Inflammation, Wound Healing, and Fibrosis

Ghatak, Shibnath; Hascall, Vincent C.; Rodríguez, Ricardo Moreno; Markwald, Roger R.; Misra, Suniti

doi:10.1002/9781119282518.ch15

Cited by 8 publications

(7 citation statements)

References 77 publications

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“…We will report complete set of our findings on functional modulation of the MSCs that experienced the far-from tensional-equilibrium and the Does nomadic cell migration on a heterogeneous mechanical field exist in a living organism? Some types of cells should migrate on micromechanically heterogeneous milieu, such as typically seen in wound healing of disordered tissues 56,57 , in the biological development of immature organs 58,59,60 , and in stem cell localization inside a specific niche 61,62 . In particular, MSCs are delocalized in niches composed of hard bone, soft vessels, and very soft blood cell surroundings in bone marrow, and are nomadically delocalized in the niche with such stiffness-heterogeneity 62 .…”

Section: Discussionmentioning

confidence: 99%

Avoiding tensional equilibrium in cells migrating on a matrix with cell-scale stiffness-heterogeneity

Ebata

Kidoaki

2021

Biomaterials

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Section: Discussionmentioning

confidence: 99%

Avoiding tensional equilibrium in cells migrating on a matrix with cell-scale stiffness-heterogeneity

Ebata

Kidoaki

2021

Biomaterials

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“…12,13 Key aspects of the wound healing process play a critical role in development of fibrosis and particularly in formation of raised dermal scarring. 14,15 Raised dermal scarring often manifests from trauma or an injury to the deep connective tissue layer of the skin and could be considered as a complication or a pathologically abnormal form of the wound healing process. 89 Keloid disease together with other fibrotic conditions including HTS 67 and FKN 10,11 are the end result of uncontrolled inflammation leading to excessive collagen deposition and increased ECM production.…”

Section: Introductionmentioning

confidence: 99%

Immune cells and associated molecular markers in dermal fibrosis with focus on raised cutaneous scars

Kidzeru

Lebeko

Sharma

et al. 2023

Experimental Dermatology

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Raised dermal scars including hypertrophic, and keloid scars as well as scalp‐associated fibrosing Folliculitis Keloidalis Nuchae (FKN) are a group of fibrotic raised dermal lesions that mostly occur following cutaneous injury. They are characterized by increased extracellular matrix (ECM) deposition, primarily excessive collagen type 1 production by hyperproliferative fibroblasts. The extent of ECM deposition is thought to be proportional to the severity of local skin inflammation leading to excessive fibrosis of the dermis. Due to a lack of suitable study models, therapy for raised dermal scars remains ill‐defined. Immune cells and their associated markers have been strongly associated with dermal fibrosis. Therefore, modulation of the immune system and use of anti‐inflammatory cytokines are of potential interest in the management of dermal fibrosis. In this review, we will discuss the importance of immune factors in the pathogenesis of raised dermal scarring. The aim here is to provide an up‐to‐date comprehensive review of the literature, from PubMed, Scopus, and other relevant search engines in order to describe the known immunological factors associated with raised dermal scarring. The importance of immune cells including mast cells, macrophages, lymphocytes, and relevant molecules such as cytokines, chemokines, and growth factors, antibodies, transcription factors, and other immune‐associated molecules as well as tissue lymphoid aggregates identified within raised dermal scars will be presented. A growing body of evidence points to a shift from proinflammatory Th1 response to regulatory/anti‐inflammatory Th2 response being associated with the development of fibrogenesis in raised dermal scarring. In summary, a better understanding of immune cells and associated molecular markers in dermal fibrosis will likely enable future development of potential immune‐modulated therapeutic, diagnostic, and theranostic targets in raised dermal scarring.

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“…Alteplase has been studied extensively and proven useful in the fields of neurology and cardiovascular disease as an emergency intravenous therapy to induce fibrinolysis in cases with occlusion of blood vessels. Prior study of alteplase in ophthalmology revealed it decreased PAI-1 levels significantly in an in vitro experimental study using cultures of HLECs (Song, 2010;Nishi, 2012;Ghatak et al, 2018;Wulandari et al, 2019).…”

Section: Discussionmentioning

confidence: 95%

Attenuation of Transforming Growth Factor-? Expression by Alteplase in Anterior Lens Capsule Fibrosis Model with Fibrin Reaction In Vitro

Sitorus¹,

Mustika²,

Notobroto³

et al. 2021

IJRP

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Background: Underlying pathophysiology of posterior capsule opacification, a frequent visual disturbance postop complication of cataract surgery, is multifactorial. Several cytokines and growth factors, such as Transforming Growth Factor-β (TGF-β), Fibroblast Growth Factor 2 (FGF-2), and Hepatocyte Growth Factor (HGF), have been found to play roles in the formation of PCO. Multiple advancements have been made including researches in therapeutic agents. Alteplase, a fibrinolytic agent, has been studied to decrease fibrin formation, which is a mechanism causing PCO. This study aimed to investigate the effect of alteplase on TGF-β expression in an anterior lens capsule fibrosis model resembling PCO. Methods: An experimental study was performed using cultured anterior lens capsule tissue taken during cataract surgery. Primary cell isolation culture and lens epithelial lens characterization were conducted. Following identification of lens epithelial cell and 90% confluency rate on cell culture, lens epithelial cells were divided into four groups and then scratched to induce inflammatory reaction resembling PCO process after cataract surgery. Doses of 25 mcg/ml, 50 mcg/ml, and 100 mcg/ml were then applied to each group of cell cultures with the other group serving as negative control. Using a fluorescein microscope, measurements to asses the influence of alteplase were taken 7 days after scratching by analyzing the intensity of fluoresce using FITC antibody of TGF-β. Results: The attenuation effect of alteplase to TGF-β expression was observed in all treatment groups; 25 µg/ml (15.5716x106 ±2.56558x10 6 ), 50 µg/ml (12.7364x106 ±1.67067x10 6 ), and 100 µg/ml group (7.8422x106 ±2.24941x106). The Tukey HSD posthoc tests found significant decrease between control group and each treatment group (p=0.019, p=0.000, and p=0.000; respectively). Conclusion: Alteplase within measure dose range holds potential effect in inhibiting fibrosis formation in PCO through attenuation of TGF-β

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Inflammation, Wound Healing, and Fibrosis

Cited by 8 publications

References 77 publications

Avoiding tensional equilibrium in cells migrating on a matrix with cell-scale stiffness-heterogeneity

Avoiding tensional equilibrium in cells migrating on a matrix with cell-scale stiffness-heterogeneity

Immune cells and associated molecular markers in dermal fibrosis with focus on raised cutaneous scars

Attenuation of Transforming Growth Factor-? Expression by Alteplase in Anterior Lens Capsule Fibrosis Model with Fibrin Reaction In Vitro

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