High-tunnel strawberry (Fragaria ×ananassa) production for extended seasons has a great economic potential for small farmers. However, information on cultivars that are suitable for high tunnels is rather limited. In this study conducted in the 2014–15 season, strawberry plugs of eight June-bearing cultivars (Florida Radiance, Benicia, Camarosa, Camino Real, Chandler, Strawberry Festival, Sweet Charlie, and Winterstar) and two day-neutral cultivars (San Andreas and Albion) were evaluated for yield performance, fruit quality, and vegetative growth in organically managed high tunnels at two locations in North Carolina. Significant cultivar differences in whole-season yield were observed at Greensboro, NC; but not at Goldsboro, NC. The cultivar Florida Radiance had the highest marketable and total yields, followed by Winterstar and Chandler at Greensboro, whereas Benicia, Winterstar, and Chandler were the top producing cultivars at Goldsboro. Harvest of day-neutral cultivars San Andreas and Albion started in November. For June-bearing cultivars, Florida Radiance began to produce harvestable berries in late December, followed by Winterstar in early January. Peak harvest occurred in April for all cultivars. At the end of the season, ‘Albion’ had smaller canopy size than other cultivars. It also developed the fewest number of branch crowns and least aboveground biomass. Total soluble solid (TSS) content in April was lower than that observed early and late in the season for all cultivars, although Strawberry Festival exhibited a relatively stable TSS throughout the season. ‘Benicia’ produced the largest strawberries in the early season, but its fruit weight was remarkably reduced as the season progressed. Severe frost events occurred on 18 and 20 Feb. that caused an average of 61.5% and 32.2% open blossom damage at Greensboro and Goldsboro, respectively. The recommended cultivars based on this 1-year study are Florida Radiance, Benicia, and Camino Real for June-bearing cultivars, and Albion and San Andreas for day-neutral cultivars.