Influence of canine donor plasma hemostatic protein concentration on quality of cryoprecipitate

Abstract

BackgroundCryoprecipitate (CRYO) is a plasma component containing high concentrations of factor VIII (FVIII), von Willebrand factor (VWF), and fibrinogen. Because Greyhounds are reported to have lower plasma VWF and fibrinogen concentrations, their plasma may not yield high potency CRYO.ObjectivesTo determine if plasma hemostatic protein concentration is a good predictor of CRYO potency and if a difference exists in quality of CRYO prepared from Greyhounds versus non‐Greyhounds.AnimalsTwenty Greyhounds and 20 non‐Greyhounds.MethodsA 450 mL unit of blood was collected from each donor, centrifuged to prepare fresh frozen plasma (FFP), and processed to CRYO. Aliquots of FFP and CRYO were analyzed for FVIII, VWF, and fibrinogen content and factor recovery.ResultsA positive correlation was found among donor plasma FVIII, VWF and fibrinogen concentration, and CRYO factor content (P < .001). Mean recovery was highest for VWF (67%), followed by fibrinogen (47%), and FVIII (37%). No breed difference was found in mean CRYO FVIII content, but CRYO VWF and fibrinogen were lower in Greyhounds (P = .004 and P < .001, respectively). No difference was found between Greyhounds and non‐Greyhounds for the number of CRYO units meeting human blood banking standards.Conclusions and Clinical ImportanceFactor concentration in FFP is associated with CRYO potency, suggesting that prescreening of blood donors may enhance CRYO quality. Despite lower VWF and fibrinogen content, CRYO prepared from Greyhounds is acceptable based on blood banking standards for humans, indicating that Greyhound FFP does not need to be excluded from CRYO production.

Stability of ex vivo coagulation factor activity in never‐frozen and thawed refrigerated canine plasma stored for 42 days

“…greyhounds have been shown in previous studies to have significantly lower activity of FX, vWF, and fibrinogen concentrations when compared to non-greyhound blood donors. 13,24 Nonetheless, most blood banks have a combination of greyhound and non-greyhound donors, thus our findings are likely generalizable. Blood banks that do not use greyhound donors could consider reinterpreting our data with the exclusion of the greyhounds.…”