Use of recombinant S1 spike polypeptide to develop a TCoV-specific antibody ELISA

Gomaa, M. H.; Yoo, Dongwan; Ojkić, Davor; Barta, John R.

doi:10.1016/j.vetmic.2009.04.010

Cited by 15 publications

(13 citation statements)

References 32 publications

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“…However, the seroprevalence of this virus in commercial flocks was above 64% [ 30 ]. Furthermore, a similar seroprevalence of almost 74% in breeder turkeys and 60% in meat-type animals in Canadian commercial flocks were noted [ 31 ]. TCoV was also not detected in studies of healthy and diseased turkey flocks in Turkey [ 32 ].…”

Section: Discussionmentioning

confidence: 79%

Molecular Epidemiology of Turkey Coronaviruses in Poland

Domańska-Blicharz

Lisowska

Opolska

et al. 2022

Viruses

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The only knowledge of the molecular structure of European turkey coronaviruses (TCoVs) comes from France. These viruses have a quite distinct S gene from North American isolates. The aim of the study was to estimate the prevalence of TCoV strains in a Polish turkey farm during a twelve-year period, between 2008 and 2019, and to characterize their full-length S gene. Out of the 648 flocks tested, 65 (10.0%, 95% CI: 7.9–12.6) were positive for TCoV and 16 of them were molecularly characterized. Phylogenetic analysis showed that these strains belonged to two clusters, one formed by the early isolates identified at the beginning of the TCoV monitoring (from 2009 to 2010), and the other, which was formed by more recent strains from 2014 to 2019. Our analysis of the changes observed in the deduced amino acids of the S1 protein suggests the existence of three variable regions. Moreover, although the selection pressure analysis showed that the TCoV strains were evolving under negative selection, some sites of the S1 subunit were positively selected, and most of them were located within the proposed variable regions. Our sequence analysis also showed one TCoV strain had recombined with another one in the S1 gene. The presented investigation on the molecular feature of the S gene of TCoVs circulating in the turkey population in Poland contributes interesting data to the current state of knowledge.

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Section: Discussionmentioning

confidence: 79%

Molecular Epidemiology of Turkey Coronaviruses in Poland

Domańska-Blicharz

Lisowska

Opolska

et al. 2022

Viruses

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“…Many glycoproteins from cells or viruses produced in this system have shown certain biological activities Gomaa et al, 2009;Schwanke et al, 2009;Li et al, 2010). In this study, a N-terminal signal peptide (31 amino acids in length) was identified in the protein using bioinformatics analysis.…”

Section: Discussionmentioning

confidence: 99%

Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: A way for producing functional protein in prokaryotic microorganism

Ren

Wang

Yin

et al. 2010

Journal of Biotechnology

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Based on the bioinformatics analysis of the gene encoding glycoprotein 5 (GP5) of porcine reproductive and respiratory syndrome virus (PRRSV) isolate HH08, two gene fragments were amplified by polymerase chain reaction (PCR), deleting the signal peptide and transmembrane sequences in GP5 gene. Both gene fragments were designated GP5a and GP5b, respectively. They were ligated with a linker and cloned into prokaryotic expression vector, pET-30a. Expression of the protein of interest was induced by isopropyl beta-d-1-thiogalactopyranoside. The purified protein was used as an immunogen to elicit antibody in rabbit. The immunoreactivity of the protein was determined using ELISA and Western blot. Biologically active GP5 and anti-GP5 antibody inhibited cell infection by PRRSV. Moreover, the antibody produced in this study was capable of detecting the cell infection by PRRSV and distinguishing this virus from other viruses.

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“…Some studies reported the development of two recombinant‐based ELISAs for serological diagnosis of turkey coronaviruses (TCoV) 7,149 . These techniques are based on the expression of the TCoV‐N 149 and the RBD of the TCoV‐S 150 proteins. Although both ELISAs showed great success in the detection of specific TCoV antibodies, the TCoV‐S based ELISA remained to be much more specific than the TCoV‐N‐ELISA 149,150 .…”

Section: Pitfalls Related To the Antibodies Related Assaysmentioning

confidence: 99%

The next‐generation coronavirus diagnostic techniques with particular emphasis on the SARS‐CoV‐2

Hemida

2021

Journal of Medical Virology

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The potential zoonotic coronaviruses (SARS-CoV, MERS-CoV, and SARS-CoV-2) are of global health concerns. Early diagnosis is the milestone in their mitigation, control, and eradication. Many diagnostic techniques are showing great success and have many advantages, such as the rapid turnover of the results, high accuracy, and high specificity and sensitivity. However, some of these techniques have several pitfalls if samples were not collected, processed, and transported in the standard ways and if these techniques were not practiced with extreme caution and precision. This may lead to false-negative/positive results. This may affect the downstream management of the affected cases. These techniques require regular finetuning, upgrading, and optimization. The continuous evolution of new strains and viruses belong to the coronaviruses is hampering the success of many classical techniques. There are urgent needs for next generations of coronaviruses diagnostic assays that overcome these pitfalls. This new generation of diagnostic tests should be able to do simultaneous, multiplex, and high-throughput detection of various coronavirus in one reaction. Furthermore, the development of novel assays and techniques that enable the in situ detection of the virus on the environmental samples, especially air, water, and surfaces, should be given considerable attention in the future. These approaches will have a substantial positive impact on the mitigation and eradication of coronaviruses, including the current SARS-CoV-2 pandemic.

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Use of recombinant S1 spike polypeptide to develop a TCoV-specific antibody ELISA

Cited by 15 publications

References 32 publications

Molecular Epidemiology of Turkey Coronaviruses in Poland

Molecular Epidemiology of Turkey Coronaviruses in Poland

Heterologous expression of fused genes encoding the glycoprotein 5 from PRRSV: A way for producing functional protein in prokaryotic microorganism

The next‐generation coronavirus diagnostic techniques with particular emphasis on the SARS‐CoV‐2

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