Influence of acute and fractional X-irradiation on induction of interferon in vivo

Abstract

Acute X-irradiation with 350 R increased the synthesis of interferons induced by poly I : C and endotoxin in individual sera of BALB/c mice. Fractional irradiation with 4.7 or 9.4 I~ daily up to total dose levels of 728, 977 and 1828 R in three different experiments had no influence on serum interferon levels induced by Semliki Forest Virus (SFV), poly I:C or endotoxin. The Newcastle Disease Virus (NDV) induced interferon synthesis decreased in comparison to the non-irradiated control at a total dose of 400 R and attained the control level on further elevation of the total dose. The anti-NDV antibody production fell to one quarter of the control value after exposure to a total dose of 1828 R.

Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity

“…In fact, the cytokine response to radiation exposure has been widely recognized since the first report by Talas et al [32]. A multitude of cytokines respond within a few hours of exposure to radiation, even at a minimally toxic dose [29, 32–37].…”

“…In fact, the cytokine response to radiation exposure has been widely recognized since the first report by Talas et al [32]. A multitude of cytokines respond within a few hours of exposure to radiation, even at a minimally toxic dose [29, 32–37]. Furthermore, cytokine responses induced by ionizing radiation might vary with the dose of radiation and the types of cells/tissues [33, 34].…”

A Pattern of Early Radiation-Induced Inflammatory Cytokine Expression Is Associated with Lung Toxicity in Patients with Non-Small Cell Lung Cancer

“…Cytokine release in response to ionizing radiation is a documented phenomenon and may play a major role in subsequent radiation induced lung toxicity (reviewed in [14] – [18] . A non-specific acute reaction, or “cytokine storm” usually resolves within 24 hours [19] . Fractionated radiation, however, creates a constant complex stress response and a cytokine profile is different to that induced by a single radiation dose [20] .…”

Interferon Synthesis in Mouse Peritoneal Cells Damaged by X-Irradiation

“…Conflicting data, however, were obtained concerning the need of new DNA synthesis for interferon pro duction [11][12][13]. Our earlier observations indicated that the radioresistant cells are re sponsible for interferon production in irradiated animals and cell systems, as fractionated X-or gamma-irradiation (up to total doses of 2,000 R) or acute irradiation by protons of high energy did not inhibit the synthesis of circulat ing interferon induced by NDV, poly I:C or E.coli endotoxin [1,2,14,15], Moreover, in spleen cells of gamma-irradiated mice we have found that N DVinduced interferon is derived from radioresistant macrophages rather than from lymphocytes [3] in contrast to circulating interferon [4,16]. These results were recently confirmed by N a g a n o and M aehara [17].…”

“…I ml) in peritoneal cells in vitro for 4 and 24 h at 37 . After centrifuging, the supernates were dialyzed at pH 2 for 3 days, adjusted to pH 7.0 and then assayed by the cytopathic effect reduction method on monolayer L cell cultures using cncephalomyocarditis virus as a challenge [2], Treatment with antimetabolites. Actinomycin D, puromycin, mitomycin C and hydroxy urea (Nutritional Biochemical Corp., Cleveland, Ohio) were dissolved in PBS, and ad ministered I h before interferon induction (concentrations indicated in 'Results').…”