Inflammatory effects of the toxic cyanobacterium Geitlerinema amphibium

Dogo, C. R.; Bruni, Fernanda Miriane; Elias, Fabiana; Rangel, Marisa; Pantoja, Patricia Araujo; Sant’Anna, Célia Leite; Lima, Carla; Lopes-Ferreira, Mônica; Carvalho, Luciana Retz de

doi:10.1016/j.toxicon.2011.07.010

Cited by 22 publications

(10 citation statements)

References 15 publications

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“…and Geitlerinema sp.. Several Pseudanabaena species produce microcystins, with hepatotoxic action (Rangel et al 2014), and are frequently associated with blooms (Kim et al 2006;Willame et al 2006). The genus Geitlerinema, though not yet recorded in blooms, has recently been reported to produce harmful toxins (Dogo et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Potential effects of mechanically removing macrophytes on the phytoplankton community of a subtropical reservoir

Wojciechowski

Burda²,

Scheer³

et al. 2018

Acta Bot. Bras.

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Intensive growth of aquatic macrophytes interferes with water quality and ecosystem dynamics worldwide. Although mechanically removing macrophytes is the most commonly used method for their eradication, it can also cause undesirable disturbances in aquatic reservoir communities. We performed laboratory incubations of phytoplankton sampled before and after macrophytes were mechanically removed from the Piraquara II reservoir, South Brazil. We analyzed changes in growth and composition of the main phytoplankton groups with respect to nutrient shifting. Prior to removing the macrophytes, the phytoplankton community was dominated by low cell abundances of diatoms and flagellates. In contrast, growth rates of cyanobacteria (mainly Cylindrospermopsis raciborskii, Pseudanabaena sp., and Geitlerinema sp.) and of colonial chlorophytes were favored after macrophyte removal, while the abundances of diatoms and flagellates decreased. Our results suggest that removing macrophytes causes dramatic changes in phytoplankton composition and biomass and selects for toxigenic species of cyanobacteria. These changes were probably associated with the disturbance caused by removing the macrophytes, which immediately created new environmental conditions prone to species competition. These findings indicate that the use of mechanical techniques to manage macrophytes should be carefully considered, along with monitoring of harmful species and changes of limnological parameters.

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Section: Discussionmentioning

confidence: 99%

Potential effects of mechanically removing macrophytes on the phytoplankton community of a subtropical reservoir

Wojciechowski

Burda²,

Scheer³

et al. 2018

Acta Bot. Bras.

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“…isolated from Brazil and India have shown the bioactivities on crustaceans ( Artemia salina ), mice, and cancer cell lines (Dogo et al. , Maruthanayagam et al. , Maruthanayagam et al.…”

Section: Discussionmentioning

confidence: 99%

Identification of a new‐to‐science cyanobacterium, Toxifilum mysidocida gen. nov. & sp. nov. (Cyanobacteria, Cyanophyceae)

Zimba

Huang

Foley

et al. 2016

Journal of Phycology

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Cyanobacteria occupy many niches within terrestrial, planktonic, and benthic habitats. The diversity of habitats colonized, similarity of morphology, and phenotypic plasticity all contribute to the difficulty of cyanobacterial identification. An unknown marine filamentous cyanobacterium was isolated from an aquatic animal rearing facility having mysid mortality events. The cyanobacterium originated from Corpus Christi Bay, TX. Filaments are rarely solitary, benthic mat forming, unbranched, and narrowing at the ends. Cells are 2.1 × 3.1 μm (width × length). Thylakoids are peripherally arranged on the outer third of the cell; cyanophycin granules and polyphosphate bodies are present. Molecular phylogenetic analysis in addition to morphology (transmission electron microscopy and scanning electron microscopy) and chemical composition all confirm it as a new genus and species we name Toxifilum mysidocida. At least one identified Leptolyngbya appears (based on genetic evidence and TEM) to belong to this new genus.

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“…The augmented number of cells in the lung tissue must be related to the recruitment of inflammatory cells, such as macrophages. The extracts of another Geitlerinema species also exhibited pro-inflammatory activity [26]. Another interesting observation was the evidence of healing processes in the liver, as suggested by the proximity of the centrilobular veins and disorganization of the hepatic parenchyma.…”

Section: Discussionmentioning

confidence: 99%

“…Two species from the Geitlerinema genus exhibited toxicity to mice (i.p.—intraperitoneal administration) due to the presence of pro-inflammatory and antiacetylcholinesterase (anti-AChE) substances in their extracts: the methanol extract (ME) of Geitlerinema unigranulatum CCIBt 3213 [17,26,27] and the acetic acid extract (AE) of Geitlerinema splendidum CCIBt 3223 [17,28]. Geitlerinema spp.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

et al. 2014

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Cyanobacteria are common members of the freshwater microbiota in lakes and drinking water reservoirs, and are responsible for several cases of human intoxications in Brazil. Pseudanabaena galeata and Geitlerinema splendidum are examples of the toxic species that are very frequently found in reservoirs in Sao Paulo, which is the most densely populated area in Brazil. In the search for toxic strains collected from water reservoirs and maintained in the Cyanobacterial Culture Collection (CCIBt) of the Institute of Botany of Brazil, the acetic acid extracts (AE) of P. galeata CCIBt 3082 and G. splendidum CCIBt 3223 were analyzed by planar chromatography, which indicated the absence of cyanotoxins. Animal tests were then carried out, and both extracts were found to induce toxic effects in mice when administered intraperitoneally. The present study aimed to investigate whether the oral ingestion of the above mentioned cyanobacteria extracts would also induce toxic effects in mice. Necropsy and histopathological studies were conducted using tissue samples from the animals, which were euthanized one week after the administration of the extracts. The AE of P. galeata did not cause death but did induce transient symptoms, including eyebrow ptosis, straub tail, and pain. The euthanized animals presented hemorrhage in the liver, whereas the histological analysis showed disorganization of the hepatic parenchyma, necrosis, hyperemia, and proximity of the centrilobular vein in the liver. In addition, alterations in the convoluted tubules of the kidneys were observed, and the lungs were unaffected. The AE of G. splendidum caused only one death, and induced transient symptoms, such as dyspnea, paralysis, and pain, in the other mice. The necropsy of the euthanized mice showed hemorrhage in the lungs and liver. The lungs presented hemorrhagic focuses, alveolar collapse, and granulomatous foci. The liver presented hemorrhagic and enlarged sinusoids, hyperemia, proximity of the centrilobular vein, and disorganization of the hepatic parenchyma. Some areas also exhibited an inflammatory infiltrate and calcified tissue inside blood vessels. Necrosis and rupture of the convoluted tubule cells were observed in the kidneys. Further analysis of the both extracts indicated the lack of hemolytic activity, and the presence of two unknown anti-AChE substances in the AE of G. splendidum. Thus, P. galeata and G. splendidum are producers of novel toxins that affect mammals when administered orally.

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Inflammatory effects of the toxic cyanobacterium Geitlerinema amphibium

Cited by 22 publications

References 15 publications

Potential effects of mechanically removing macrophytes on the phytoplankton community of a subtropical reservoir

Potential effects of mechanically removing macrophytes on the phytoplankton community of a subtropical reservoir

Identification of a new‐to‐science cyanobacterium, Toxifilum mysidocida gen. nov. & sp. nov. (Cyanobacteria, Cyanophyceae)

Analysis of the Toxicity and Histopathology Induced by the Oral Administration of Pseudanabaena galeata and Geitlerinema splendidum (Cyanobacteria) Extracts to Mice

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