Industrial vitamin B12 production by Pseudomonas denitrificans using maltose syrup and corn steep liquor as the cost-effective fermentation substrates

Xia, Wei; Chen, Wei; Peng, Wei-fu; Li, Kun-tai

doi:10.1007/s00449-014-1348-5

Cited by 40 publications

(20 citation statements)

References 21 publications

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“…Despite the fact that betaine delays cell growth, betaine feeding during fermentation has been revealed as an effective strategy to increase vitamin B 12 production [ 101 ]. To reduce medium and fermentation costs, cheap carbon sources such as maltose syrup and corn steep liquor can be used to replace refined sucrose [ 102 ].…”

Section: Other Strategies To Improve Cobalamin Productionmentioning

confidence: 99%

Microbial production of vitamin B12: a review and future perspectives

2017

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Vitamin B12 is an essential vitamin that is widely used in medical and food industries. Vitamin B12 biosynthesis is confined to few bacteria and archaea, and as such its production relies on microbial fermentation. Rational strain engineering is dependent on efficient genetic tools and a detailed knowledge of metabolic pathways, regulation of which can be applied to improve product yield. Recent advances in synthetic biology and metabolic engineering have been used to efficiently construct many microbial chemical factories. Many published reviews have probed the vitamin B12 biosynthetic pathway. To maximize the potential of microbes for vitamin B12 production, new strategies and tools are required. In this review, we provide a comprehensive understanding of advances in the microbial production of vitamin B12, with a particular focus on establishing a heterologous host for the vitamin B12 production, as well as on strategies and tools that have been applied to increase microbial cobalamin production. Several worthy strategies employed for other products are also included.

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Section: Other Strategies To Improve Cobalamin Productionmentioning

confidence: 99%

Microbial production of vitamin B12: a review and future perspectives

2017

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“…B 12 is not found in any food products of plant origin [34]. For pharmaceutical purposes, B 12 is isolated as a byproduct derived from the following microorganisms: Propionibacterium freudenreichii, Propionibacterium shermanii and Pseudomonas denitrificans [51].…”

Section: Sources Of Vitamin B 12mentioning

confidence: 99%

Understanding vitamin B12

Hüpsch-Marzec¹,

Pawlak²,

Skaba³

2019

jos

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Dentists often act as primary care physicians. If they are able to identify symptoms of vitamin B 12 deficiency, dentists can identify the problem at the early stage of the pathology. They can make appropriate referrals to specialists who can make a diagnosis and recommend treatment. Effective treatment may be possible at the stage before neurological symptoms occur, preventing irreversible changes to the body. Vitamin B 12 (cobalamin) plays an essential role in many metabolic processes, including biosynthesis of nucleic acids. The clinical manifestations of vitamin B 12 deficiency are highly polymorphic and depend on many factors. This review presents fundamental information about the structure of vitamin B 12 , its dietary sources, up-to-date understanding of its role in the human body and the most important consequences of its deficiency, including manifestations within the mucous membrane of the oral cavity. Due to the fact that a large percentage of adults and elderly patients have an undiagnosed vitamin B 12 deficiency it is prudent to screen for vitamin B 12 status. Methylmalonic acid and/or holotranscobalamin II (holoTCII) have been proposed to be the most sensitive assessment methods. Among high risk groups (e.g. the elderly, vegetarians, people with gastrointestinal health conditions), screening should be recommended at each annual examination.

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“…Nitrogen also plays an important role in microbial fermentation, and a cheap and simple organic nitrogen compound is preferable to expensive and complex nitrogen sources, such as yeast extract and beef extract [24]. Corn steep liquor (CSL), a by-product of the starch industry, is an inexpensive nitrogen source that can be used for cultivation of microorganisms, because it contains a rich complement of nitrogenous compounds, vitamins, amino acids, and biotins [25,26].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

Wang

Heo

et al. 2020

Microb Cell Fact

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Background: 1,3-propanediol (1,3-PDO) is the most widely studied value-added product that can be produced by feeding glycerol to bacteria, including Lactobacillus sp. However, previous research reported that L. reuteri only produced small amounts and had low productivity of 1,3-PDO. It is urgent to develop procedures that improve the production and productivity of 1,3-PDO. Results:We identified a novel L. reuteri CH53 isolate that efficiently converted glycerol into 1,3-PDO, and performed batch co-fermentation with glycerol and glucose to evaluate its production of 1,3-PDO and other products. We optimized the fermentation conditions and nitrogen sources to increase the productivity. Fed-batch fermentation using corn steep liquor (CSL) as a replacement for beef extract led to 1,3-PDO production (68.32 ± 0.84 g/L) and productivity (1.27 ± 0.02 g/L/h) at optimized conditions (unaerated and 100 rpm). When CSL was used as an alternative nitrogen source, the activity of the vitamin B12-dependent glycerol dehydratase (dhaB) and 1,3-propanediol oxidoreductase (dhaT) increased. Also, the productivity and yield of 1,3-PDO increased as well. These results showed the highest productivity in Lactobacillus species. In addition, hurdle to 1,3-PDO production in this strain were identified via analysis of the half-maximal inhibitory concentration for growth (IC50) of numerous substrates and metabolites. Conclusions:We used CSL as a low-cost nitrogen source to replace beef extract for 1,3-PDO production in L. reuteri CH53. These cells efficiently utilized crude glycerol and CSL to produce 1,3-PDO. This strain has great promise for the production of 1,3-PDO because it is generally recognized as safe (GRAS) and non-pathogenic. Also, this strain has high productivity and high conversion yield.

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Industrial vitamin B12 production by Pseudomonas denitrificans using maltose syrup and corn steep liquor as the cost-effective fermentation substrates

Cited by 40 publications

References 21 publications

Microbial production of vitamin B12: a review and future perspectives

Microbial production of vitamin B12: a review and future perspectives

Understanding vitamin B12

Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53

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