Artificial spawning is necessary to develop techniques of genome engineering and banking in bream (Kucharczyk, 2002). The aim of the present study was to test ovopel as a spawning agent in artificial reproduction of wild matured common bream (Abramis brama L.), in comparison with CPE and hCG stimulation which worked for common bream very well (Kucharczyk et al, 1997a).…”

Induced spawning of the endangered Neotropical species Steindachneridion parahybae (Siluriformes: Pimelodidae)

“…A summary of the doses used in this protocol is shown in Table 1. The use of hCG combined with pituitary extracts has been used in fish species (Thalathiah et al, 1988;Kucharczyk et al, 1997;Zohar & Mylonas, 2001;Leonardo et al, 2004) and has proven to be a very successful and consistent strategy in catfish species (Thalathiah et al, 1988).…”

Characteristics of the reproduction effectiveness of four Hungarian breeding lines of carp Cyprinus carpio (L.)

“…Jamiński (2005) also observed a high percentage of females from line 7 ovulating after treatment with Ovopel (90 %). The author indicated that the spawning effectiveness coefficient (Se; Kucharczyk et al 1997) for line 7 was considerably lower compared to the French line F and the Lithuanian lines B and V. Cejko (2007) indicated that within line 7, the percentage of ovulating females was higher after hypophysation than after treatment with Ovopel, whereas the egg weight expressed in grams and as a percentage of female body weight was higher after stimulation with Ovopel (the quality of eggs obtained after treatment with each of the stimulators did not vary). Białowąs et al (2008) reported a very low percentage of ovulating female (35-45 %) in line 7 and a very low relative fecundity for this line, namely 80-90 (thousand eggs/ kg -1 female body weight).…”