Influence of adsorption-desorption kinetics on the performance of chromatographic processes using large-pore supports

Rodrigues, Alírio E.; Ramos, Alex Martins; Loureiro, José M.; Dı́az, Mario; Lu, Z. P.

doi:10.1016/0009-2509(92)85118-u

Cited by 35 publications

(13 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2B compares the experimental plate heights obtained for the IgG pulses with calculated plate heights by the modified van Deemter equation for packings showing forced convective solute transport ( Fig. 2A) (16). At linear velocities greater than 3 cm/min, the plate height is independent of linear velocity, as can be seen in Fig.…”

Section: Resultsmentioning

confidence: 66%

The use of confocal laser scanning microscopy to study the transport of biomacromolecules in a macroporous support

Subramanian

Hommerding

2005

Journal of Chromatography B

View full text Add to dashboard Cite

Large-pore materials or supports resembling polymer conduits are used as packing material in chromatographic operations. Our ongoing research has shown that, when modified with peptides or ligands, chitosan beads that are 800 _m in diameter and have 3.5% solids can be used as matrices in bioseparations. The goal of the present study is to evaluate the transport properties of biomolecules in the modified chitosan beaded matrices. Batch uptake experiments with fluorescently tagged pure human IgG, human IgA and human IgM were conducted to visualize the distribution of binding sites hroughout the bead as well as to evaluate restrictions to diffusion, if any, within the support. The chromatographic performance of the macrobeads was first assessed by the classical height equivalent of a theoretical plate HETP analysis. The independence of HETP on linear flow rates studied suggests that a likely mode of solute transport within the macrobeads may be a combination of convection and diffusion-convective components. By using fluorescent-tagged immunoglobulins, the penetration of the adsorbent particle at different times and different levels of saturation was visually observed. The profiles obtained from dynamic experiments were compared to the profiles obtained from finite bath experiments. With an increase in the incubation time, the degree of penetration increased and the bead interior was saturated with FITC immunoglobulins at the end point of the finite bath experiment. In the dynamic uptake experiment, the degree of penetration was found to be a function of the linear velocity and level of breakthrough. The penetration of the bead radius, at times lower than the predicted diffusion time, suggests that the mode of transport in the chitosan beads is governed by a combination of convective and diffusive forces.

show abstract

Section: Resultsmentioning

confidence: 66%

The use of confocal laser scanning microscopy to study the transport of biomacromolecules in a macroporous support

Subramanian

Hommerding

2005

Journal of Chromatography B

View full text Add to dashboard Cite

show abstract

“…The fact that the structure-forming microglobules are in most of the cases nonporous or possess a low degree of meso-and microporosity allows for an enhanced mass transport between the support and the mobile phase. [4][5][6][7][8][9] In combination with high flow velocities feasible with monolithic packings, this results in fast chromatographic separations with high resolution. Furthermore, the simple preparation process is also favorable for miniaturization, e.g., the preparation of capillary columns.…”

Section: Introductionmentioning

confidence: 99%

Surface‐Functionalized, Ring‐Opening Metathesis Polymerization‐Derived Monoliths for Anion‐Exchange Chromatography

Eder

Huber

Buchmeiser

2007

Macromol. Rapid Commun.

View full text Add to dashboard Cite

Ring‐opening metathesis polymerization (ROMP) was used for the synthesis of monolithic capillary columns with inner diameters of 200 µm. The resulting polymeric monoliths were characterized by inverse size‐exclusion chromatography (ISEC). Surface functionalization was carried out in situ using 2‐(N,N‐dimethylaminoethyl)norborn‐5‐ene‐2‐ylcarboxylic amide (1). The resulting functionalized monoliths were successfully used in anion‐exchange chromatography of oligodeoxynucleotides.magnified image

show abstract

“…Our previous studies have established the usefulness of N,N,N ,N -ethylenediaminetetramethylenephosphonic acidmodified zirconia in the separation of immunoglobulins from complex mixtures [7]. Research-based prediction of mass transport, biological activity behavior, kinetic and thermodynamic parameters that impact protein retention and separation are essential for the integration of chromatographicbased unit-operations into the purification scheme [10][11][12][13][14][15][16]. A quantitative or qualitative knowledge of the parameters involved in the transport of biomolecules in a chromatographic system is needed before improvements may be designed.…”

Section: Introductionmentioning

confidence: 99%

Modeling of immunoglobulin uptake by N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid-modified zirconia particles under static and dynamic conditions

Sarkar

Subramanian

2005

Journal of Chromatography B

View full text Add to dashboard Cite

A matrix developed from N,N,N',N'-ethylenediaminetetramethylenephosphonic acid-modified zirconia beads (further referred to as r_PEZ); 25-38 microm in diameter and with a pore size of 22+/-3 nm, was utilized for the separation of immunoglobulins (Igs). r_PEZ has been shown to bind to various Igs originating from a wide variety of species. To understand the mechanisms controlling the uptake of Igs by r_PEZ, static protein uptake experiments were carried out. The protein uptake profiles were further modeled with a kinetic rate constant model. Individual studies were undertaken for human immunoglobulin A, G and M (HIgA, HIgG and HIgM). The kinetic rate constant model indicated that HIgG binding to r_PEZ was more favorable than its disassociation. The equilibrium rate constants were found to decrease with increasing concentration. The effect of continuous loading in a packed bed system utilizing r_PEZ matrix was evaluated by carrying out frontal studies, using different feed concentrations and linear velocities. The breakthrough profiles obtained for the uptake of HIgG were modeled with the pore diffusion model. The model was found to best describe the breakthrough profiles obtained at a feed concentration of 2.0 mg of HIgG per milliliter. The NTU for the packed bed was found to be equal to 2.

show abstract

Influence of adsorption-desorption kinetics on the performance of chromatographic processes using large-pore supports

Cited by 35 publications

References 9 publications

The use of confocal laser scanning microscopy to study the transport of biomacromolecules in a macroporous support

The use of confocal laser scanning microscopy to study the transport of biomacromolecules in a macroporous support

Surface‐Functionalized, Ring‐Opening Metathesis Polymerization‐Derived Monoliths for Anion‐Exchange Chromatography

Modeling of immunoglobulin uptake by N,N,N′,N′-ethylenediaminetetramethylenephosphonic acid-modified zirconia particles under static and dynamic conditions

Contact Info

Product

Resources

About