Infection of BALB/c A mice by spiral and coccoid forms of Helicobacter pylori

Wang, X; Sturegård, Erik; Rupar, R; Nilsson, Hans‐Olof; Aleljung, Pär; Carlén, Birgitta; Wïllén, Roger; Wadström, Torkel

doi:10.1099/00222615-46-8-657

Cited by 78 publications

(68 citation statements)

References 25 publications

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“…The H. pylori mouse-passaged strain 119/95 (a CagAπ, VacAπ strain originally isolated from a duodenal ulcer patient at the University Hospital of Lund, Sweden) (13) was grown on GAB-Camp agar supplemented with 10% horse serum and incubated for 48 h at 37aeC under microaerophilic conditions (14). The cells were harvested in PBS, centrifuged at 3000 rpm for 10 min, and resuspended in PBS to a final concentration of 10 9 colony-forming units (cfu)/ ml.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Development of high-grade lymphoma in Helicobacter pylori -infected C57BL/6 miceNote

Wang

Wïllén

Andersson

et al. 2000

Apmis

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Section: Bacterial Strainsmentioning

confidence: 99%

Development of high-grade lymphoma in Helicobacter pylori -infected C57BL/6 miceNote

Wang

Wïllén

Andersson

et al. 2000

Apmis

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“…More recent reports have been published on the colonization of immuno-competent mice with mouse-adapted H. pylori isolates (Ferrero et al, 1995;Marchetti et al, 1995;Ayraud et al, 2002) with fresh clinical H. pylori isolates (Marchetti et al, 1995;Lee et al, 1997;Ayraud et al, 2002;Guo and Mekalanos, 2002) and coccoid forms of H. pylori (Cellini et al, 1994;Aleljung et al, 1996;Wang et al, 1997;Hua et al, 1998;Rabelo-Gonçalves et al, 2002;She et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria

2005

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An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10 8 CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori induced lymphomonocytic infiltration that was present from days 7 to 60 in group GA. In group GB, it was only present from days 14 to 35. In conclusion, our data suggested that freezing altered pathogenic properties of H. pylori and probably inhibited expression of bacterial antigens and consequently the establishment and maintenance of infection. Although the animals developed mild duodenitis and gastritis, the BALB/c mouse is not susceptible to developing peptic ulcers during H. pylori infection.

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“…PBS was used as a negative control (9). The bacteria were cultured freshly on agar prior to infection.…”

Section: Experimental Designmentioning

confidence: 99%

“…A monkey model was used to infect animals with multiple strains of H. pylori (11). The observation that H. pylori strains can cause a varying degree of inflammation in BALB/cA mice (9) and by utilising multiple strains to infect mice, makes it possible to investigate if strains can act synergistically and become more detrimental for the host. RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) analysis has been used successfully for typing H. pylori (12,13) and found to be a good method to identify the original and re-isolated strains from infected animals.…”

Section: Introductionmentioning

confidence: 99%

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RAPD-PCR, Histopathological and Serological Analysis of Four Mouse Strains Infected with Multiple Strains ofHelicobacter pylori

Wang

1999

Microbial Ecology in Health and Disease

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The aims of this study were to investigate: i) if a specific strain(s) became predominant in the stomach of mice infected with multiple strains of freshly isolated H. pylori; ii) if a time dependent selection of strains occurs; iii) to compare the degree of gastric inflammation in C57BL/6, CBA/Ca, BALB/cA and NMRI mice; and iv) to follow the serological response to the infection. Six weeks old mice were inoculated orally 3 times at 2-day intervals with 9 freshly isolated H. pylori strains. Five animals of each mouse strain were sacrificed at 2, 4, 10 and 16 weeks post-inoculation. Stomach smears were cultured on GAB-Camp agar at 37°C for 5 -10 days under microaerophilic conditions. Seven to 10 single H. pylori colonies from each animal were subcultured and identified by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. Histopathology of the gastric mucosa and serum antibody response were also analysed for each animal.Most mice were found to be infected by 1 strain (119/95). In only a few animals were multiple strains recovered. Strain 119/95 constituted 90.5% of the 577 mouse-passaged isolates and when tested it expressed both vacuolating cytotoxin antigen (VacA) and cytotoxin associated antigen (CagA). The inflammation scores of murine stomachs and duodenum samples of infected animals were significantly higher than in non-infected control animals for all 4 mouse strains. No significant differences in inflammation score were observed for the mouse strains 16 weeks post-infection. The immune response was also higher in infected versus non-infected animals for all mouse strains. The specific serum anti H. pylori immune response was highest for the NMRI mice followed by BALB/cA, C57BL/6, and CBA/Ca mice.

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Infection of BALB/c A mice by spiral and coccoid forms of Helicobacter pylori

Cited by 78 publications

References 25 publications

Development of high-grade lymphoma in Helicobacter pylori -infected C57BL/6 miceNote

Development of high-grade lymphoma in Helicobacter pylori -infected C57BL/6 miceNote

Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria

RAPD-PCR, Histopathological and Serological Analysis of Four Mouse Strains Infected with Multiple Strains ofHelicobacter pylori

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