Induction of defense-related proteins by mixtures of plant growth promoting endophytic bacteria against Banana bunchy top virus

Harish, S.; Kavino, M.; Kumar, Naresh; Balasubramanian, P.; Samiyappan, R.

doi:10.1016/j.biocontrol.2009.06.002

Cited by 120 publications

(48 citation statements)

References 64 publications

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“…These results are in agreement with those of Kavitha and Jonathan [37] who found that the inoculation of tomato plants with FP strains caused significant increase in PO, PPO and PAL. Recent investigations of the mechanisms of biological control by plant-growth promoting fluorescent pseudomonads revealed that PGPR strains protect plants from pathogen attack through systemic protection by strengthening the epidermal and cortical cell walls with deposition of newly formed barriers beyond infection sites including callose, lignin and phenolics [38] and by activating defense genes encoding chitinase, PO, PPO and PLA and enzymes which are involved in the synthesis of phytoalexins [12]. PLA plays an important role in the biosynthesis of phenolics that are effective chemical barriers against pathogen infection.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, research has focused on other bacteria for control of plant parasitic nematodes. Because of their catabolic versatility, their excellent root colonizing ability and their capacity to produce a wide range of antiphytopathogenic metabolites, pseudomonads have received particular attention [1,9,10,11,12]. However, the available information on nematicidal activity of Egyptian bacterial isolates, particularly rhizofluorescent pseudomonads, is scant.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita

Gamal¹

2010

J Biofertil Biopestici

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A total of 52 Egyptian rhizosphere fluorescent pseudomonad isolates (ERFP) were screened for their nematicidal activity in vitro. The screening results showed an inhibitory effect on hatching of Meloidogyne incognita eggs ranging from 57 % to 100 %. Similar to the chemical nematicide Videt in vitro experiment, cultures or cell-free supernatants (CFS) of the ERFP isolates showed complete inhibition of egg hatching and killed 100 % of the J 2 . In glasshouse pot experiments, cultures of these isolates, CFS and the chemical nematicide showed reduction in root galling ranging from 81. 0-95.4, 61.3-84.5, 97.3%, respectively. Also, the reduction in nematode multiplication in soil was 91. 9-95.7, 83.5-84.5 and 96.5% for cultures, CFS and the chemical nematicide, respectively, compared to the positive control (nematode only). Furthermore, these isolates showed significant increases in plant growth characters, phenolic content and activities of the plant defense-related enzymes. Based on 16S rDNA sequences and API NE kits, two major clusters were observed; strains Ps 36 and Ps 54 appeared to fall within the variability range of P. putida, while Ps 21, Ps 22 and Ps 14 are likely to be strains of P. aeruginosa. The distinctness of Ps 36 and Ps 54 from Ps 14, Ps 21 and Ps 22 was supported by their physiological characteristics, such as the ability of Ps 14 and Ps 21 and Ps 22 to utilize mannitol, N-acetyl-glucose amine and adipate. Johnsen et al. [14]. DNA 16S region amplification was performed using the primer set 16SF-16SR [15]. The 16S rDNA was sequenced as described by Sanger et al. [16]. The 16S rDNA sequences were initially analyzed by using the program Blast (National Center Biotechnology Information, http//:www.ncbi.nml.nih.gov). Sequencing data obtained from different primers were collected together using the CAP program (Contig Assembly and Genomic Expression programs). The consensus sequence from the isolates and sequences of strains belonging to the same phylogenetic group and of other representatives of Pseudomonas strains (retrieved from the NCBI database) were aligned using the computer-program ClustalX [17]. The resulting trees were displayed with Tree View [18]. The phylogenetic tree was calculated using the neighbor-joining method [19] and Acinetobacter calcoaceticus and E. coli K12 were used as outgroups. Preparation of bacterial inocula and their cell -free culture supernatants (CFS)The pseudomonad isolates were grown separately for 36 h in King's B medium broth at 28°C on a rotary shaker at 150 rpm. Part of the cultures was kept without centrifugation for use at 4°C. The bacterial cultures mixture was prepared by mixing equal volumes of cell suspension of individual isolates. For preparation of cell-free culture filtrate, the other part of the bacterial cultures were centrifuged at 5,000 x g for 30 min; pellets were discarded and the supernatants were filtered through bacterial filter (0.22μm pore size) and kept for use at 4°C. Preparation of nematode inoculaA population of the root-knot nemat...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita

Gamal¹

2010

J Biofertil Biopestici

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“…β-1,3-glucanases), which contribute in releasing oligosaccharides that results in stimulating other defence reactions [14]. The ISR is induced to restrict of pathogens to outer plant root cortex [49] by increasing host cell wall density to restrict potential pathogen penetration [14], which is activated by expression of pathogenesis-related (PR) proteins, (chitinase and b-1,3-glucanase) and defence-related proteins, (peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase) and phenolic compounds [50]. Endophytes also induce ISR to plants to fight against viral pathogens.…”

Section: Biocontrol Mechanismmentioning

confidence: 99%

“…The reports show induction of ISR in tomato plants by endophytes, Bacillus subtilis IN937b, B. pumilus SE34, and B. amyloliquefaciens IN937a against cucumber mosaic cucumovirus (CMV) [51]. Several endophytes cause ISR in plants against banana bunchy top virus [50]. Also, the common endophyte genus Paenibacillus is known to produce soluble and volatile metabolites that may inhibit the pathogen growth [39].…”

Section: Biocontrol Mechanismmentioning

confidence: 99%

Exploring the Potential of Endophytes in Agriculture: A Minireview

Yadav¹

2017

APAR

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“…The primers used in this study were chosen among those that, previously, were successfully applied to differentiate genetic populations of various soil-borne microbes [21][22][23][24][25].…”

Section: Mini and Microsatellite Amplificationmentioning

confidence: 99%

Metagenomic profiles of soil microbiota under two different cropping systems detected by STRs-based PCR

Zaccardelli¹,

Villecco²,

Campanile³

et al. 2012

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In this research, soil microbial structures under a wheat triennial monoculture and horse beanwheat-horse bean succession were evidenced using a metagenomic approach. Polymorphism analysis of DNA extracted from soil samples collected at the end of the third year of the two crop successions, was performed by PCR, carried-out with six different primers designed on simple tandem repeats sequences. Readable profiles were obtained with M13 primer, from which no polymorphisms were detected, and with the primer (GACA) 4 , that gave distinctive patterns. Experimental findings suggest that metagenomic analysis performed by (GACA) 4 primer may be an easy and suitable method to discriminate microbial diversity of different crop successions. (GACA) 4 PCR-pattern indicate that soil microbiota changes are well correlated with crop succession.

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Induction of defense-related proteins by mixtures of plant growth promoting endophytic bacteria against Banana bunchy top virus

Cited by 120 publications

References 64 publications

Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita

Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita

Exploring the Potential of Endophytes in Agriculture: A Minireview

Metagenomic profiles of soil microbiota under two different cropping systems detected by STRs-based PCR

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