Influence of a Roundup formulation on glyphosate effects on steroidogenesis and proliferation of bovine granulosa cells in vitro

Perego, Maria Chiara; Caloni, F.; Cortinovis, C.; Schütz, Luís F.; Albonico, Marco; Tsuzukibashi, Denise; Spicer, L. J.

doi:10.1016/j.chemosphere.2017.09.007

Cited by 43 publications

(27 citation statements)

References 39 publications

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“…Nevertheless, various glyphosate formulations seem to have adverse effects on hormonal function, and it has been demonstrated that glyphosate-based herbicides, such as Roundup, have an impact on oxygen reactive species, and it also changes the redox system, resulting therefore in apoptosis [ 10 , 11 ]. Last, Roundup has been demonstrated to have deleterious effect on steroidogenesis and proliferation of bovine granulosa cells [ 12 ], indicating indirectly the impact of glyphosate on female gametes [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Glyphosate on Human Sperm Motility and Sperm DNA Fragmentation

Anifandis

Katsanaki

Lagodonti

et al. 2018

IJERPH

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Glyphosate is the active ingredient of Roundup®, which is one of the most popular herbicides worldwide. Although many studies have focused on the reproductive toxicity of glyphosate or glyphosate-based herbicides, the majority of them have concluded that the effect of the specific herbicide is negligible, while only a few studies indicate the male reproductive toxicity of glyphosate alone. The aim of the present study was to investigate the effect of 0.36 mg/L glyphosate on sperm motility and sperm DNA fragmentation (SDF). Thirty healthy men volunteered to undergo semen analysis for the purpose of the study. Sperm motility was calculated according to WHO 2010 guidelines at collection time (zero time) and 1 h post-treatment with glyphosate. Sperm DNA fragmentation was evaluated with Halosperm® G2 kit for both the control and glyphosate-treated sperm samples. Sperm progressive motility of glyphosate-treated samples was significantly reduced after 1 h post-treatment in comparison to the respective controls, in contrast to the SDF of glyphosate-treated samples, which was comparable to the respective controls. Conclusively, under these in vitro conditions, at high concentrations that greatly exceed environmental exposures, glyphosate exerts toxic effects on sperm progressive motility but not on sperm DNA integrity, meaning that the toxic effect is limited only to motility, at least in the first hour.

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Section: Introductionmentioning

confidence: 99%

The Effect of Glyphosate on Human Sperm Motility and Sperm DNA Fragmentation

Anifandis

Katsanaki

Lagodonti

et al. 2018

IJERPH

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“…A limited number of studies have reported disrupted hormone synthesis or signal transduction in various systems and, based on their findings, proposed that glyphosate is an EDC. These include the rat male reproductive organs [10][11][12] and adrenal gland steroidogenesis [13], as well as cell lines such as rodent Leydig cells [14,15], bovine granulosa cells [16,17], and human embryonic, placental and liver cells [18,19]. In many of these studies the tested substance was a commercial formulation of…”

Section: Introductionmentioning

confidence: 99%

Exposure to a glyphosate-based herbicide formulation, but not glyphosate alone, has only minor effects on adult rat testis

Johansson

Schwartz

Nielsen

et al. 2018

Reproductive Toxicology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Exposure to a glyphosate-based herbicide formulation, but not glyphosate alone, has only minor effects on adult rat testis Running Title: Low dose glyphosate exposure has little effect on adult rat testis.

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“…Formulations containing glyphosate IPA salt, Roundup 3 Plus R , Roundup Biovert R , Amega R , Cargly R and Cosmic R , unlike glyphosate alone, were shown to affect cell proliferation in embryonic cells 360 min upon fertilization of the sea urchin Sphaerechinus granularis inducing a delay in entry into the Mphase in the cell cycle (Marc et al, 2002(Marc et al, , 2004. A Roundup R formulation, but again not glyphosate, was shown to affect cell proliferation and steroid production, as dramatically decreased cell numbers, as well as estradiol and progesterone production were recorded in granulosa cells from beef heifer ovaries upon exposure to Roundup R at 0.01-0.30 mg/ml (corresponding to glyphosate concentrations of 0.0018-0.054 mg/ml) (Perego et al, 2017). Roundup R inhibited the survival of human L-02 hepatocytes (IC 50 = 0.15 mg/ml, corresponding to glyphosate concentration of 0.062 mg/ml) by inducing mitochondrial and DNA damage, changes in membrane integrity and permeability, inhibition of the antioxidant system, and thus, apoptosis (Luo et al, 2017).…”

Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%