Induction of the adenoma-carcinoma progression and Cdc25A-B phosphatases by the trefoil factor TFF1 in human colon epithelial cells

Rodrigues, Sylvie; Rodrigue, Cm; Attoub, Samir; Jf, Fléjou; Bruyneel, Erik; Bracke, Marc; Emami, Shahin; Gespach, Christian

doi:10.1038/sj.onc.1209665

Cited by 32 publications

(21 citation statements)

References 41 publications

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“…This correlates with our data trend showing TFF1 expression is higher in SSAs, which are mostly found in the right colon, than in HP found mostly in the left, and implicates TFF1 as having a role in the progression from SSA to CRC. This is further supported by studies which show TFF1 expression progresses from absent in normal colon tissue to strong expression in premalignant cells and present but varied expression in adenocarcinomas of the colon [53,61]. In a recent study, premalignant cells transduced to force the expression of TFF1 were injected into mice.…”

Section: Discussionmentioning

confidence: 61%

Over-expression of cathepsin E and trefoil factor 1 in sessile serrated adenomas of the colorectum identified by gene expression analysis

et al. 2009

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Sessile serrated adenomas are now recognised as precursor lesions of a substantial subset of colorectal cancers arising via a so-called "serrated pathway". However, their biological markers remain to be defined. The aim of our study was to identify differentially expressed genes in sessile serrated adenomas and conventional adenomas. Gene expression analysis demonstrated molecular differences between polyp types. Further studies using quantitative real-time polymerase chain reaction on cathepsin E (CTSE) demonstrated a significantly (p < 0.05) higher expression in sessile serrated adenomas as compared to hyperplastic polyp and tubular adenomas. Trefoil Factor 1 showed the same trend of expression for sessile serrated adenomas as compared to hyperplastic polyps and was significantly higher in both polyps compared to tubular adenomas. Immunohistochemistry for both proteins demonstrated strong cytoplasmic staining of abnormal crypts in all sessile serrated adenomas, while staining in tubular adenomas and hyperplastic polyps was absent or weak and focal. BRAF and KRAS mutation analysis were employed to further validate polyp discrimination. The findings demonstrated the positive association of the BRAF mutation, V600E, with sessile serrated adenomas and KRAS mutations with tubular adenomas (p < 0.05). This study demonstrates the over-expression in CTSE, in particular, and TFF1 in sessile serrated adenomas compared to both hyperplastic polyps and tubular adenomas.

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Section: Discussionmentioning

confidence: 61%

Over-expression of cathepsin E and trefoil factor 1 in sessile serrated adenomas of the colorectum identified by gene expression analysis

et al. 2009

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“…Moreover, the BIK protein heterodimerizes with BCL2 to antagonize its antiapoptotic function (26 ). Additionally, the expression level of TFF1, a protein involved in cell protection against several forms of apoptosis (27 ), was much higher in CTC-MCC-41 than in HT-29 cells. Thus, understanding apoptosis regulation in CTCs with stem cell properties should be critical for the success of therapies targeting these cells.…”

Section: Discussionmentioning

confidence: 99%

Molecular Portrait of Metastasis-Competent Circulating Tumor Cells in Colon Cancer Reveals the Crucial Role of Genes Regulating Energy Metabolism and DNA Repair

et al. 2017

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“…The CDC25 gene can express CDC25 phosphatase protein, a cell cycle regulatory protein which serves a role in the normal cell cycle (33). In a series of human malignancies including hepatocellular carcinoma, ovarian cancer, colorectal cancer, esophageal cancer and non-Hodgkin's lymphoma, CDC25a exhibits increased expression levels (17)(18)(19)(20)(21). CDC25a exhibits pro-cancer traits in two distinct manners.…”

Section: Discussionmentioning

confidence: 99%

“…Prior investigation has revealed that cell division cycle 25A (CDC25a) is the target gene of let-7c (16). As a cell cycle regulatory protein, the abnormal expression of CDC25a is associated with the occurrence and development of multiple tumors (17)(18)(19)(20)(21). In the present study, the biological functions of let-7c in the development and progression of OC were investigated.…”

Section: Introductionmentioning

confidence: 99%

Effects of let‑7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression

et al. 2018

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Abstract. MicroRNAs serve a role in the development of ovarian cancer (OC). The present study investigated whether let-7c is able to regulate the proliferation of OC cells by targeting cell division cycle 25A (CDC25a). The reverse transcription-quantitative polymerase chain reaction was performed to detect the expression of let-7c in OC specimens. Let-7c agomir was transfected into OC cells, and the proliferation and apoptosis of OC cells were detected. A dual-luciferase assay and western blotting were performed to analyze whether CDC25a was the target gene of let-7c as well as its interaction site. The results revealed that, in OC tissue, let-7c was downregulated when compared with normal ovarian tissue. A Cell Counting Kit-8 (CCK8) assay, colony formation assay and flow cytometry demonstrated that increased expression of let-7c was able to inhibit the proliferation and increase the apoptosis of OC cells. Western blotting revealed that upregulated let-7c is able to decrease the expression of CDC25a, and a dual-luciferase assay and a recovery assay demonstrated that let-7c was able to regulate the expression of the 3' untranslated region of CDC25a. Therefore, the roles of let-7c in inhibiting the proliferation and promoting the apoptosis of OC cells may be realized through the regulation of the expression of CDC25a. The results of the present study revealed that let-7c may be a novel target in the diagnosis and treatment of OC.

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Induction of the adenoma-carcinoma progression and Cdc25A-B phosphatases by the trefoil factor TFF1 in human colon epithelial cells

Cited by 32 publications

References 41 publications

Over-expression of cathepsin E and trefoil factor 1 in sessile serrated adenomas of the colorectum identified by gene expression analysis

Over-expression of cathepsin E and trefoil factor 1 in sessile serrated adenomas of the colorectum identified by gene expression analysis

Molecular Portrait of Metastasis-Competent Circulating Tumor Cells in Colon Cancer Reveals the Crucial Role of Genes Regulating Energy Metabolism and DNA Repair

Effects of let‑7c on the proliferation of ovarian carcinoma cells by targeted regulation of CDC25a gene expression

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