Induction of Osteogenic Differentiation of Adipose Derived Stem Cells by Microstructured Nitinol Actuator-Mediated Mechanical Stress

Strauß, Sarah; Dudziak, S.; Hagemann, Ronny; Barcikowski, Stephan; Fliess, Malte; Israelowitz, Meir; Kracht, Dietmar; Kuhbier, Joern W.; Radtke, Christine; Reimers, Kerstin; Vogt, Peter M.

doi:10.1371/journal.pone.0051264

Cited by 27 publications

(17 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, the biophysical induction of hASC differentiation has been reported by several authors but only in the presence of an induction medium, suggesting that mechanical loading might only boost differentiation. Nevertheless, the data from the present study are in line with a recent study demonstrating that mechanical stress alone (mediated by a microstructured nitinol actuator) can induce osteogenic differentiation (Strauß et al ., ). Some authors have reported that in in vivo animal models ESWs activated cell proliferation and bone regeneration of segmental defects (Wang et al ., ) and that the ESW‐promoted healing was achieved by stimulation of mesenchymal stem cell recruitment and differentiation into bone‐forming cells (Chen et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced differentiation of adipose-derived stem cells into osteoblast-like cells

Catalano

Marano

Rinella

et al. 2014

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced differentiation of adipose-derived stem cells into osteoblast-like cells

Catalano

Marano

Rinella

et al. 2014

J Tissue Eng Regen Med

View full text Add to dashboard Cite

“…These characteristics make NiTi alloys particularly suitable, e.g., as stent material [116–118] and scaffolds in bone tissue engineering [119]. Synthesis of NiTi nanoparticles by laser ablation in liquid has been described in the literature [115,120] and these nanoparticles were frequently adsorbed to implant surfaces as nanocoatings [121].…”

Section: Reviewmentioning

confidence: 99%

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

Rehbock¹,

Jakobi²,

Gamrad³

et al. 2014

Beilstein J. Nanotechnol.

Self Cite

136

144

View full text Add to dashboard Cite

SummaryDue to the abundance of nanomaterials in medical devices and everyday products, toxicological effects related to nanoparticles released from these materials, e.g., by mechanical wear, are a growing matter of concern. Unfortunately, appropriate nanoparticles required for systematic toxicological evaluation of these materials are still lacking. Here, the ubiquitous presence of surface ligands, remaining from chemical synthesis are a major drawback as these organic residues may cause cross-contaminations in toxicological studies. Nanoparticles synthesized by pulsed laser ablation in liquid are a promising alternative as this synthesis route provides totally ligand-free nanoparticles. The first part of this article reviews recent methods that allow the size control of laser-fabricated nanoparticles, focusing on laser post irradiation, delayed bioconjugation and in situ size quenching by low salinity electrolytes. Subsequent or parallel applications of these methods enable precise tuning of the particle diameters in a regime from 4–400 nm without utilization of any artificial surface ligands. The second paragraph of this article highlights the recent progress concerning the synthesis of composition controlled alloy nanoparticles by laser ablation in liquids. Here, binary and ternary alloy nanoparticles with totally homogeneous elemental distribution could be fabricated and the composition of these particles closely resembled bulk implant material. Finally, the model AuAg was used to systematically evaluate composition related toxicological effects of alloy nanoparticles. Here Ag+ ion release is identified as the most probable mechanism of toxicity when recent toxicological studies with gametes, mammalian cells and bacteria are considered.

show abstract

“…Most studies report that mechanical stress promotes osteogenic differentiation of BMSCs or osteoblasts (14)(15)(16)(17)(18)(19)(20)(21). Mechanical stress might also up-regulate the expression of osteogenic genes of ASC (22)(23)(24)(25). Results from our previous study have shown that cyclic tensile mechanical loading of long duration could promote the expression of BMP-2 and Runx2 and thus, osteogenic differentiation of ASCs in osteogenic medium (26) or adipogenic medium (27).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Effects of Mechanical Stretching on Osteogenic Potential of ASCs and BMSCs

et al. 2013

View full text Add to dashboard Cite

Mechanical forces play critical roles in the development and remodeling processes of bone. As an alternative cell source for bone engineering, adipose-derived stem cells (ASCs) should be fully investigated for their responses to mechanical stress. Similarly, the osteogenic potential, stimulated by mechanical stress, should be compared with bone marrow stromal cells (BMSCs), which have been clinically used for bone tissue engineering. In this study, ASCs and BMSCs were osteogenic-induced for 48 hours, and then subjected to uniaxial mechanical stretching for 2 or 6 hours. Cell orientation, osteogenic regulatory genes, osteogenic genes and ALP activities were measured and compared between ASCs and BMSCs. ASCs could align in a perpendicular way to the direction of stretching stress, while BMSCs did not present a specific alignment. Both 2 and 6 hours mechanical stretching could enhance the mRNA expression of Osx and Runx2 in BMSCs and ASCs, while OCN mRNA only increased in ASCs after 6 hours mechanical loading. Mechanical stretching enhanced the BMP-2 mRNA expression in ASCs, while only after 6 hours of mechanical loading significantly increased the BMP-2 gene expression in BMSCs. Significant differences only exist between ASCs and BMSCs loaded at 2 hours of mechanical stretching. It is concluded that ASCs are more rapid responders to mechanical stress, and have greater potential than BMSCs in osteogenesis when stimulated by mechanical stretching, indicating their usefulness for bone study in a rat model. Keywords: mechanotransduction; stem cells; osteoblasts; adipocytes Bone Research (2013) 3: 282-290. doi: 10.4248/BR201303006 IntroductionBone mesenchymal stem cells (BMSCs) and adiposederived stem cells (ASCs) have both been used experimentally for gene therapy and tissue engineering applications ( 1-2). Currently, BMSCs are obtained by aspiration of 10-40 mL of bone marrow from the iliac crest, or at the time of bone marrow biopsy, and isolated by their adherence properties (3). ASCs can be obtained from subcutaneous adipose tissue by liposuction surgeries, and these procedures yield anywhere from 100 mL to 3L †These authors contributed equally to this work. Mechanical force is one of the fundamental biological factors that affect fracture healing and bone remodeling. Most studies report that mechanical stress promotes osteogenic differentiation of BMSCs or osteoblasts (14-21). Mechanical stress might also up-regulate the expression of osteogenic genes of ASC (22-25). Results from our previous study have shown that cyclic tensile mechanical loading of long duration could promote the expression of BMP-2 and Runx2 and thus, osteogenic differentiation of ASCs in osteogenic medium (26) or adipogenic medium (27). However, limited studies were found concerning a comparison of osteogenic potential, stimulated by mechanical stress between ASCs and BMSCs. Regarding the whole bone regeneration process being affected by mechanical stress, we determined that a comparison of osteogenic differentiation by mechanical stretching ...

show abstract

Induction of Osteogenic Differentiation of Adipose Derived Stem Cells by Microstructured Nitinol Actuator-Mediated Mechanical Stress

Cited by 27 publications

References 42 publications

Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced differentiation of adipose-derived stem cells into osteoblast-like cells

Extracorporeal shockwaves (ESWs) enhance the osteogenic medium-induced differentiation of adipose-derived stem cells into osteoblast-like cells

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

Comparison of Effects of Mechanical Stretching on Osteogenic Potential of ASCs and BMSCs

Contact Info

Product

Resources

About