Induction of β1 Integrin Synthesis by Recombinant Platelet-Derived Growth Factor (PDGF-AB) Correlates with an Enhanced Migratory Response of Human Dermal Fibroblasts to Various Extracellular Matrix Proteins

Kirchberg, K.; Lange, Thilo S.; Klein, E.; Jungtäubl, Helmut; Heinen, G.; Meyer-Ingold, Wolfgang; Scharffetter‐­Kochanek, Karin

doi:10.1006/excr.1995.1288

Cited by 48 publications

(22 citation statements)

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“…Taken together, our results suggest that this cytokine plays a role in tissue remodeling. However, there is some possibility that in vivo interaction between fibroblasts and other ECM proteins such as β1 integrin, which is reported to be upregulated by PDGF‐AB, may be essential for the upregulation of type I collagen or FN by PDGF‐CC (Kirchberg et al, 1995). Further studies are needed to clarify the clinical significance of PDGF‐C in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Regulation of fibrogenic/fibrolytic genes by platelet‐derived growth factor C, a novel growth factor, in human dermal fibroblasts

Jinnin

Ihn

Mimura

et al. 2004

Journal Cellular Physiology

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Platelet-derived growth factor (PDGF) is a potent mitogenic and chemotactic cytokine, and PDGF-C is a novel growth factor belonging to the PDGF family. In this study, to determine whether this growth factor can contribute to fibrosis and tissue remodeling, we examined the effect of PDGF-CC on the expression of fibrogenic/fibrolytic genes such as type I collagen, fibronectin (FN), matrix metalloproteinases (MMPs), and their inhibitors (TIMPs) in normal human dermal fibroblasts in vitro. PDGF elevated the levels of MMP-1 or TIMP-1 protein as well as mRNA, whereas this cytokine had no influence on the expression of type I collagen, FN, or TIMP-2. PDGF-CC also increased the levels of MMP-1 catalytic activity in the cultured media and mRNA expression, which was paralleled that on the levels of promoter activation. Additionally, PDGF-CC induced the mitogenic and migratory activity of human dermal fibroblasts in a dose-dependent manner. On the other hand, we also determined the specificity of the inhibitory effect of monoclonal antibodies against PDGF-CC generated by immunizing balb/c mice with recombinant human PDGF-CC. This antibody could inhibit the regulatory effects of MMP-1 or TIMP-1 synthesis as well as the mitogenic effects on human dermal fibroblasts induced by PDGF-CC, whereas this antibody did not affect those induced by other PDGF forms such as PDGF-AA, -AB, or -BB. These results suggest that this cytokine plays a role in the tissue remodeling.

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Section: Discussionmentioning

confidence: 99%

Regulation of fibrogenic/fibrolytic genes by platelet‐derived growth factor C, a novel growth factor, in human dermal fibroblasts

Jinnin

Ihn

Mimura

et al. 2004

Journal Cellular Physiology

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“…PDGF-AB plays an important role in the angiogenesis and cell development [125, 126]. The application of PDGF-AB in the peripheral part of the menisci showed a better healing response than the application in the central part [127].…”

Section: Growth Factorsmentioning

confidence: 99%

Biological Strategies to Enhance Healing of the Avascular Area of the Meniscus

Longo

Campi

Romeo

et al. 2012

Stem Cells International

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Meniscal injuries in the vascularized peripheral part of the meniscus have a better healing potential than tears in the central avascular zone because meniscal healing principally depends on its vascular supply. Several biological strategies have been proposed to enhance healing of the avascular area of the meniscus: abrasion therapy, fibrin clot, organ culture, cell therapy, and applications of growth factors. However, data are too heterogeneous to achieve definitive conclusions on the use of these techniques for routine management of meniscal lesions. Although most preclinical and clinical studies are very promising, they are still at an experimental stage. More prospective randomised controlled trials are needed to compare the different techniques for clinical results, applicability, and cost-effectiveness.

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“…These factors included EGF (Adelmann-Grill et al, 1990), FGF (Thomas, 1987), IL-4 (Postlethwaite and Seyer, 1991), IL-1 (Heckmann et al, 1993), PDGF-AA (Siegbahn et al, 1990), PDGF-AB (Kirchberg et al, 1995), and TGF␤ 1 (Postlethwaite et al, 1987), although antimotility effect of TGF␤ 1 was also reported (Ellis et al, 1992). None of these agents, either alone or in combination, was able to generate Ͼ30% of human serum's effect on HDF migration.…”

Section: Pdgf-bb Is the Major Promotility Factor In Human Serum For Hdfsmentioning

confidence: 99%

Mechanism of Human Dermal Fibroblast Migration Driven by Type I Collagen and Platelet-derived Growth Factor-BB

Fan

Chen

et al. 2004

MBoC

159

147

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Migration of human dermal fibroblasts (HDFs) is critical for skin wound healing. The mechanism remains unclear. We report here that platelet-derived growth factor-BB (PDGF-BB) is the major promotility factor in human serum for HDF motility on type I collagen. PDGF-BB recapitulates the full promotility activity of human serum and anti-PDGF neutralizing antibodies completely block it. Although collagen matrix initiates HDF migration without growth factors, PDGF-BB-stimulated migration depends upon attachment of the cells to a collagen matrix. The PDGF-BB's role is to provide directionality and further enhancement for the collagen-initiated HDF motility. To study the collagen and PDGF-BB "dual signaling" in primary HDF, we establish "gene cassettes" plus lentiviral gene delivery approach, in which groups of genes are studied individually or in combination for their roles in HDF migration. Focal adhesion kinase, p21Rac,CDC42 -activated kinase and Akt are grouped into an upstream kinase gene cassette, and the four major mitogenactivated protein kinases (extracellular signal-regulated kinase 1/2, p38, c-Jun NH 2 -terminal kinase, and extracellular signal-regulated kinase 5) are grouped into a downstream kinase gene cassette. The experiments demonstrate 1) the genes' individual roles and specificities, 2) their combined effects and sufficiency, and 3) the mechanisms of their intermolecular connections in HDF migration driven by collagen and PDGF-BB.

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Induction of β1 Integrin Synthesis by Recombinant Platelet-Derived Growth Factor (PDGF-AB) Correlates with an Enhanced Migratory Response of Human Dermal Fibroblasts to Various Extracellular Matrix Proteins

Cited by 48 publications

References 0 publications

Regulation of fibrogenic/fibrolytic genes by platelet‐derived growth factor C, a novel growth factor, in human dermal fibroblasts

Regulation of fibrogenic/fibrolytic genes by platelet‐derived growth factor C, a novel growth factor, in human dermal fibroblasts

Biological Strategies to Enhance Healing of the Avascular Area of the Meniscus

Mechanism of Human Dermal Fibroblast Migration Driven by Type I Collagen and Platelet-derived Growth Factor-BB

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