Induction of apoptosis by Uncaria tomentosa through reactive oxygen species production, cytochrome c release, and caspases activation in human leukemia cells

Cheng, An-Chin; Jian, Cheng; Huang, Yu‐Ting; Lai, Ching-Shu; Hsu, Ping-Chi; Pan, Min‐Hsiung

doi:10.1016/j.fct.2007.05.016

Cited by 56 publications

(40 citation statements)

References 56 publications

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“…Moreover, it has been stated that both healthy and malignant cells achieve their termination via apoptosis or necrosis, and that the balance of ROS is quite crucial in determining which of these two pathways will be unfolded (Skulachev, 2000;Pelicano et al, 2004;El-Najjar et al, 2009). In the present work we observed an important correlation between the antitumoral properties of UT and its potential as a free radical scavenger and as a provider of reactive oxygen species (ROS) homeostasis, corroborating previous reports (Wirth and Wagner, 1997;Choi et al, 2002;Pilarski et al, 2006;Cheng et al, 2007). More than an antioxidant effect per se as demonstrated by DPPH assay, our results show that UT extract is able to reduce hepatic SOD activity in the highest dose (100 mg kg −1 ).…”

Section: Parametersupporting

confidence: 94%

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Dreifuss

Bastos-Pereira

Ávila

et al. 2010

Journal of Ethnopharmacology

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Section: Parametersupporting

confidence: 94%

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Dreifuss

Bastos-Pereira

Ávila

et al. 2010

Journal of Ethnopharmacology

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“…The aqueous extracts of this plant showed no cytotoxic activity in the ovary cells of Chinese hamsters (Santa Maria et al, 1997). However, in another work, the ethyl acetate fraction obtained from U. tomentosa induced apoptosis in human leukemic HL-60 cells (Cheng et al, 2007). The ethanol extracts obtained from leaves of Pavetta crassipes exhibited antiprotozoal, antimicrobial and antitumor activities and an alkaloid fraction obtained from this plant showed antiproliferative activity against four strains of carcinomas (U373, PC3, MXT and A549) and normal cell lines (NHDF and MRC-5) (Baldéa et al, 2010).…”

Section: Discussionmentioning

confidence: 97%

Assessment of the in vitro and in vivo genotoxicity of extracts and indole monoterpene alkaloid from the roots of Galianthe thalictroides (Rubiaceae)

Fernandes

Garcez

Mantovani

et al. 2013

Food and Chemical Toxicology

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Roots of Galianthe thalictroides K. Schum. (Rubiaceae) are used in folk medicine in the State of Mato Grosso do Sul, Brazil, for treating and preventing cancer. To gain information about the genotoxicity of extracts (aqueous and EtOH), the CHCl₃ phase resulting from partition of the EtOH extract and the indole monoterpene alkaloid 1 obtained from this plant. The genotoxicity of 1 and extracts was evaluated in vivo through the Drosophila melanogaster wing Somatic Mutation and Recombination Test - SMART, while in vitro cytotoxic (MTT) and Comet assays were performed only with alkaloid 1. The results obtained with the SMART test indicated that the aqueous extract had no genotoxic activity. The EtOH extract was not genotoxic to ST descendants but genotoxic to HB ones. The CHCl₃ phase was genotoxic and cytotoxic. Alkaloid 1 showed significant mutational events with SMART, in the cytotoxicity assay (MTT), it showed a high cytotoxicity for human hepatoma cells (HepG2), whereas for the Comet assay, not showing genotoxic activity. The ethanol extract was shown to be genotoxic to HB descendants in the SMART assay, while the results obtained in this test for the monoterpene indole alkaloid 1 isolated from this extract.

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“…Cytosolic cytochrome c initiates caspase-dependent apoptosis by first binding to Apaf-1. In the presence of dATP, this cytochrome c/Apaf-1 complex activates caspase-9 and caspase-3, thereby triggering apoptosis [34][35] . To determine whether PSⅠ-induced apoptosis followed caspase activation, we examined the effects of PSⅠon the expression levels of procaspase-9 and procaspase-3 by Western blot analysis.…”

Section: Discussionmentioning

confidence: 99%

Paris saponin I induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells

Xiao

Dai

et al. 2011

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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The aim of this study was to investigate the effect of Paris saponin I (PS I) on human gastric carcinoma cell growth and apoptosis and to explore the potential mechanisms. The proliferation of SGC7901 cells was monitored by the MTT cell viability assay, while the nuclear morphology of apoptotic cells was assessed by Hoechst 33258 staining. Flow cytometry was performed to analyze the cell cycle progression of propidium iodide (PI)-stained SGC7901 cells and the apoptotic rate of annexin V/PI-stained cells. Western blotting was used to examine the expression of several cell cycle proteins, including cyclin B1 and Cdk1, and the apoptosis-regulated proteins Bcl-2, Bax, cytochrome c, procaspase-9, and procaspase-3. The MTT assay demonstrated that PS I could induce significant dose- and time-dependent inhibition of SGC7901 cell proliferation. Marked morphological changes, including condensation of chromatin, nuclear fragmentation and apoptotic bodies were clearly shown on Hoechst 33258 staining. PSI treatment also resulted in the disruption of the cell cycle at G₂/M and the induction of apoptosis. Following PSI treatment, the cell cycle-related proteins cyclin B1 and Cdk1 were down-regulated. Expression of the pro-apoptotic protein Bax was increased, while anti-apoptotic protein Bcl-2 decreased. PSI treatment resulted in elevated cytoplasmic cytochrome c and activation of the apoptotic proteases caspase-9 and caspase-3. These data indicate that PS acts as an inhibitor of proli I feration in SGC7901 cells by inducing cell cycle arrest and mitochondria-dependent apoptosis. PSI is a potential therapeutic agent against human gastric carcinoma.

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Induction of apoptosis by Uncaria tomentosa through reactive oxygen species production, cytochrome c release, and caspases activation in human leukemia cells

Cited by 56 publications

References 56 publications

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Antitumoral and antioxidant effects of a hydroalcoholic extract of cat's claw (Uncaria tomentosa) (Willd. Ex Roem. & Schult) in an in vivo carcinosarcoma model

Assessment of the in vitro and in vivo genotoxicity of extracts and indole monoterpene alkaloid from the roots of Galianthe thalictroides (Rubiaceae)

Paris saponin I induces G2/M cell cycle arrest and apoptosis in human gastric carcinoma SGC7901 cells

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