Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid

Taso, Ervin; Stefanovic, Vladimir; Stevanović, Ivana; Vojvodić, Danilo; Topić, Aleksandra; Petković-Ćurčin, Aleksandra; Obradović-Djuričić, Kosovka; Marković, A; Djukić, Mirjana; Vujanović, Dragana

doi:10.1155/2018/1823189

Cited by 10 publications

(8 citation statements)

References 35 publications

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“…On the other hand, locally produced IL-1β and TNF-α exert significant influence on odontoblasts functions, inducing further β-defensin production 22 , production of dental matrix protein -1 and inducing proliferation of odontoblast-like cells derived from stem cells. 23 Moreover, the studies investigated the cytokine profile in the GCF samples following dental restoration and reported controversial data 24,25,26,27,28,29,30 The hypothesis of the present study was that IL-1β and TNF-α profile in the GCF from caries affected and intact teeth are different, while the caries extension, tooth position, and different restorative biomaterials alters the biomarker profile as well.…”

Section: Introductionmentioning

confidence: 77%

“…Since there were no clinical signs of any inflammatory process or plaque accumulation after restoration either in our or previous studies 29,25,26 inflammatory mediator increase could be attributed to a healing or reparation process. Calcium hydroxide and mineral trioxide aggregate (MTA) are known to stimulate dentinogenesis and cementogenesis, together with early inflammation 79 , while MTA, at least in vitro, demonstrated significant IL-1β stimulating capacity 80 Hydroxyl ions derived from these restorative materials change oxido/reductive balance at lesion site 28 , ultimately inducing chemical tissue irritation and cellular necrosis. Necrotic cells release low levels of cytokines and other damage signals to facilitate the removal of the dead or dying cells, leading to a inflammation without microorganisms in lesion itself 81,82 So, the significant presence of inflammatory mediators in GCF of restored teeth without signs of the inflammatory process could be associated with the reparative process.…”

Section: Discussionmentioning

confidence: 99%

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The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

et al. 2021

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Background/Aim.To estimate the concentrations of the proinflammatory cytokines (IL-1β TNF-in the gingival crevicular fluid (GCF) samples from caries affected teeth and intact teeth, and to correlate them with caries extension, tooth position, and different restorative biomaterials. Methods.GCF samples were collected from the 90 periodontally healthy patients demonstrating at least one tooth with proximal caries and one intact tooth, at the baseline, 7 and 30 days post-treatment. The biomarkers profile was investigated in relation to the different level of caries extension (superficial, pulpitis, gangrenous, root affection), defect size and restorative biomaterial. Results. Before therapy, caries level was significantly associated with GCF IL-1β concentration, demonstrating lowest level in gangrenous (C4) and superficial caries (C2). 30d after therapy, root affection (C5) was characterized by the highest IL-1β concentration. Different dental fillings showed various GCF cytokine change. CPC induced a significant IL-1β increase in more than 70% of treated patients. Caries lesion size was insignificantly associated with GCF levels of these proinflammatory cytokines, where larger defects were followed with average cytokine increase. Considering tooth position, before therapy, IL-1β had the highest level in GCF samples from caries affected canines and second molars while TNF-α showed highest levels from canines GCF. Dental restoration induced cytokine increase in canines (IL-1β and TNF-α), 1st and 2nd molars GCF (IL-1β). Conclusion. Before inflammation intensity of tooth structures was directly reflected with IL-1β and TNF-α concentration. Dental restoration significantly affects IL-1β and TNF-α depending on the used dental filling-type material. Profile of these cytokines varied in GCF samples of the tooth with different anatomical position, where canines and molars demonstrated the highest level. An increase of these proinflammatory cytokines in the absence of any symptomatic manifestation of inflammatory response has to be reconsidered as a possible tooth reparation parametar. Clinical relevance. The concentration of GCF cytokines is illustrative in depicting processes in tooth structures. Their relevance must be inspected with aspects of tooth position and caries lesion level. Different impact of dental restoration materials on GCF IL-1β and TNF-αcould be used as parameter for estimating local inflammation.

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

et al. 2021

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“…On the day of the assays, saliva and GCF were slowly thawed at 4 • C. For GCF extraction, PerioPaper Strips ® (Oraflow, New York, NY, USA) were placed in Eppendorf ® (Eppendorf, Warszawa, Polska) tubes containing 0.02 M phosphate-buffered saline (PBS) (1 strip/500 µL PBS pH 7.0). The samples were mixed for 30 s in a vortex mixer and then centrifuged (4 • C, 3000× g, 5 min) [12,28].…”

Section: Redox Assaysmentioning

confidence: 99%

Total Oxidant and Antioxidant Capacity of Gingival Crevicular Fluid and Saliva in Patients with Periodontitis: Review and Clinical Study

2020

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Periodontitis is inextricably linked to oxidative-reductive (redox) imbalance. However, little is still known about the resultant ability to scavenge oxygen free radicals in saliva and gingival crevicular fluid in patients with periodontitis. The multitude of enzymatic and non-enzymatic antioxidants and their synergistic effects cause an interest in the evaluation of the total antioxidative capacity. Thus, our study aimed to evaluate the total oxidative and antioxidative activity of gingival crevicular fluid and saliva in the periodontitis, as well as to relate these biomarkers to clinical indices of periodontopathy. Additionally, by calculating the oxidative stress index (OSI), the intensity of redox disturbances was also evaluated. Fifty-eight periodontitis patients were included in the study and divided into two subgroups depending on the severity of the disease. In the non-stimulated/stimulated saliva as well as a gingival crevicular fluid of the study group, we found significantly higher OSI and total oxidant status (TOS) as well as lower total antioxidant capacity (TAC). However, the ability to reduce iron ions (FRAP) was significantly lower only in stimulated and non-stimulated saliva of patients with periodontitis. The examined parameters correlated with the periodontium’s clinical condition, which indicates the exacerbation of the inflammatory process. However, TAC, TOS, OSI, and FRAP did not differentiate individual stages of periodontitis.

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“…On the day of determining, the samples containing saliva and gingival crevicular fluid were slowly thawed at 4 • C. In order to extract gingival crevicular fluid, PerioPaper Strips ® were placed in an Eppendorf ® test tube containing 0.02 M phosphate-buffered saline solution (PBS) with pH of 7.0 (1 PerioPaper Strips ® /500 µL PBS). Samples were mixed for 30 s with a vortex mixer and then centrifuged (4 • C, 3000× g, 5 min) [20]. The supernatant fluid was preserved for testing.…”

Section: Redox Assaysmentioning

confidence: 99%

Nitrosative Stress Biomarkers in the Non-Stimulated and Stimulated Saliva, as well as Gingival Crevicular Fluid of Patients with Periodontitis: Review and Clinical Study

2020

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Diagnosis of periodontopathy is complex and includes defining the cause, type, stage, and grade of periodontitis. Therefore, alternative diagnostic methods are sought to indicate the progression of inflammation or to determine the effectiveness of therapy. Gingival crevicular fluid (GCF) biomarkers can be particularly useful because they most likely reflect the disease process of the periodontal tissues. However, the difficulty of collecting GCF for testing is the reason for the limited use in diagnostics. Because periodontitis is the primary source of nitrogen free radicals in the oral cavity, the aim of the study was to evaluate the biomarkers of nitrosative stress (nitric oxide, peroxynitrite, and S-nitrosothiols) in GCF, non-stimulated and stimulated saliva of 90 patients with periodontitis. The study group was divided into two subgroups, depending on the stage of the disease severity. We showed a significantly higher concentration of all assessed biomarkers in the non-stimulated and stimulated saliva of patients with periodontitis. However, significant changes in GCF has been shown only for peroxynitrite. The studied biomarkers did not correlate with clinical periodontal status, which probably results from their short-duration activity and the impact on a few factors in the oral cavity. Saliva and gingival fluid are not very useful in the differential diagnosis of periodontitis.

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Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid

Cited by 10 publications

References 35 publications

The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid

Total Oxidant and Antioxidant Capacity of Gingival Crevicular Fluid and Saliva in Patients with Periodontitis: Review and Clinical Study

Nitrosative Stress Biomarkers in the Non-Stimulated and Stimulated Saliva, as well as Gingival Crevicular Fluid of Patients with Periodontitis: Review and Clinical Study

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