Influence of D-asparagine on the engraftment of a biopolymer scaffold for replacing skin defects

Abstract

Оригинальные исследОванияЭкспериментальная медицина E arly necrectomy with skin autograft primary autoplasty is the method of choice in the treatment of burn injury patients [1, 2]. However, questions remain regarding the quality of skin healing, the formation of dermal scars, and the lack of donor material in patients with extensive grade III burns [1,3]. In this regard, the development of biodegradable hydrogel scaffolds (based on biopolymers of natural origin, such as sodium alginate and gelatin) capable of performing the role of wound coating and regenerative matrix is an actual and promising approach in solving of these problems [4, 5].Under experimental conditions, there is significant potential for influencing the dynamics of cellular reactions, the nature of colonization, and remodeling of the scaffold after its implantation by using modulators of the proliferative activity of dermal matrix cells, including those based on a natural metabolite such as D-asparagine [6-8], which expands the possibilities for studying it in burn injury models.The aim of the study was to determine the effect of D-asparagine on the engraftment of a biopolymer scaffold in the replacement of skin defects after burn wounds necrectomy in rats.Material and Methods. Twenty inbred male Wistar rats weighing 270-300 g were used in this study. All interventions capable of causing pain or suffering to animals were performed under anesthesia using 20 mg/ kg tiletamine hydrochloride (Zoetis Inc., USA) and 6 mg/ kg xylazine hydrochloride (NITA-PHARM, Russia), both applied intramuscularly. When performing traumatic manipulations with an antimicrobial purpose, animals were injected with 2.5 mg/kg gentamicin sulfate (Agrofarm, Russia) intramuscularly.