Induction of Betacyanin Synthesis and Pigment Accumulation in Cell Suspension Cultures of Portulaca.

Noda, Naonobu; Adachi, Tomohiko

doi:10.5511/plantbiotechnology.17.27

Cited by 11 publications

(8 citation statements)

References 32 publications

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“…Higher concentrations of 6-BA (10 mg·L −1 ) repressed betacyanin content and callus growth of S. salsa. The results indicate that the increase in betacyanin content with a combination of 6-BA and IAA is related to vigorous growth, which is consistent with such reports in seedlings (Piattelli and Imperato 1971) and calluses of Amaranthus tricolor (Bianco-Colomas and Hugues 1990), in cell suspension cultures of P. Americana (Hirano and Komamine 1994), and in Portulaca (Noda and Adachi 2000). Bhuiyan and Adachi (2003) also showed that cell growth and the accumulation of betacyanin were positively correlated.…”

Section: Discussionsupporting

confidence: 90%

“…For example, cytokinins increase betacyanin accumulation in Chenopodium album (Rudat and Göring 1995), Saussurea medusa , and Alternanthera brasiliana (Silva et al 2005), but addition of cytokinin decreases betacyanin accumulation in cell suspension cultures of Portulaca (Noda and Adachi 2000) and Mammillaria candida (Santos-Diaz et al 2005). The exact mechanism of regulation by growth regulators in betacyanin metabolism remains unclear.…”

Section: Introductionmentioning

confidence: 97%

“…The exact mechanism of regulation by growth regulators in betacyanin metabolism remains unclear. Previous studies have shown that cell cultures can be a useful tool for analyzing the regulatory mechanism of betalain biosynthesis and for the characterization of the enzymes involved (Noda and Adachi 2000).…”

Section: Introductionmentioning

confidence: 99%

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Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli

Zhao

Sun

Gao

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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Suaeda salsa calluses cultured in darkness for 28 d were transferred to Murashige and Skoog (MS) media containing various growth regulators under white light conditions for 10 d to investigate cell growth, betacyanin accumulation, and expression of dopa-4,5-dioxygenase (DODA). Callus growth was markedly promoted when 0.2 mg·L −1 2,4-D and 0.5 mg·L −1 6-BA were added to the MS medium. Surprisingly, of the auxins tested, IAA had no effect on betacyanin content, but 2,4-D strongly decreased betacyanin content. Betacyanin content was positively correlated with 6-BA concentrations in the range of 0.1-2.0 mg·L −1 . DODA mRNA levels were consistent with the response of betacyanin content to exogenous growth regulators. These results suggest that betacyanin metabolism in S. salsa calluses is regulated under white light conditions by growth regulators through the regulation of genes such as DODA that are involved in betacyanin synthesis.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 97%

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Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli

Zhao

Sun

Gao

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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“…Surprisingly, our primary results showed that light enhanced the betacyanin accumulation in S. salsa calli. Previous studies have shown that cell cultures can be a useful tool for analyzing the regulatory mechanism of betalain biosynthesis and for the characterization of the enzymes involved (Noda and Adachi 2000). In the present study, we introduced the C 3 halophyte S. salsa calli as a model system for studying the effect of light on betacyanin content and whether there is a correlation between DODA expression or activity and expression of tyrosinase and betacyanin content in the calli of S. salsa under light.…”

Section: Introductionmentioning

confidence: 98%

Light-regulated betacyanin accumulation in euhalophyte Suaeda salsa calli

Zhao

Sun

Chen

et al. 2010

Plant Cell Tiss Organ Cult

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Suaeda salsa calli cultured in darkness for 28 days were transferred to Murashige and Skoog (MS) media containing 0.5 mg l -1 Thidiazuron (TDZ) and 1.0 mg l -1 naphthaleneacetic acid (NAA) under different light conditions for 10 days to investigate effect of light on betacyanin accumulation and its relation to activity and expression of tyrosinase and expression of dopa-4,5-dioxygenase gene (DODA). Both light quality and quantity affected betacyanin synthesis of S. salsa calli. 80 lmol m -2 s -1 of white light intensity was optimal for cell growth and betacyanin accumulation of S. salsa calli. DODA mRNA levels and tyrosinase activity were consistent with the response of betacyanin content to different light quality. However, the protein levels of tyrosinase extracted from S. salsa calli response to different light quality were constant. These results suggest that betacyanin metabolism in S. salsa calli is regulated by different light quality through the regulation of genes such as DODA and tyrosinase activity but not via alteration of the protein amount of tyrosinase.

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“…Glucose concentration was measured using a glucose analyzer (model 2700, YSI Inc., Yellow Springs, OH, USA). Alanine concentration was analyzed by high-performance liquid chromatography (HPLC) as described previously (Meyer et al 1984;Noda and Adachi 2000) with modifications. Alanine was derivatized with phenylisothiocyanate to produce phenylthiocarbamyl alanine and then analyzed by HPLC under the following conditions: column, Wakosil-PTC, 4.0 mm · 250 mm (D) (Wako Pure Chemicals Inc., Japan), solvent A, 60 mM sodium acetate buffer (pH 6.0) in acetonitrile (94:6); and solvent B, 60 mM sodium acetate buffer (pH 6.0) in acetonitrile (40:60).…”

Section: Analysis Of Fermentation Productsmentioning

confidence: 99%

Reduction of lactate production in Lactococcus lactis, a combined strategy: metabolic engineering by introducing foreign alanine dehydrogenase gene and hemin addition

Wardani

Nagahisa

Shimizu

et al. 2006

World J Microbiol Biotechnol

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An approach to decreasing the lactate production in Lactococcus lactis by metabolic engineering is presented. The inhibitory effects of a low pH due to the accumulation of lactate on cell growth and nisin production in L. lactis are well known. To avoid such inhibitory effects, a new strategy by rerouting carbon flow was considered. In an effort to suppress lactate production, a new gene was introduced into L. lactis to create a novel pathway for alanine synthesis to reroute the metabolic flow of lactate. Alanine dehydrogenase (E.C.1.4.1.1) encoded by alaD from Bacillus sphaericus was expressed in L. lactis. The enzyme was expressed to a specific activity of nearly 0.39 U/mg protein in the transformant. Hemin addition was also considered to decrease the lactate production in L. lactis. The effect of hemin on the alanine production in the transformant was investigated. This study showed that using the combined strategy, stronger effects on lactate and alanine productions were observed in the transformant.

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Induction of Betacyanin Synthesis and Pigment Accumulation in Cell Suspension Cultures of Portulaca.

Cited by 11 publications

References 32 publications

Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli

Growth regulator-induced betacyanin accumulation and dopa-4,5-dioxygenase (DODA) gene expression in euhalophyte Suaeda salsa calli

Light-regulated betacyanin accumulation in euhalophyte Suaeda salsa calli

Reduction of lactate production in Lactococcus lactis, a combined strategy: metabolic engineering by introducing foreign alanine dehydrogenase gene and hemin addition

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