INDUKSI KALUS DAN TUNAS DARI EKSPLAN PUCUK BROKOLI (Brassica oleracea  L. sub var. italica Planch) PADA MEDIUM MS YANG DIBERIKAN NAA DAN BAP

Tilaar, Wenny; Tulung, Stella

doi:10.35791/eug.19.1.2013.8377

Cited by 3 publications

(3 citation statements)

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“…Teak plant (Tectonagrandis) nodal explants treated with 4 mg/l BAP produced the maximum number of shoots (2,375) per explant [3].In the Brassica oleaceae L study, the optimal number of shoots was produced by a BAP treatment of 5 mg/l and 0.1 mg/l NAA. On average, 2.66 shoots were produced [6]. This demonstrated that MS medium combined with the growth regulator BAP can produce shoots.…”

Section: Introductionmentioning

confidence: 79%

The effects of several concentrations of BAP and source of explants to gambier shoot induction (Uncaria gambier (Hunter) Roxb)

Zainal

Anwar

Gustian

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Gambir is a type of plantation plant that has many benefits. Gambir extract can be used for the food, cosmetic, pharmaceutical, and leather tanning industries. However, the availability of gambier has decreased in production. Hence, information and technology for propagating Gambir seeds in vitro is needed. The growth of plant explants can be impacted by the application of growth regulators and explant sources in-vitro. This study’s purpose was to ascertain the connection between BAP concentration and explant sources concerning the development of gambier shoots. In addition, it is also to obtain of BAP and the best source of explants for the induction of the gambier shoots. In this study, a Factorial Completely Randomized Design (CRD) with 6 treatments that were each repeated five times was used. There are two levels of explant source and three levels of BAP concentration, the first of which is the concentration of BAP (2 mg/l, 4 mg/l, and 6 mg/l) (the first node and the third node from the shoot). The findings demonstrated that, in general, 100% of shoots could form when BAP and explant sources were concentrated. The concentration of BAP and the origin of the explants significantly influenced the emergence time of the shoots, with the quickest time of budding occurring after 3.8 days at a concentration of 2 mg/l BAP at the third node. 6 mg/l BAP was the optimal treatment for increasing the number of gambier leaves and shoots.

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Section: Introductionmentioning

confidence: 79%

The effects of several concentrations of BAP and source of explants to gambier shoot induction (Uncaria gambier (Hunter) Roxb)

Zainal

Anwar

Gustian

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Penelitian Nurwahyuni (2013) mengenai perbanyakan Citrus nobilis Brastepu menggunakan eksplan pucuk diperoleh bahwa konsentrasi 0,5 mg/L BAP efektif dalam memulai pertumbuhan pucuk. Penelitian lain oleh Tilaar & Tulung (2013) tentang kultur pucuk juga telah dilakukan pada tanaman brokoli. Kombinasi optimal untuk tinggi tunas dalam penelitian ini adalah 1 ppm NAA dengan 3 ppm BAP, sementara kombinasi terbaik untuk jumlah tunas adalah 0,1 ppm NAA dengan 5 ppm BAP.…”

Section: Pendahuluanunclassified

In Vitro Shoot Culture Growth of Pontianak Orange (Citrus nobilis var. microcarpa) with the Addition of Naphthalene Acetic Acid and 6-Benzyl Amino Purine

Nissa,

Zakiah,

Turnip

2023

JBT

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Pontianak siam orange (Citrus nobilis var. microcarpa) is an important agricultural commodity unique to Sambas Regency, West Kalimantan. The demand for Pontianak siam oranges has been steadily increasing over the years, yet the production of Pontianak oranges has not been able to meet the domestic demand. The limited availability of superior seedlings is one of the reasons for this situation. Therefore, efforts are needed to fulfill the demand for Pontianak siam oranges by producing a large quantity of superior seedlings in a short period. One of the strategies that can be employed is in vitro propagation. This research aims to observe the growth of Pontianak Siamese orange shoot explants after administration of NAA (Naphthalene Acetic Acid) and BAP (6-Benzyl Amino Purine). This research was conducted from January to June 2023 at the Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Tanjungpura University. The study employed a Completely Randomized Design (CRD) with two treatment factors namely NAA concentrations with 4 levels (0; 0.25; 0.5; 0.75 mg/L), and BAP concentrations with 5 levels (0; 0.5; 1; 1.5; 2 mg/L). Parameters observed were the emergence time of shoot, shoot number, height of shoot, and the number of leaves. The results showed that single NAA, single BAP, and combination treatment of NAA and BAP significantly affected all growth parameters. Treatment with the addition of 0.25 mg/L NAA and 1 mg/L BAP (A1B2) was the treatment with the fastest shoot emergence time (2.80 days after planting), the largest number of shoots (2.60 shoots)the tallest shoots (1.50 cm), , and the greatest number of leaves (2.60 leaves).

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“…BAP adalah sitokinin yang sering digunakan karena paling efektif untuk merangsang pembentukan tunas, lebih stabil dan tahan terhadap oksidasi serta paling murah diantara sitokinin lainnya (Nurjanah, 2009). Penelitian yang dilakukan oleh (Tilaar & Rantung, 2013) menjelaskan bahwa BAP 2 ppm mampu mempercepat waktu bertunas.…”

Section: Pendahuluanunclassified

PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

.¹,

Polii-Mandang²,

Tilaar³

2018

AGRSOSEK

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This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

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INDUKSI KALUS DAN TUNAS DARI EKSPLAN PUCUK BROKOLI (Brassica oleracea L. sub var. italica Planch) PADA MEDIUM MS YANG DIBERIKAN NAA DAN BAP

Cited by 3 publications

References 1 publication

The effects of several concentrations of BAP and source of explants to gambier shoot induction (Uncaria gambier (Hunter) Roxb)

The effects of several concentrations of BAP and source of explants to gambier shoot induction (Uncaria gambier (Hunter) Roxb)

In Vitro Shoot Culture Growth of Pontianak Orange (Citrus nobilis var. microcarpa) with the Addition of Naphthalene Acetic Acid and 6-Benzyl Amino Purine

PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

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