Induction of alkaline phosphatase activity by l-ascorbic acid in human osteoblastic cells: a potential role for CK2 and Ikaros

Son, Eunwha; Hang, Đo Thi Thu; Joo, Haemi; Pyo, Suhkneung

doi:10.1016/j.nut.2007.06.013

Cited by 19 publications

(8 citation statements)

References 38 publications

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“…The present results demonstrate that CK2 inhibitor, DRB, inhibited iNOS expression and restored the reduced DNA binding activity of Ikaros induced by the LPS/IFN-g treatment. Our previous data also demonstrated that siRNA-mediated depletion of the CK2a completely restored the Ikaros DNA binding activity [42]. In addition, the overexpression of the CK2a kinase-deficient mutant induced a marked inhibition of NO production and iNOS expression.…”

Section: Discussionmentioning

confidence: 61%

Ikaros negatively regulates inducible nitric oxide synthase expression in macrophages: Involvement of Ikaros phosphorylation by casein kinase 2

Cho

Huh

Song

et al. 2008

Cell. Mol. Life Sci.

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Ikaros is known as a critical regulator of lymphocyte development. We examined the regulatory role of Ikaros in LPS/IFN-gamma-induced inducible nitric oxide synthase (iNOS) expression by macrophages. Our results showed that IK6 (Ikaros dominant negative isoform) induction increases the iNOS expression. Ikaros DNA binding activity on the iNOS promoter was decreased, and a mutation of the Ikaros-binding site on the iNOS promoter resulted in an increase in LPS/IFN-gamma-induced iNOS expression. LPS/IFN-gamma increased the histone (H3) acetylation on the Ikaros DNA binding site. These results suggest that Ikaros acts as a negative regulator on iNOS expression. Treatment with a casein kinase 2 (CK2) inhibitor reversed LPS/IFN-gamma-induced decrease in Ikaros DNA binding activity. Moreover, overexpression of kinase-inactive CK2 decreased iNOS expression and a significant amount of CK2alpha1 translocated into the nucleus in LPS/IFN-gamma-treated cells. Overall, these data indicate that LPS/IFN-gamma decreases the Ikaros DNA binding activity via the CK2 pathway, resulting in an increase of iNOS expression.

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Section: Discussionmentioning

confidence: 61%

Ikaros negatively regulates inducible nitric oxide synthase expression in macrophages: Involvement of Ikaros phosphorylation by casein kinase 2

Cho

Huh

Song

et al. 2008

Cell. Mol. Life Sci.

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“…Another study on mesenchymal stem cells revealed that ascorbate supplementation (0-500 μM) had almost no effect on growth and proliferation until passage 3 (Choi et al, 2008). Son et al demonstrated that ascorbate supplementation (60 and 125 μM) did not affect the collagen expression (Son et al, 2007). To rule out an effect of the different culture conditions on matrix expression, we agree that it would have been better to culture all cells with the same supplementations.…”

Section: S Pauly Et Al Human Rotator Cuff Tendon Cell Culturesmentioning

confidence: 64%

Characterization of tendon cell cultures of the human rotator cuff

Pauly

Klatte²,

Strobel³

et al. 2010

eCM

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Rotator cuff tears are common soft tissue injuries of the musculoskeletal system that heal by formation of repair tissue and may lead to high retear rates and joint dysfunction. In particular, tissue from chronic, large tendon tears is of such degenerative nature that it may be prone to retear after surgical repair. Besides several biomechanical approaches, biologically based strategies such as application of growth factors may be promising for increasing cell activity and production of extracellular tendon matrix at the tendon-to-bone unit. As a precondition for subsequent experimental growth factor application, the aim of the present study was to establish and characterize a human rotator cuff tendon cell culture.Long head biceps (LHB)-and supraspinatus muscle (SSP)-tendon samples from donor patients undergoing shoulder surgery were cultivated and examined at the RNA level for expression of collagen type-I, -II and -III, biglycan, decorin, tenascin-C, aggrecan, osteocalcin, tenomodulin and scleraxis (by Real-time PCR). Finally, results were compared to chondrocytes and osteoblasts as control cells.An expression pattern was found which may reflect a human rotator cuff tenocyte-like cell culture. Both SSP and LHB tenocyte-like cells differed from chondrocyte cell cultures in terms of reduced expression of collagen type-II (p≤0.05) and decorin while higher levels of collagen type-I were seen (p≤0.05). With respect to osteoblasts, tenocytelike cells expressed lower levels of osteocalcin (p≤0.05) as well as tenascin C, biglycan and collagen type-III. Expression of scleraxis, tenomodulin and aggrecan was similar between all cell types.This study represents a characterization of tenocytelike cells from the human rotator cuff as close as possible. It helps analyzing their biological properties and allows further studies to improve production of tendon matrix and osteofibroblastic integration at the tendon-bone unit following tendon repair.

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“…The expression of COL I, ALP, BMP-2, OPG, and M-CSF genes, which are typical osteoblastic phenotypes, [37][38][39] were clearly observed after 6 days of cells cultured on both materials.…”

mentioning

confidence: 94%

Innovative macroporous granules of nanostructured‐hydroxyapatite agglomerates: Bioactivity and osteoblast‐like cell behaviour

Laranjeira

Fernandes

Monteiro

2010

J Biomedical Materials Res

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To modulate the biological response of implantable granules, two types of bioactive porous granules composed of nanostructured-hydroxyapatite (HA) agglomerates and microstructured-HA, respectively, were prepared using a polyurethane sponge impregnation and burnout method. The resulting granules presented a highly porous structure with interconnected porosity. Both types of granules were characterized using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and mercury intrusion porosimetry. Results showed that nanostructed-HA granules presented higher surface area and porosity than microstructured-HA granules. In vitro testing using MG63 human osteoblast-like cells showed that on both types of surfaces cells were able to adhere, proliferate, and migrate through the macropores, and a higher growth rate was achieved on nanostructured-HA granules than on microstructured-HA granules (76 and 40%, respectively). In addition, these cells maintained similar expression levels of osteoblastic-associated markers namely collagen type I, alkaline phosphatase, bone morphogenetic protein-2, macrophage colony-stimulating factor, and osteoprotegerin. These innovative nanostructured-HA granules may be considered as promising bioceramic alternative matrixes for bone regeneration and drug release application.

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Induction of alkaline phosphatase activity by l-ascorbic acid in human osteoblastic cells: a potential role for CK2 and Ikaros

Cited by 19 publications

References 38 publications

Ikaros negatively regulates inducible nitric oxide synthase expression in macrophages: Involvement of Ikaros phosphorylation by casein kinase 2

Ikaros negatively regulates inducible nitric oxide synthase expression in macrophages: Involvement of Ikaros phosphorylation by casein kinase 2

Characterization of tendon cell cultures of the human rotator cuff

Innovative macroporous granules of nanostructured‐hydroxyapatite agglomerates: Bioactivity and osteoblast‐like cell behaviour

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