Infectivity of a Japanese isolate of Oidium neolycopersici KTP-01 to a European tomato cultivar resistant to O. lycopersici

Kashimoto, Koichi; Sameshima, Takeshi; Matsuda, Yoshinori; Nonomura, Teruo; Oichi, Wataru; Kakutani, Koji; Nakata, Kengo; Kusakari, Shin-ichi; Toyoda, Hideyoshi

doi:10.1007/s10327-003-0070-y

Cited by 36 publications

(26 citation statements)

References 12 publications

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“…The existence of pathogen races was also shown by Kashimoto et al (2003b), who recorded that Japanese isolate KTP-01 was able to infect resistant tomato cultivar Grace, originating from the Netherland.…”

Section: Intraspecific Variability Of O Neolycopersicimentioning

confidence: 76%

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Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

et al. 2013

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Tomato powdery mildew (Oidium neolycopersici) is one of the most devastating diseases of cultivated tomatoes worldwide. Although the first epidemics were recorded more than 25 years ago many aspects of this host-pathogen interaction are still not well understood. Detailed morphological and molecular studies of the anamorphs confirmed that O. neolycopersici is phylogeneticaly close to Erysiphe aquilegiae var. ranunculi. Host range is rather broad, apart from Solanaceae hosts were found in the families Apocynaceae, Campanulaceae, Crassulaceae, Cistaceae, Cucurbitaceae, Linaceae, Malvaceae, Papaveraceae, Pedialiaceae, Scrophulariaceae, Valerianaceae a Violaceae. Non-host resistance within these families is not based on inhibition of formation of primary haustorium, however, on post-haustorial hypersensitive reponse and another type of non-hypersensitive resistance. Screening of wild Solanum species (previous Lycopersicon spp.) germplasm revealed valuable sources of resistance (S. habrochaites, S. pennellii, S. cheesmaniae, S. chilense, S. peruvianum). The main resistance mechanism was found to be a hypersensitive response (HR), in some cases followed by limited development of the pathogen. However, there is a broad variation in resistance response on the histological and cytological level. Interaction between many wild Solanum spp. and O. neolycopersici is race-specific, at least three races were differentiated. In some interspecific crosses (S. lycopersicum × S. habrochaites) adult plant resistance was observed. Biochemical studies focusing on production of reactive oxygen species (ROS) and peroxidase activity during infection of O. neolycopersici showed that production of ROS and activity of corresponding enzymes is related to activation of defence responses in genotypes of wild Solanum sect. Lycopersicon. The significance of nitric oxide (NO) in O. neolycopersici pathogenesis was supported by experiments with NO donors and scavengers. In moderately resistant genotype S. chmielewskii, treatment by heat stress caused slight deceleration of pathogen development, increased production of jasmonic acid (JA) and abscisic acid (ABA) and increased peroxidase activity in infected plants. The different degree of tomato resistance/ susceptibility did not markedly change the rate and extent of photosynthetic response to O. neolycopersici; only minimal impairment of photosynthesis was found in both susceptible and moderately resistant genotypes during the Harper Adams University, Newport, Shropshire TF10 8NB, UK first 9 days after inoculation. The accumulated evidence confirm a crucial role of localised increased production of ROS and reactive nitrogen species (RNS) in response to pathogen penetration into plant tissue and its involvement in the plant resistance responses including the initiation and progression of plant cell death in host wild Solanum species. Crucial points of further research are discussed.

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Section: Intraspecific Variability Of O Neolycopersicimentioning

confidence: 76%

“…cv. "Grace"; Kashimoto et al (2003b)). Afterwards racespecificity of the interactions in this pathosystem was confirmed when different isolates of O. neolycopersici overcame the resistance of these lines Bai et al 2005).…”

Section: Host Range Of O Neolycopersicimentioning

confidence: 99%

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

et al. 2013

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“…KTP-01 was highly infectious not only to all Japanese tomato cultivars tested (Kashimoto et al 2003a) but also to a European cultivar bred for resistance to another pathotype of O. (neo)lycopersici (Kashimoto et al 2003b). The pathogen produced abundant noncatenated conidia that spread rapidly among vicinal tomato plants.…”

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Consecutive monitoring for conidiogenesis by Oidium neolycopersici on tomato leaves with a high-fidelity digital microscope

et al. 2004

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Conidiogenesis by Oidium neolycopersici KTP-01 on tomato leaves was vitally monitored with a high-fidelity digital microscope. Conidiophores were initially formed 3 days after inoculation and then elongated to a maximum length within at least 12 h. The apical part was split into two cells after two successive septations, accompanied by apical expansion. These cells subsequently developed into primary and secondary conidia. An additional septation at the stem portion of the conidiophores produced a generative and a foot cell. Subsequent conidiation occurred during repeated cycles of splitting of the generative cell, maturation of the apical cell into a conidium, and abstriction of the conidium. To our knowledge, this report is the first on the developmental process of conidiogenesis by powdery mildew on host leaves as revealed with the digital microscope.

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“…4) known to be suppressed by these resistance genes (4). Also, the Japanese isolate KTP-01, reported to infect the tomato cultivar Grace, which is resistant to some European and American O. neolycopersici populations (30), grouped together with some European and American isolates. However, this isolate was somewhat distant from the others in the first sub-group (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Other studies have lead to the same conclusion. Kashimoto et al (30) showed that the tomato cv. Grace bred in the Netherlands showing resistance to several European and United States strains of O. neolycopersici, was susceptible to a Japanese isolate, KTP-01, of the pathogen.…”

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confidence: 99%

Oidium neolycopersici: Intraspecific Variability Inferred from Amplified Fragment Length Polymorphism Analysis and Relationship with Closely Related Powdery Mildew Fungi Infecting Various Plant Species

Jankovics¹,

Bai²,

Kovács³

et al. 2008

Phytopathology®

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Jankovics, T., Bai, Y., Kovács, G. M., Bardin, M., Nicot, P. C., Toyoda, H., Matsuda, Y., Niks, R. E., and Kiss, L. 2008. Oidium neolycopersici: Intraspecific variability inferred from amplified fragment length polymorphism analysis and relationship with closely related powdery mildew fungi infecting various plant species. Phytopathology 98:529-540.Previous works indicated a considerable variation in the pathogenicity, virulence, and host range of Oidium neolycopersici isolates causing tomato powdery mildew epidemics in many parts of the world. In this study, rDNA internal transcribed spacer (ITS) sequences, and amplified fragment length polymorphism (AFLP) patterns were analyzed in 17 O. neolycopersici samples collected in Europe, North America, and Japan, including those which overcame some of the tomato major resistance genes. The ITS sequences were identical in all 10 samples tested and were also identical to ITS sequences of eight previously studied O. neolycopersici specimens. The AFLP analysis revealed a high genetic diversity in O. neolycopersici and indicated that all 17 samples represented different genotypes. This might suggest the existence of either a yet unrevealed sexual reproduction or other genetic mechanisms that maintain a high genetic variability in O. neolycopersici. No clear correlation was found between the virulence and the AFLP patterns of the O. neolycopersici isolates studied. The relationship between O. neolycopersici and powdery mildew anamorphs infecting Aquilegia vulgaris, Chelidonium majus, Passiflora caerulea, and Sedum alboroseum was also investigated. These anamorphs are morphologically indistinguishable from and phylogenetically closely related to O. neolycopersici. The crossinoculation tests and the analyses of ITS sequences and AFLP patterns jointly indicated that the powdery mildew anamorphs collected from the above mentioned plant species all represent distinct, but closely related species according to the phylogenetic species recognition. All these species were pathogenic only to their original host plant species, except O. neolycopersici which infected S. alboroseum, tobacco, petunia, and Arabidopsis thaliana, in addition to tomato, in cross-inoculation tests. This is the first genome-wide study that investigates the relationships among powdery mildews that are closely related based on ITS sequences and morphology. The results indicate that morphologically indistinguishable powdery mildews that differed in only one to five single nucleotide positions in their ITS region are to be considered as different taxa with distinct host ranges.

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Infectivity of a Japanese isolate of Oidium neolycopersici KTP-01 to a European tomato cultivar resistant to O. lycopersici

Cited by 36 publications

References 12 publications

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

Consecutive monitoring for conidiogenesis by Oidium neolycopersici on tomato leaves with a high-fidelity digital microscope

Oidium neolycopersici: Intraspecific Variability Inferred from Amplified Fragment Length Polymorphism Analysis and Relationship with Closely Related Powdery Mildew Fungi Infecting Various Plant Species

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