Stability of ex vivo coagulation factor activity in never‐frozen and thawed refrigerated canine plasma stored for 42 days

Chee, Weiqin; Sharp, Claire R.; Boyd, Corrin J.; Claus, Melissa A.; Smart, Lisa

doi:10.1111/vec.13152

Cited by 5 publications

(18 citation statements)

References 26 publications

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“…However, given the stability of all other factors, it appears suitable for most other indications for hemostatic transfusion in dogs. Interestingly, and again consistent with previous human studies (66, 67), vWF:Ag in our study reached 50% activity 11 days earlier than that from a study of platelet-poor canine plasma (64).…”

Section: Discussionsupporting

confidence: 93%

“…Only FVIII activity fell below 50% before d14 of storage. While canine and human FVIII activity in cold-stored plasma typically declines earlier than other coagulation factors (60)(61)(62)(63), it declined much earlier in our study compared to an investigation of never-frozen liquid platelet-poor canine plasma, where 50% activity was not reached until d35 of storage (64) and was not reached at all by the end of the storage period (14 days) in another study (65). However, our data is consistent with declines in FVIII activity in human CSWB (21,22,25).…”

Section: Discussioncontrasting

confidence: 51%

“…Finally, the ACL TOP R has only undergone analytical validation for PT, aPTT, fibrinogen (Clauss method) and D-dimers in dogs (37). Coagulation factor activity data for canine FV, FVII, FVIII, FIX, FX, vWF:Ag has been previously reported for this device, but has not undergone analytical validation (64,73). Additionally, the activity of canine FII and FXIII:Ag measured using the ACL TOP R have not previously been reported in dogs.…”

Section: Discussionmentioning

confidence: 99%

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The hemostatic profile of cold-stored whole blood from non-greyhound and greyhound dogs over 42 days

Cooper

Sharp²,

Boyd³

et al. 2023

Front. Vet. Sci.

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ObjectivesTo compare the hemostatic characteristics of cold-stored whole blood (CSWB) from non-greyhound dogs (NGD) and greyhound dogs (GD) over 42 days of storage, notably, platelet closure time (PCT) (NGD only), manual platelet count (PLT) (GD only), ellagic acid (INTEM) and tissue factor activated (EXTEM) rotational thromboelastometry, prothrombin (PT) and activated partial thromboplastin time (aPTT), fibrinogen concentration (FIB), and the activities of factors (F) FII, FV, FVII, FVIII, FIX, FX, FXIII antigen (FXIII:Ag), and von Willebrand factor antigen (vWF:Ag).DesignWhole blood from 10 NGD and 10 GD, was refrigerated in CPD blood bags at 4°C for 42 days. Blood was analyzed before refrigeration (day 0) and at day 1 (d1), 3, 5, 7, 10, 14, 17, 21, 24, 28, 31, 35, 38, and 42. Multivariate linear mixed effects models were created to evaluate coagulation parameters over time and compare NGD and GD. Data are summarized as estimated marginal means with 95% confidence intervals. Significance was set at P < 0.05.ResultsThe PCT for all NGD CSWB was above the device limit by d7. The PLT for GD CSWB did not change during storage. The mean alpha-angle for INTEM and EXTEM decreased to <50% of baseline at d38 and d31 for NGD, and d31 and d17 for GD CSWB. The mean maximum clot firmness (MCF) for INTEM and EXTEM reduced to <50% of baseline at d42 and d28 for both GD and NGD. PT and aPTT for NGD and GD increased over time. For NGD CSWB, the mean FVIII and vWF:Ag activities decreased to <50% of baseline at d7 and d28, respectively, and FIB reached 0.982 g/dL by d24. For GD CSWB, FVIII, FXIII:Ag and FV activities decreased to <50% of baseline by d3, d38, and d38, respectively, and FIB was 0.982 g/dL at baseline. Alpha-angle and MCF for both INTEM and EXTEM, and activities for FII, FV, FIX, FXIII:Ag were significantly lower, and vWF:Ag was significantly higher overall in GD CSWB compared with NGD. A significant difference in the pattern of change over time was detected between NGD and GD in EXTEM alpha-angle, INTEM and EXTEM MCF, FII, and FVIII activities.ConclusionsThe in vitro viscoelastic parameters of GD and NGD CSWB declines over 42 days, but numerous hemostatic parameters (INTEM and EXTEM alpha-angle and MCF, activity of FII, FV, FV, FVII, FIX, FX, FXIII:Ag, vWF:Ag, and FIB) remain within 50% of baseline for more than 14 days. CSWB from GD compared to NGD has reduced hemostatic activity overall, but a similar pattern of decline for most parameters over time.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 51%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The hemostatic profile of cold-stored whole blood from non-greyhound and greyhound dogs over 42 days

Cooper

Sharp²,

Boyd³

et al. 2023

Front. Vet. Sci.

Self Cite

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“…16 While the risk of bacterial contamination in canine FFP kept at room temperature has not been previously evaluated in dogs, in other previous studies, bacterial growth did not occur in never-frozen refrigerated or thawed refrigerated canine plasma despite storage for up to 42 days. 17,18 Another study revealed that canine-specific albumin, when refrigerated at 4 • C, can be stored for up to 24 hours after reconstitution without evidence of bacterial growth. 19 Another concern of keeping FFP units at room temperature for 12 hours was the stability of labile plasma factors, such us factors V and VIII.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of bacterial growth, effects on albumin, and coagulation factors in canine fresh frozen plasma administered as continuous rate infusion exposed to room temperature for 12 hours

Mesa-Sanchez

Ferreira

Blasi-Brugué

et al. 2023

J Vet Emergen Crit Care

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ObjectivesTo determine the risk of bacterial growth and to analyze the stability of albumin and coagulation factors in canine fresh frozen plasma (FFP) units exposed to room temperature (24°C) administered as a continuous rate infusion (CRI) for 12 hours.DesignEx vivo study.SettingUniversity teaching hospital and pet blood bank.AnimalsNone.InterventionsNone.Measurements and Main ResultsAn FFP CRI was simulated to replicate the standard routine procedure used in dogs. Plasma samples were collected before starting the CRI (H0), after 4 hours (H4), and after 12 hours (H12). Bacterial culture of FFP was performed and albumin concentration and specific activity levels for factors V, VII, VIII, and IX were measured and compared. All plasma culture results were negative. There were no statistically significant differences at any time point in the factor VIII activity (median 105.5% [range, 75.6%–142.0%] at H0; median 107.8% [range, 75.0%–172.7%] at H4; and median 112.1% [range, 81.7%–171.0%] at H12); factor IX activity (median 119.3% [range, 89.1%–175.9%] at H0; median 123.1% [range, 72.5%–172.7%] at H4; and median 118.3% [range, 86.6%–177.5%] at H12); or albumin concentration (median 21.0 g/L [range, 17.0–23.0 g/L] at H0 and median 20.0 g/L [range, 17.0–24.0 g/L] at H12). A slight but significant increase in factor V activity was observed when comparing H0 (median 107.0% [range, 71.0%–159.0%]) to H4 (median 117.7% [range, 71.0%–176.7%]) (P = 0.002) or H12 (median 116.2% [range, 71.0%–191.6%]) (P = 0.001). A slight but significant increase in factor VII activity was observed when comparing H0 (median 115.4% [range, 70.6%–183.7%]) to H4 (median 118.2% [range, 82.7%–194.6%]) (P = 0.005); H0 to H12 (median 128.7% [range, 86.4%–200.0%]) (P < 0.001); and H4 to H12 (P = 0.002).ConclusionsFFP CRI at room temperature for 12 hours could be considered safe with regard to risk for bacterial growth and also effective by providing albumin and clotting factors.

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“…Fibrinogen concentration was measured using the manufacturer’s reagent for the Clauss method, calibrated using the manufacturer’s calibration plasma. All remaining coagulation factor activities were calibrated with canine pooled plasma, as previously described [ 20 ]. Activity of FV, FVII, and FX were measured using a modified PT test, while FVIII and FIX were measured using a modified APTT test, all using the manufacturer’s specific factor-depleted plasmas.…”

Section: Methodsmentioning

confidence: 99%

Biomarkers of Coagulation and Inflammation in Dogs after Randomized Administration of 6% Hydroxyethyl Starch 130/0.4 or Hartmann’s Solution

Boyd

Raisis

Sharp

et al. 2022

Animals

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Synthetic colloid fluids containing hydroxyethyl starch (HES) have been associated with impairment of coagulation in dogs. It is unknown if HES causes coagulation impairment in dogs with naturally occurring critical illness. This study used banked plasma samples from a blinded, randomized clinical trial comparing HES and balanced isotonic crystalloid for bolus fluid therapy in 39 critically ill dogs. Blood was collected prior to fluid administration and 6, 12, and 24 h thereafter. Coagulation biomarkers measured at each time point included prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen concentration, and the activities of coagulation factors V, VII, VIII, IX, and X, von Willebrand factor antigen, antithrombin, and protein C. Given the links between coagulation and inflammation, cytokine concentrations were also measured, including interleukins 6, 8, 10, and 18, keratinocyte-derived chemokine, and monocyte chemoattractant protein-1. Data were analyzed with linear mixed effects models. No significant treatment-by-time interactions were found for any biomarker, indicating that the pattern of change over time was not modified by treatment. Examining the main effect of time showed significant changes in several coagulation biomarkers and keratinocyte-derived chemokines. This study could not detect evidence of coagulation impairment with HES.

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Stability of ex vivo coagulation factor activity in never‐frozen and thawed refrigerated canine plasma stored for 42 days

Cited by 5 publications

References 26 publications

The hemostatic profile of cold-stored whole blood from non-greyhound and greyhound dogs over 42 days

The hemostatic profile of cold-stored whole blood from non-greyhound and greyhound dogs over 42 days

Evaluation of bacterial growth, effects on albumin, and coagulation factors in canine fresh frozen plasma administered as continuous rate infusion exposed to room temperature for 12 hours

Biomarkers of Coagulation and Inflammation in Dogs after Randomized Administration of 6% Hydroxyethyl Starch 130/0.4 or Hartmann’s Solution

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