Induced spawning in bream (Abramis brama L.) using pellets containing GnRH

Abstract

Wild spawners of common bream, Abramis brama, were caught in the Kortowskie Lake (north Poland) and transported to a hatchery for artificial spawning. Fish were hormonally induced using GnRH analogue combined with metoclopramide (ovopel). The results of bream reproduction in captivity were compared with fish treated with the combination of hCG and CPE and with control group injected saline. Males from treated groups produced significantly more milt (over 4.3 ml/kg vs. 2.1) of better qualities: spermatozoon concentration (over 9.3 × 10 9 vs. 6.8) and motility (over 85% vs. 62). Females from the control group did not spawn whereas those from hormonally induced groups ovulated: 62% after CPE treatment and 100% after GnRHa treatment. Generally, the fish after ovopel stimulation showed the best hatchery parameters.

Artificial spawning of common tench Tinca tinca (Linnaeus, 1758), obtained from wild and domestic stocks

“…Differences in the latency time of tench were observed in the case of different spawning agents, but not in the case of fish origin. CPH usually involves a shorter latency time than Ovopel, and this was noted not only in the case of cyprinids (Brzuska 2005;Kucharczyk et al 2005;Krejszeff et al 2008) but also for percids (Kucharczyk et al 1996Szczerbowski et al 2009). Domesticated fish were reported to be able to spawn under controlled conditions without hormonal stimulation, and the latency time was much shorter .…”

“…Artificial reproduction of cyprinids is possible mainly after hormonal stimulation (Kucharczyk et al 1997a(Kucharczyk et al , 2005Brzuska 2005;Szabo et al 2002;Linhart et al 2006;Krejszeff et al 2008Krejszeff et al , 2009Podhorec and Kouril 2009;Yaron et al 2009;). The application of different spawning agents and doses resulted in different ovulation rates and gametes' quality (Glogowski et al 1997(Glogowski et al , 1999Babiak et al 1998;Kucharczyk et al 2005Kucharczyk et al , 2007Cejko et al 2010), which was usually caused by different interactions on the fish organism.…”

“…The application of different spawning agents and doses resulted in different ovulation rates and gametes' quality (Glogowski et al 1997(Glogowski et al , 1999Babiak et al 1998;Kucharczyk et al 2005Kucharczyk et al , 2007Cejko et al 2010), which was usually caused by different interactions on the fish organism. The largest differences were noted when fish from different stocks were reproduced ) as well as different spawn forms of one species , while multi-batch spawners are much more difficult to reproduce (Kucharczyk et al 1997c).…”

Untitled

“…Fish reproduction during the reproduction period does not usually pose any difficulties in other cyprinids. There is only a short period of gradual temperature increase required as well as administering the right hormonal agents at the right doses (Kucharczyk et al, 2005;Nowosad et al, 2014a). In some cases, for example, in the reproduction of domesticated shoals of some cyprinids species, it is enough to control the thermal conditions to achieve effective reproduction under controlled conditions .…”

Effect of time after hormonal stimulation on semen quality indicators of common carp, <I>Cyprinus carpio</I> (Actinopterygii: Cypriniformes: Cyprinidae)

“…Among commercially available hormonal preparations, the synthetic analogue Ovopel [(D-Ala 6 , Pro 9 NEt)--mGnRH+metoclopramide] is recommended for reproduction of cyprinid- (Horváth et al 1997, Kucharczyk et al 2005 and non-cyprinid fish (Brzuska 2001, Kucharczyk et al 2001, Król et al 2009). Recently conducted studies also indicate that Ovopel has an application in the reproduction of rheophilic cyprinid fishes i.e., asp, Aspius aspius (L.); ide, Leuciscus idus (L.); or chub, Squalius cephalus (L.) (see Cejko et al 2008, Żars-ki et al 2009).…”