Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells

Wang, Hanning; Xiang, Jinzhu; Zhang, Wei; Li, Junhong; Wei, Qingqing; Liu, Zhong; Ouyang, Hongsheng; Han, Jianyong

doi:10.1038/srep27256

Cited by 44 publications

(44 citation statements)

References 52 publications

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“…Then, the pPGCs at E15, stage‐equivalent to the E8.5 in the mouse, commence migration towards the genital ridges and, at E18, they start to colonize the genital ridges . Recently, the induction of porcine PGCLCs (pPGCLCs) from porcine iPSCs was reported (Figure ) . Porcine iPSCs first are converted into EpiLCs in the presence of bFGF and activin A for 2 days.…”

Section: Induction Of Primordial Germ Cell‐like Cells In Non‐murine Mmentioning

confidence: 99%

“…56 Recently, the induction of porcine PGCLCs (pPGCLCs) from porcine iPSCs was reported ( Figure 4). 57 Porcine iPSCs first are converted into EpiLCs in the presence of bFGF and activin A for 2 days. Then, the disaggregated cultures are kept in the presence of four cytokines (BMP4, LIF, SCF, and EGF) to induce pPGCLCs.…”

Section: Induction Of Primordial Germ Cell-like Cells In Non-murinementioning

confidence: 99%

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Generation of germ cells from pluripotent stem cells in mammals

Hayashi

Kawaguchi

Durcova-Hills

et al. 2017

Reprod Medicine & Biology

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BackgroundThe germ cell lineage transmits genetic and epigenetic information to the next generation. Primordial germ cells (PGCs), the early embryonic precursors of sperm or eggs, have been studied extensively. Recently, in vitro models of PGC induction have been established in the mouse. Many attempts are reported to enhance our understanding of PGC development in other mammals, including human.MethodsHere, original and review articles that have been published on PubMed are reviewed in order to give an overview of the literature that is focused on PGC development, including the specification of in vivo and in vitro in mice, human, porcine, and bovine.ResultsMammalian PGC development, in vivo and in vitro, have been studied primarily by using the mouse model as a template to study PGC specification in other mammals, including human, porcine, and bovine.ConclusionThe growing body of published works reveals similarities, as well as differences, in PGC establishment in and between mouse and human.

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Section: Induction Of Primordial Germ Cell‐like Cells In Non‐murine Mmentioning

confidence: 99%

Section: Induction Of Primordial Germ Cell-like Cells In Non-murinementioning

confidence: 99%

Generation of germ cells from pluripotent stem cells in mammals

Hayashi

Kawaguchi

Durcova-Hills

et al. 2017

Reprod Medicine & Biology

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“…MSCs do not form teratomas when injected into donor testes and have even been shown to help reestablish the testicular niche following chemical damage (Cakici et al, 2013;Maghen et al, 2017;Vahdati et al, 2017). Male PGCLCs have also been produced from induced pluripotent stem cells (iPSC), which can also be derived from adult animals (Hayashi et al, 2011;Sasaki et al, 2015;Wang et al, 2016a). Further experiments are required to definitively demonstrate that these PGCLCs from different cell types can fulfill the functional definition of a germ cell, i.e.…”

Section: Intensity Of Selectionmentioning

confidence: 99%

Genome editing approaches to augment livestock breeding programs

Bishop

Eenennaam

2020

Journal of Experimental Biology

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The prospect of genome editing offers a number of promising opportunities for livestock breeders. Firstly, these tools can be used in functional genomics to elucidate gene function, and identify causal variants underlying monogenic traits. Secondly, they can be used to precisely introduce useful genetic variation into structured livestock breeding programs. Such variation may include repair of genetic defects, the inactivation of undesired genes, and the moving of useful alleles and haplotypes between breeds in the absence of linkage drag. Editing could also be used to accelerate the rate of genetic progress by enabling the replacement of the germ cell lineage of commercial breeding animals with cells derived from genetically elite lines. In the future, editing may also provide a useful complement to evolving approaches to decrease the length of the generation interval through in vitro generation of gametes. For editing to be adopted, it will need to seamlessly integrate with livestock breeding schemes. This will likely involve introducing edits into multiple elite animals to avoid genetic bottlenecks. It will also require editing of different breeds and lines to maintain genetic diversity, and enable structured crossbreeding. This requirement is at odds with the process-based trigger and event-based regulatory approach that has been proposed for the products of genome editing by several countries. In the absence of regulatory harmony, researchers in some countries will have the ability to use genome editing in food animals, while others will not, resulting in disparate access to these tools, and ultimately the potential for global trade disruptions.

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“…The differentiation approach used to generate PGCLCs from human EpiLCs served as template for the evaluation of a similar culture system in pigs using iPSCs. The PGCLC identity was supported by the expression of PGC (BLIMP1, PRDM14, STELLA) and pluripotency markers (OCT4, SOX2), an epigenetic status similar to in vivo PGCs, and transcriptome and gene ontology analyses that revealed a gamete production scheme (Wang et al, ). Also, the recently developed pig EPSCs produced PGCLCs in vitro (Gao et al, ).…”

Section: In Vitro Differentiation Of Gametes From Pluripotent Stem Cellsmentioning

confidence: 99%

Gametes from stem cells: Status and applications in animal reproduction

Goszczynski

Denicol

Ross

2019

Reprod Domestic Animals

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In vitro gamete differentiation could revolutionize animal production by decreasing generation intervals, increasing the number of gametes per animal and facilitating the dissemination of elite genetics. In addition, it could help to develop new strategies for the conservation of endangered species. The recent in vitro reconstitution of germ cell development in mice has inspired researchers to invest their best efforts into reproducing this achievement in livestock species. With this goal in mind, multiple differentiation approaches and cell sources have been evaluated. The degree of success in these evaluations varies according to the species and the stage of development studied, but, in general, partially positive results have been obtained. Evidence suggests that although functional gametes with true reproductive potential are still to be obtained, it is a matter of time before this goal is achieved. K E Y W O R D S embryo, gametogenesis, stem cells | 23 GOSZCZYNSKI et al.

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Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells

Cited by 44 publications

References 52 publications

Generation of germ cells from pluripotent stem cells in mammals

Generation of germ cells from pluripotent stem cells in mammals

Genome editing approaches to augment livestock breeding programs

Gametes from stem cells: Status and applications in animal reproduction

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