Influence of bispyribac sodium on nitrogenase activity and growth of cyanobacteria isolated from paddy fields

Tolerance limit, kinetics of nitrogen uptake and activity of nitrate reductase (NR), nitrite reductase (NiR) and glutamine synthetase (GS) enzymes of cyanobacterium Desmonostoc muscorum PUPCCC 405.10 were studied under the regime of chloroacetanilide herbicide pretilachlor. The organism was isolated from the paddy field under application of the herbicide and tolerated pretilachlor up to 10 ppm under laboratory conditions. The incubation of the cyanobacterium in 2.5 ppm pretilachlor did not cause significant effect on N uptake while supplementation of 5-10 ppm pretilachlor in medium reduced the rate of nitrate and nitrite uptake by 35-73 % with 50 % decrease in V max and no change in K m . These results indicated that herbicide did not affect the affinity of uptake system to nitrate and nitrite. In presence of herbicide, the activity of nitrogen assimilation enzymes was inhibited by 16 % for NR and 18 % for NiR. Unchanged K m and decreased V max (50-60 %) in presence of herbicide indicated non-competitive-type inhibition of the enzymes. A 50 % decrease in V max and K m of ammonium uptake indicated un-competitive type inhibition of the ammonium transport system by the herbicide. GS activity also exhibited non-competitive inhibition in presence of pretilachlor with 21 % decrease in activity with unchanged K m and 40 % decrease in V max . Likewise, decreases in nitrogenase activity by 43 % and heterocyst formation by 40 % were recorded in presence of herbicide. Results indicated that pretilachlor affected nitrogen assimilation at uptake level and interacted with nitrogen-assimilating enzymes in a non-competitive manner.

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Agronomic and environmental aspects of diazotrophic bacteria in rice fields

Pittol

Durso

Valiati

et al. 2015

Ann Microbiol

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Influence of bispyribac sodium on nitrogenase activity and growth of cyanobacteria isolated from paddy fields

Abstract: The goal of this study was to determine the effect of bispyribac-sodium on the nitrogenase activities and growth of cyanobacteria isolated from paddy fields. Ten cyanobacterial species were used in this study.

Cited by 9 publications

References 42 publications

Herbicide risks to non-target species and the environment: A review

Herbicide risks to non-target species and the environment: A review

Effect of pretilachlor on nitrogen uptake and assimilation by the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

Agronomic and environmental aspects of diazotrophic bacteria in rice fields

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