“…6) indicating a negative surface charge on synthesized NPs. Similar results were obtained by ZnO NRs synthesized using Santalum album leaf extract with zeta potential value of − 13 mV [68]. It should be noted that the particles with zeta potential values more positive than + 30 mV or more negative than − 30 mV are considered to be stable [69].…”
Section: Surface Charge and Stability By Zeta Potentialsupporting
Aedes aegypti mosquito species is a primary vector for dengue, chikungunya and Zika infections, and vector control is the only key approach for reducing their transmission. The present study emphasizes on environmental friendly approach for the fabrication of zinc oxide nanorods (ZnO NRs) using aqueous extract of Lumnitzera racemosa flower buds (LB) as a reducing and stabilizing agent. ZnO NRs were examined by UV-Vis spectroscopy with characteristic absorbance band at 373.82 nm and bandgap of 3.25 eV. FT-IR analysis revealed the functional groups associated with ZnO NRs. The crystallinity of LB-ZnO NRs was further investigated using X-ray diffraction. The Zeta potential displayed a surface charge of − 23.5 mV on NRs. Energy dispersive spectra analysis and SEM analysis confirmed the formation of ZnO NRs whilst TEM highlighted the average length and diameter in the range of 250-300 nm and 45-50 nm respectively. LB-ZnO NRs were found to be efficacious against Ae. aegypti 4th instar larvae with LC 50 of 24.74 μg/ml. Decreased acetylcholinesterase (60.6%) and glutathione S-transferase (24.4%) activities were also evaluated in Ae. aegypti larvae which were exposed to synthesized LB-ZnO NRs with no genetic aberrations. All these outcomes propose the potential employment of LB-ZnO NRs in mosquito control, as well as an effective alternative to insecticide resistance.
“…6) indicating a negative surface charge on synthesized NPs. Similar results were obtained by ZnO NRs synthesized using Santalum album leaf extract with zeta potential value of − 13 mV [68]. It should be noted that the particles with zeta potential values more positive than + 30 mV or more negative than − 30 mV are considered to be stable [69].…”
Section: Surface Charge and Stability By Zeta Potentialsupporting
Aedes aegypti mosquito species is a primary vector for dengue, chikungunya and Zika infections, and vector control is the only key approach for reducing their transmission. The present study emphasizes on environmental friendly approach for the fabrication of zinc oxide nanorods (ZnO NRs) using aqueous extract of Lumnitzera racemosa flower buds (LB) as a reducing and stabilizing agent. ZnO NRs were examined by UV-Vis spectroscopy with characteristic absorbance band at 373.82 nm and bandgap of 3.25 eV. FT-IR analysis revealed the functional groups associated with ZnO NRs. The crystallinity of LB-ZnO NRs was further investigated using X-ray diffraction. The Zeta potential displayed a surface charge of − 23.5 mV on NRs. Energy dispersive spectra analysis and SEM analysis confirmed the formation of ZnO NRs whilst TEM highlighted the average length and diameter in the range of 250-300 nm and 45-50 nm respectively. LB-ZnO NRs were found to be efficacious against Ae. aegypti 4th instar larvae with LC 50 of 24.74 μg/ml. Decreased acetylcholinesterase (60.6%) and glutathione S-transferase (24.4%) activities were also evaluated in Ae. aegypti larvae which were exposed to synthesized LB-ZnO NRs with no genetic aberrations. All these outcomes propose the potential employment of LB-ZnO NRs in mosquito control, as well as an effective alternative to insecticide resistance.
“…This further confirms that particle induced detachment of cell through activating membrane precipitating proteins. The mechanisms for ZnO nanoparticles induced toxicity may be related with oxidative stress, DNA damage and induction of apoptosis [47]. In our observation, we have determined an IC 50 of 20 lg/ml on liver cancer cells with nuclear shrinkage, nuclear damage and membrane blebbing.…”
Dengue is an arbovirus mainly vectored by Aedes mosquitoes. Its prevention and control depends to effective vector control measures. Cancer causes millions of death every year. Most of the anticancer drugs have high toxicity and low specificity of action, leading to systemic toxicity and severe side effects. Thus, the development of effective tools is a priority. We fabricated zinc oxide nanoparticles using the Myristica fragrans extract as a reducing and stabilizing agent. Nanoparticles were studied using UV-vis spectrophotometry, Fourier transform infrared spectroscopy, X-ray diffraction, zeta potential, dynamic light scattering, energy dispersive X-ray analysis, field emission scanning electron microscopy and transmission electron microscopy. ZnO nanorods were highly effective against A. aegypti young instars, with LC 50 ranging from 3.44 (larva I) to 14.63 ppm (pupa). Nanorods showed adult LC 50 of 15.004 ppm. ZnO nanorods exhibited dose-dependent cytotoxicity against human hepato-cancer cells (HepG2). After 48 and 24 h of incubation, the IC 50 were 20 and 22 lg/ml, respectively. Nanorods triggered the induction of apoptosis. Overall, this study highlights that the possibility to employ M. fragrans-synthesized ZnO nanorods in mosquito control, as well as in the development of novel chemotherapeutic agents with reduced systemic toxicity.
“…Furthermore, same stain was noted that the Aspergillus niger against human hepatocellular carcinoma cells (HepG2) cell line [ 37 ]. When Similar, AO/EtBr staining was finding in santalum album against MCF-7 [ 42 ]. Fig.…”
In around the world, mosquito control is considered a most important because of the incapable of synthetic insecticides and the ecological pollution about by them. In this manner, need the eco-friendly insecticides to efficient control the mosquito disease is the need of the hour. We synthesized the eco-friendly of zinc oxide nanoparticles (ZnO-NPs) using the
Knoxia sumatrensis
aqueous leaf extract (
Ks-
ALE) as a reducing and stabilizing agent. The synthesis of ZnO-NPs was confirmed by UV with an absorption peak at 354 nm. ZnO-NPs crystal structure was analyzed by X-ray diffraction (XRD). Fourier transform infrared spectroscopy (FT-IR) spectra revealed the chloride, cyclic alcohols, sulfonamies, carboxylic acids, oximes, phosphines, alkenes and alcohol & phenol. Field emission-scanning electron microscopy (FE-SEM) showed that the NP’s are rod shaped with 50–80 nm size and also energy dispersive spectra (EDaX) spectra showed presence of zinc. Antioxidant assay showed superior activity and evidenced by DPPH, ABTS and H
2
O
2
radical assays. Furthermore, the ZnO-NPs exhibited strong activity in MCF-7 cell line with IC
50
value is 58.87 μg/mL. Mosquito larvicidal activity of ZnO-NPs produced significant activity and excellent larvicidal activity was noticed in
Cx. quinquefasciatus
with LC
50
0.08, mg/mL and LC
90
19.46 mg/mL. This study suggests that synthesized ZnO-NPs using
Knoxia sumatrensis
leaf extract have good biological activities and it makes them an ideal candidate for pharmacological studies.
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