Infection with a pathogenic turkey coronavirus isolate negatively affects growth performance and intestinal morphology of young turkey poults in Canada

Gomaa, M. H.; Yoo, Dongwan; Ojkić, Davor; Barta, John R.

doi:10.1080/03079450903055389

Cited by 21 publications

(14 citation statements)

References 29 publications

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“…These include, both in the Americas and in Europe, type 2 turkey astroviruses, picorna-like viruses, reoviruses, rotaviruses, and adenoviruses (Andral et al, 1985;Reynolds & Saif, 1986;Gough & Drury, 1998;Guy, 1998;Koci et al, 2000;Cattoli et al, 2007;Da Silva et al, 2008;PantinJackwood et al, 2008a;Woolcock & Shivaprasad, 2008;Jindal et al, 2009b). However, experimental studies with these agents inoculated alone Heggen-Peay et al, 2002;Pantin-Jackwood et al, 2008b;Gomaa et al, 2009) or in combinations (Yu et al, 2000;Jindal et al, 2009a;Spackman et al, 2010) seldom reproduce the whole range of signs observed in the diseased flocks. Determining whether the newly detected Fr TCoV plays a significant role in the increased incidence of digestive disorders reported in French turkey flocks since 2003 (Germain & Rousseau, 2005) will require isolation of the virus and further in vivo experimental studies.…”

Section: Discussionmentioning

confidence: 99%

First full-length sequences of the S gene of European isolates reveal further diversity among turkey coronaviruses

et al. 2011

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An increasing incidence of enteric disorders clinically suggestive of the poult enteritis complex has been observed in turkeys in France since 2003. Using a newly designed real-time reverse transcriptase-polymerase chain reaction assay specific for the nucleocapsid (N) gene of infectious bronchitis virus (IBV) and turkey coronaviruses (TCoV), coronaviruses were identified in 37% of the intestinal samples collected from diseased turkey flocks. The full-length spike (S) gene of these viruses was amplified, cloned and sequenced from three samples. The French S sequences shared 98% identity at both the nucleotide and amino acid levels, whereas they were at most 65% and 60% identical with North American (NA) TCoVand at most 50% and 37% identical with IBV at the nucleotide and amino acid levels, respectively. Higher divergence with NA TCoV was observed in the S1-encoding domain. Phylogenetic analysis based on the S gene revealed that the newly detected viruses form a sublineage genetically related with, but significantly different from, NA TCoV. Additionally, the RNAdependent RNA polymerase gene and the N gene, located on the 5? and 3? sides of the S gene in the coronavirus genome, were partially sequenced. Phylogenetic analysis revealed that both the NA TCoV and French TCoV (Fr TCoV) lineages included some IBV relatives, which were however different in the two lineages. This suggested that different recombination events could have played a role in the evolution of the NA and Fr TCoV. The present results provide the first S sequence for a European TCoV. They reveal extensive genetic variation in TCoV and suggest different evolutionary pathways in North America and Europe.

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Section: Discussionmentioning

confidence: 99%

First full-length sequences of the S gene of European isolates reveal further diversity among turkey coronaviruses

et al. 2011

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“…The presence of TCoV in the filtrate was confirmed using negatively stained filtrate and transmission electron microscopy as well as RT-PCR of a portion of the nucleocapsid gene (Gomaa et al, 2009a). Extensive testing of the resulting TCoV-MG10 filtrate was done to exclude the possibility of the presence of any pathogens other than TCoV using electron microscopy as well as culture for bacteria, other viruses and mycoplasmas (Gomaa et al, 2009b).…”

Section: Virusmentioning

confidence: 99%

Use of recombinant S1 spike polypeptide to develop a TCoV-specific antibody ELISA

Gomaa

Yoo

Ojkić

et al. 2009

Veterinary Microbiology

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Turkey coronavirus (TCoV) causes diarrhoea in young turkey poults but little is known about its prevalence in the field. To address this, a portion of the S1 region of the spike glycoprotein of TCoV carrying relevant B cell epitopes (amino acid positions 54-395) was cloned and expressed in Escherichia coli. This protein was purified and used to develop an indirect ELISA for detection of antibodies against TCoV. Using experimentally derived positive and negative turkey serum samples this ELISA showed high sensitivity (95%) and specificity (92%) for TCoV. To further evaluate the potential of the ELISA, 360 serum samples from commercial turkey farms in Ontario were tested for TCoV-specific antibodies using the recombinant TCoV ELISA. High seroprevalence of TCoV was found with 71.11% of breeders and 56.67% of meat turkeys testing seropositive. Although there was significant positive correlation with a TCoV-N protein-based ELISA, there was little to no correlation with the whole IBV antigen-based ELISA when field sera were tested for antibodies against TCoV.

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“…Turkey coronavirus (TCoV) causes atrophic enteritis in turkeys and outbreaks or cases of turkey coronaviral enteritis occurred and still occurs in the USA [1], Canada [2], Brazil [3], and Europe [4]. TCoV belongs to species Avian coronavirus of the genus Gammacoronavirus in the family Coronaviridae.…”

Section: Introductionmentioning

confidence: 99%

Real-Time Reverse Transcription-Polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues

Chen

Lin

2016

Springer Protocols Handbooks

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Turkey coronavirus (TCoV) infection causes acute atrophic enteritis in turkey poults, leading to significant economic loss in the turkey industry. Rapid detection, differentiation, and quantitation of TCoV are critical to the diagnosis and control of the disease. A specific one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay using TCoV-specific primers and dual-labeled fluorescent probe for detection and quantitation of TCoV in feces and intestine tissues is described in this chapter. The fluorogenic probe labeled with a reporter dye (FAM, 6-carboxytetramethylrhodamine) and a quencher dye (Absolute Quencher™) was designed to bind to a 186 base-pair fragment flanked by the two PCR primers targeting the 3′ end of spike gene (S2) of TCoV. The assay is highly specific and sensitive and can quantitate between 10 2 and 10 10 copies/mL of viral genome. It is useful in monitoring the progression of TCoV-induced atrophic enteritis in the turkey flocks.

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Infection with a pathogenic turkey coronavirus isolate negatively affects growth performance and intestinal morphology of young turkey poults in Canada

Cited by 21 publications

References 29 publications

First full-length sequences of the S gene of European isolates reveal further diversity among turkey coronaviruses

First full-length sequences of the S gene of European isolates reveal further diversity among turkey coronaviruses

Use of recombinant S1 spike polypeptide to develop a TCoV-specific antibody ELISA

Real-Time Reverse Transcription-Polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues

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