Influence of Agrobacterium rhizogenes Strains on Biomass and Alkaloid Productivity in Hairy Root Lines of Hyoscyamus muticus and H. albus *

Zehra, M.; Banerjee, Santanu; Sharma, Shruti; Kumar, Sushil

doi:10.1055/s-1999-13964

Cited by 33 publications

(17 citation statements)

References 10 publications

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“…This is consistent with data on hairy roots of Hyoscyamus albus, where the relationship between the bacterial strain and alkaloid production was also observed (Zehra et al, 1999). We found the hairy roots differed in their content of RA even when the same Agrobacterium strain A4 was used, suggesting that it might be a consequence of the different sites of T-DNA insertion into the plant genome (Zehra et al, 1999). We also demonstrated that RA levels were always slightly higher in sage root cultures grown in the light than in the dark, but the differences were statistically significant only for line HR-1 (Fig.…”

Section: Resultssupporting

confidence: 92%

“…It is also possible that the differences in RA production between hairy root lines can be originated from dissimilarities of the bacteria used for infection because RA accumulation was significantly higher in root cultures induced by strain ATCC 15834 than in those transformed with A4. This is consistent with data on hairy roots of Hyoscyamus albus, where the relationship between the bacterial strain and alkaloid production was also observed (Zehra et al, 1999). We found the hairy roots differed in their content of RA even when the same Agrobacterium strain A4 was used, suggesting that it might be a consequence of the different sites of T-DNA insertion into the plant genome (Zehra et al, 1999).…”

Section: Resultssupporting

confidence: 91%

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Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

Grzegorczyk

Królicka

Wysokińska

2006

Zeitschrift Für Naturforschung C

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Shoots of Salvia officinalis, a medicinally important plant, were infected with Agrobacterium rhizogenes strains ATCC 15834 and A4 which led to the induction of hairy roots in 57% and 37% of the explants, respectively. Seven lines of hairy roots were established in WP liquid medium under light and dark conditions. The transformed nature of the root lines was confirmed by polymerase chain reaction using rolB and rolC specific primers. Transformed root cultures of Salvia officinalis showed variations in biomass and rosmarinic acid production depending on the bacterial strain used for transformation and the root line analyzed. Both parameters (growth and rosmarinic acid content) of ATCC 15834-induced lines were significantly higher than the A4-induced lines. The maximum accumulation of rosmarinic acid (about 45 mg g Ð1 of dry weight) was achieved by hairy root line 1 (HR-1) at the end of the culture period (45Ð50 days). The level was significantly higher than that found in untransformed root culture (19 mg g Ð1 of dry wt).

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 91%

Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

Grzegorczyk

Królicka

Wysokińska

2006

Zeitschrift Für Naturforschung C

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“…However, higher virulence of the SA79 strain over other A. rhizogenes strains including the A4 could also be noted in case of Bacopa monnieri (Bansal et al 2014) contradicting our present observation. Host plant-specific receptiveness towards designated A. rhizogenes strain, in combination with the compatibility threshold of certain explants, might primarily be accountable for the presently observed trend, which has already been exemplified in several earlier studies (Zehra et al 1999;Akramian et al 2008;Roychowdhury et al 2013).…”

Section: Induction and Establishment Of Hairy Rootssupporting

confidence: 56%

Advances in Boerhaavia diffusa hairy root technology: a valuable pursuit for identifying strain sensitivity and up-scaling factors to refine metabolite yield and bioactivity potentials

et al. 2015

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The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammatory) potentials. Host plant-specific receptiveness towards A. rhizogenes strains and disparity in compatibility threshold of leaf and nodal explants were evident. Only leaf explants responded, attaining hairy root induction with the ATCC 15834 followed by A4 and SA79 strains in reducing order of transformation efficiency. The growth behaviours differed amongst independent rhizoclones, and two clones of A4 (RBH) and ATCC 15834 (RBT8) origin demonstrated higher growth potentials. Polymerase chain reaction amplification of rol genes confirmed their transformed nature. Optimization of the appropriate solvent and reverse phase high-performance liquid chromatography parameters relating to the targeted metabolite production in the selected RBH and RBT8 clones revealed higher accumulation of eupalitin with the RBH clone having the best result of 1.44 times greater yield over the control root. Compared to the selected rhizoclones, the control roots however showed higher boeravinone B content. Devising a modified "stirred-tank" reactor through equipping with marine impellers and ring spargers facilitated high-density RBH root biomass yield with 6.1-fold and 1.15-fold yield increment of the boeravinone B and eupalitin respectively compared to shake-flask cultures. Considering the control roots, the RBH clone revealed analogous antioxidant/antibacterial activities with improved anti-inflammatory potential. The hairy root mediated higher production of boeravinone B and eupalitin could be achieved for the first time in bioreactor.

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“…No adventitious root was formed from control explants. Strain ATCC11325 failed to induce roots from any of the (Zehra et al 1999). Strains R1000 and A 4 GUS behave differently in present study because they have different chromosomal virulence genes (see Materials and methods).…”

Section: Resultsmentioning

confidence: 65%

Agrobacterium rhizogenes mediated transformation of Scutellaria baicalensis and production of flavonoids in hairy roots

Tiwari¹,

Trivedi²,

Guang³

et al. 2008

Biologia plant.

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Using different explants of in vitro seed grown Scutellaria baicalensis Georgi plantlets, hairy roots were induced following inoculation of Agrobacterium rhizogenes strains A 4 GUS, R1000 LBA 9402 and ATCC11325. The A 4 GUS proved to be more competent than other strains and the highest transformation rates were observed in cotyledonary leaf explant (42.6 %). The transformed roots appeared after 15 -20 d of incubation on hormone free Murashige and Skoog medium. Growth of hairy roots was assessed on the basis of total root elongation, lateral root density and biomass accumulation. Maximum growth rate was recorded in root:medium ratio 1:100 (m/v). Hairy root lines were further established in Gamborg B 5 medium and the biomass increase was maximum from 15 to 30 d. PCR, Southern hybridization and RT-PCR confirmed integration and expression of left and right termini-linked Ri T-DNA fragment of the Ri plasmid from A 4 GUS into the genome of Scutellaria baicalensis hairy roots. GUS assay was also performed for further integration and expression. All the clones showed higher growth rate them non-transformed root and accumulated considerable amounts of the root-specific flavonoids. Baicalin content was 14.1 -30.0 % of dry root mass which was significantly higher then that of control field grown roots (18 %). The wogonin content varies from 0.08 to 0.18 % among the hairy root clones which was also higher than in non-transformed roots (0.07 %).

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Influence of Agrobacterium rhizogenes Strains on Biomass and Alkaloid Productivity in Hairy Root Lines of Hyoscyamus muticus and H. albus *

Cited by 33 publications

References 10 publications

Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

Advances in Boerhaavia diffusa hairy root technology: a valuable pursuit for identifying strain sensitivity and up-scaling factors to refine metabolite yield and bioactivity potentials

Agrobacterium rhizogenes mediated transformation of Scutellaria baicalensis and production of flavonoids in hairy roots

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