Influence of age on aluminum induced lipid peroxidation and neurolipofuscin in frontal cortex of rat brain: A behavioral, biochemical and ultrastructural study

Tripathi, Sandeep; Mahdi, Abbas Ali; Nawab, Akbar; Chander, Ramesh; Hasan, Mahdi; Siddiqui, M. Shakil; Mahdi, Farzana; Mitra, Kalyan; Bajpai, V.K.

doi:10.1016/j.brainres.2008.11.060

Cited by 57 publications

(45 citation statements)

References 42 publications

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“…Also, Al could increase the activity of monoamine oxidase enzyme (MAO) in the brain which leads to increase generation of H 2 O 2 [26]. Aluminum can induce lipid peroxidation and alter physiological as well as biochemical behavior of living organism, a matter implicated in increased brain MDA level which is the major aldehyde formed after breakdown of lipid hydroperoxides [27].…”

Section: Discussionmentioning

confidence: 99%

Does Melatonin Ameliorate Neurological Changes Associated With Alzheimer’s Disease in Ovariectomized Rat Model?

et al. 2012

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This study aimed to elucidate the mechanisms of melatonin to manage neurological damage in Alzheimer's disease (AD) induced in ovariectomized rats. Forty adult female rats were enrolled in our study and were classified as; gonad intact control, ovariectomized control group, ovariectomized rats received melatonin, ovariectomized rats injected with AlCl 3 to induce AD and ADinduced rats treated with melatonin. Hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), B cell lymphoma 2 (Bcl-2), brain derived neurotrophic factor (BDNF), acetylcholinesterase (AchE) and acetylcholine (Ach) were estimated in the brain tissues of the different groups. Treatment of AD-induced rats with melatonin produced marked improvement in the most studied biomarkers which was confirmed by histological investigation of the brain. In Conclusion, melatonin significantly ameliorates the neurodegeneration characteristic of AD in experimental animal model due to its antioxidant, antiapoptotic, neurotrophic and anti-amyloidogenic activities.

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Section: Discussionmentioning

confidence: 99%

Does Melatonin Ameliorate Neurological Changes Associated With Alzheimer’s Disease in Ovariectomized Rat Model?

et al. 2012

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“…Reactive free radicals, including both reactive oxygen species (ROS) and reactive nitrogen species (RNS), could be substantially produced in response to multiple stresses, in different cellular sub compartments [30][31][32]. ER provides an exclusive oxidizing environment to the proteins to facilitate disulfide bond formation [33][34][35], and the ROS generated as a result of this process in ER alone contributes to 25 % of ROS generated by the cell [36].…”

Section: Er Stress and Oxidative Stressmentioning

confidence: 99%

Role of Endoplasmic Reticulum Stress and Unfolded Protein Responses in Health and Diseases

2015

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Endoplasmic reticulum (ER) is the site of protein synthesis, protein folding, maintainance of calcium homeostasis, synthesis of lipids and sterols. Genetic or environmental insults can alter its function generating ER stress. ER senses stress mainly by three stress sensor pathways, namely protein kinase R-like endoplasmic reticulum kinase-eukaryotic translation-initiation factor 2a, inositol-requiring enzyme 1a-X-box-binding protein 1 and activating transcription factor 6-CREBH, which induce unfolded protein responses (UPR) after the recognition of stress. Recent studies have demonstrated that ER stress and UPR signaling are involved in cancer, metabolic disorders, inflammatory diseases, osteoporosis and neurodegenerative diseases. However, the precise knowledge regarding involvement of ER stress in different disease processes is still debatable. Here we discuss the possible role of ER stress in various disorders on the basis of existing literature. An attempt has also been made to highlight the present knowledge of this field which may help to elucidate and conjure basic mechanisms and novel insights into disease processes which could assist in devising better future diagnostic and therapeutic strategies.

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“…GSH/GSSG ratio determines the relative amount of reduced or working glutathione (GSH) compared to the oxidized glutathione (GSSG). A larger ratio reflects a more efficient glutathione redox system, because GSH is normally maintained in a highly reduced state of via NADPH dependent enzymes, specifically GR, while, GPx has protects from the H 2 O 2 which produced during the metabolism of superoxide radicals [7].…”

Section: Resultsmentioning

confidence: 99%

“…Increased lipid peroxidation (LPO) is one of the major consequences of oxidative stress [6]. Lipofuscin, a morphological biomarker of lipid peroxidation, represents an end -product of oxidative degradation of lipids by free radical mechanism [7]. Studies on age associated and fluoride mediated toxic effects are very important as there are several reports that, on one hand the efficiency of the blood brain barrier (BBB) may decline with advancing age [8], and on the other hand, cognitive decline may account for functional and morphological changes in the central nervous system (CNS).…”

Section: Introductionmentioning

confidence: 99%

Age Associated Fluoride Induced Ultra Structural and Biochemical Changes in Rat Brain

Tripathi¹

2015

MOJT

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Materials and Methods AnimalsForty male Rattus norigious, Wistar strain rats of two age groups, young (6 month, weight 182 ± 2.2g, n = 24) and old (24 AbstractFluoride (F) ingestion is well known to be associated with oxidative stress in experimental animals, but the precise mechanism of its toxicity associated with advancing age has not yet been delineate. The present study attempts to assess the learning and memory capacity of rats using water maze test for cognitive functioning. The markers of oxidative stress i.e., Reacting oxygen species (ROS), protein carbonyl content (PC), lipid peroxide levels (LPx), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were measured in the brain hippocampus of young and aged rats fed with fluoride (100 ppm) with drinking for 90 days. We observed significant changes of fluoride treated young and aged rat as compared with their respective controls. Lipofuscin content was significantly increased in fluoride treated aged rats. Ultra structural studies of the CA1 region in hippocampus of fluoride exposed rats revealed that the changes were more pronounced in the aged treated rats in terms of presence of clustered lipofuscin, vacuolization, lysosomal degradation and nucleolar fragmentation. On the basis of the results it may conclude that fluoride may be linked with neuro lipofuscinogenesis and alteration in neurobehavioral activity and these changes may be responsible for the development of age related disorders. month, weight 466 ± 2.5g, n = 24), were taken from animal house of the NIMS University, Jaipur, India. The animals were separately housed in polypropylene cages in a room, which was maintained at a temperature of 22 ± 2°C, relative humidity of 50 ±10% and 12h light dark cycles. They were fed a commercial pellet diet and allowed access to water ad libitum. The Institutional Animal Ethics Committee approved the study prior to the initiation of the experiment and also approved all experimental protocols. Keywords TreatmentGroups of young (n = 20) and aged (n = 20) animals were randomly divided into two subgroups (n = 10) viz. young control (YC), Young Experimental (YEx) and Old control (OC) and old experimental (OEx). 100 ppm fluoride was given through drinking water to experimental groups of young and old rats for 90 days. Tissue Homogenate PreparationAfter 90 days of Al administration rats were sacrificed their brain were removed and weighed individually. Thereafter, hippocampus was dissected out for biochemical analysis. Ten percent (w/v) homogenate of the frontal cortex was prepared by of York's homogenizer fitted with Teflon plunger in 0.1 M phosphate buffer (pH 7.1). The whole homogenate was first centrifuged at 2500 x g for 10 minutes in a refrigerated centrifuge. The pellet consisting of nuclear fraction and cell debris was discarded. The supernatant was further centrifuged at 11,000 x g for 15 minutes and mitochondrial fraction was separated. The clear supernatant was further centrifuged at 105,000 x g for 90 m...

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Influence of age on aluminum induced lipid peroxidation and neurolipofuscin in frontal cortex of rat brain: A behavioral, biochemical and ultrastructural study

Cited by 57 publications

References 42 publications

Does Melatonin Ameliorate Neurological Changes Associated With Alzheimer’s Disease in Ovariectomized Rat Model?

Does Melatonin Ameliorate Neurological Changes Associated With Alzheimer’s Disease in Ovariectomized Rat Model?

Role of Endoplasmic Reticulum Stress and Unfolded Protein Responses in Health and Diseases

Age Associated Fluoride Induced Ultra Structural and Biochemical Changes in Rat Brain

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