Influence of dietary carotenoids on radical scavenging capacity of the skin and skin lipids

Meinke, Martina C.; Friedrich, Annette; Tscherch, Kathrin; Haag, Stefan; Darvin, Maxim E.; Vollert, Henning; Groth, Norbert; Lademann, Juergen; Rohn, Sascha

doi:10.1016/j.ejpb.2012.11.012

Cited by 85 publications

(81 citation statements)

References 49 publications

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“…In addition to resonance Raman spectroscopy, which is usually applied for detecting the carotenoid concentration in the skin (Figure 3a), EPR spectroscopy was used for measuring the antioxidant status in the skin (Figure 3b). Data taken from visits 1 to 4 (V1, before intake; V2, four weeks after intake; V3, eight weeks after intake; V4, several weeks after discontinuing the intake; (a) relative carotenoid concentration; (b) relative rate constant [38], * p < 0.05, ** p < 0.01, *** p < 0.001.…”

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

“…The placebo control group showed no change in their antioxidant activity, indicating a clear improvement of the radical scavenging activity of the skin in the group with the additional uptake of vitamin C. Further to the in vivo measurements, the radical scavenging capacity of the administered substances, described as radical protection factor (RPF), were investigated by in vitro EPR [55]. The RPF can be determined by the reaction of the test substance with the radical probe 2,2-diphenyl-1-picrylhydrazyl (DPPH) and is defined as 3600 × 10 14 (neutralized radicals)/mg (test substance) [38]. This procedure is a good measure for evaluating the antioxidant potency of a preparation.…”

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

“…Carotenoids such as most of the known antioxidants have to be taken up with food, such as fruits and vegetables, because they cannot be produced by the body itself [60,61]. To investigate the effect of lipophilic antioxidants on the spin probe TEMPO, a double-blinded placebo-controlled study with 24 healthy volunteers was carried out, all of which were given a supplementary lipophilic curly kale extract (Lutex Skin) for 8 weeks [38]. In addition to resonance Raman spectroscopy, which is usually applied for detecting the carotenoid concentration in the skin (Figure 3a), EPR spectroscopy was used for measuring the antioxidant status in the skin (Figure 3b).…”

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

“…A high rate constant represents a high antioxidant capacity of the skin. A comparison between the baseline measurement, prior and subsequent to the topical application or oral intake of antioxidants, provides an insight into the radical scavenging capacity of the skin and how the intake of antioxidants have improved the rate constant [27,32,35,38,39]. To carry out oxidative stress measurements, the stable spin probe 3-(carboxy)-2,2,5,5-tetramethylpyrrolidin-1-oxyl (PCA) is applied onto the skin.…”

Section: Technical Aspects Of the Performance Of In Vivo Epr-measuremmentioning

confidence: 99%

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Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

et al. 2015

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Organisms produce free radicals which are essential for various metabolic processes (enzymatic oxidation, cellular respiration, signaling). Antioxidants are important chemical compounds that specifically prevent the oxidation of substances by scavenging radicals, especially reactive oxygen species (ROS). Made up of one or two unpaired electrons, ROS are free radicals that are highly reactive and can attack other metabolites. By using electron paramagnetic resonance (EPR) spectroscopy, it is possible to measure paramagnetic substances such as free radicals. Therefore the dermal antioxidant activity can be determined by applying semi-stable radicals onto the skin and measuring the antioxidant-induced radical scavenging activity in the skin. In recent years, EPR has been OPEN ACCESS Cosmetics 2015, 2 287 developed as a spectroscopic method for determining the antioxidant status in vivo. Several studies have shown that an additional uptake of dietary supplements, such as carotenoids or vitamin C in physiological concentrations, provide a protective effect against free radicals. Using the EPR technique it could be demonstrated that the radical production in stress situations, such as irradiation with infrared and visible light, was reduced with time. However, not only the oral uptake of antioxidants, but also the topical application of antioxidants, e.g., a hyperforin-rich cream, is very useful against the development of oxidative stress. Regular application of a hyperforin-rich cream reduced radical formation. The skin lipids, which are very important for the barrier function of the skin, were also stabilized.

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Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

Section: Technical Aspects Of the Performance Of In Vivo Epr-measuremmentioning

confidence: 99%

See 2 more Smart Citations

Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

et al. 2015

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“…It has been shown in previous investigations that carotenoids can be measured as marker substance for the whole antioxidant status of the skin, since the antioxidant substances in the skin form protective chains that work against the destructive effects of free radicals [24,25]. Based on carotenoid absorption maxima, which lies in the blue-green range of the spectra, the excitation laser radiation was set at 488 and 514 nm on the skin [26].…”

Section: Resonance Raman Spectroscopymentioning

confidence: 99%

Changes of the skin barrier and bacterial colonization after hair removal by clipper and by razor

Jung

Richter

Darvin

et al. 2016

JBPE

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Abstract. Background: Inappropriate hair removal increases the risk of surgical site infections which are associated with a higher morbidity and mortality of surgical patients. Here, the effects of a clipping device and a disposable razor on the skin barrier, microbial burden and surface structure were compared. Methods: Changes in bacterial colonization, transepidermal water loss, antioxidant status and the skin surface structure were investigated on the calves of 12 healthy volunteers. Measurement time points were at baseline (tbase) and 24 hours after hair removal (t24). Results: Both, the disposable razor and the clipper showed a decrease in log colony-forming units count from tbase (mean(tbase) ± standard deviation = 2.6 ± 1.27, median ± standard error = 2.6 ± 0.37) to t24 at prazo r= 0.05 and pclipper = 0.06 respectively. At t24 clipping resulted in a higher reduction of log colony-forming units (mean(t24) = 1.76 ± 0.8, median = 1.69 ± 0.23) compared to the use of the disposable razor (mean(t24) = 1.84 ± 0.85, median = 1.91 ± 0.24). Furthermore, the razor-treated group showed an increase in colony-forming units from t0 to t24, whereas clipping lead to a continuous decrease in colony-forming units from t0 to t24. An enhanced appearance of microlesions and a significant increase of transepidermal water loss after shaving using the disposable razor (p = 0.005) were found indicating skin barrier disruptions. Clipping showed no significant effect on transepidermal water loss. Conclusion: Hair removal using the clipping device results in less disruption of the skin barrier compared to the razor, avoiding the development of microlesions. This could be favorable for the prevention of surgical side infections and postoperative wound management. © 2016 Journal of Biomedical Photonics & Engineering.Keywords: preoperative hair removal, skin barrier disruption, post-operative, postsurgical infection, clipping.

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Carotenoids

Kaczor¹,

Barańska²,

Czamara³

2016

Carotenoids

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Influence of dietary carotenoids on radical scavenging capacity of the skin and skin lipids

Cited by 85 publications

References 49 publications

Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

Changes of the skin barrier and bacterial colonization after hair removal by clipper and by razor

Carotenoids

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