Influence of Different Parameters on a Dual-Fractal Analysis for Antigen–Antibody Binding Kinetics for Biosensor Applications

Milum, Jeremy; Sadana, Ajit

doi:10.1006/jcis.1996.4614

Cited by 28 publications

(16 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An analysis of the binding kinetics of the antigen in solution to antibody immobilized on the biosensor surface is available (39). The influence of lateral interactions on the surface and variable rate coefficients is also available (32).…”

Section: Theorymentioning

confidence: 99%

“…These investigators noted that for an increasing binding rate coefficient, after a brief time interval, as time increases, the concentration of the antigen near the surface decreases, as expected for the cases when lateral interactions are present or absent. The diffusion-limited binding kinetics of antigen (or antibody or substrate) in solution to antibody (or antigen or enzyme) immobilized on a biosensor surface has been analyzed within a fractal framework (38,39). Furthermore, experimental data presented for the binding of human immunodeficiency virus (HIV) (antigen) to the antibody anti-HIV immobilized on a surface show a characteristic ordered "disorder" (42).…”

Section: Variable Binding Rate Coefficientmentioning

confidence: 99%

“…These authors noted that the prefactor (in our case, the binding or the dissociation rate coefficient) displays uniform trends with the fractal dimension, D f . Fractal dimension values for the kinetics of antigen-antibody binding (38,39), analytereceptor binding (34), and analyte-receptor binding and dissociation (6,27) are available. We would like to further extend these ideas now to two cases: binding and dissociation phase(s), and the dissociation phase alone as well.…”

mentioning

confidence: 99%

See 2 more Smart Citations

A Kinetic Study of Analyte–Receptor Binding and Dissociation, and Dissociation Alone, for Biosensor Applications: A Fractal Analysis

Sadana

2001

Analytical Biochemistry

Self Cite

View full text Add to dashboard Cite

Section: Theorymentioning

confidence: 99%

Section: Variable Binding Rate Coefficientmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

A Kinetic Study of Analyte–Receptor Binding and Dissociation, and Dissociation Alone, for Biosensor Applications: A Fractal Analysis

Sadana

2001

Analytical Biochemistry

Self Cite

View full text Add to dashboard Cite

“…An analysis of the binding kinetics of the antigen in solution to antibody immobilized on the biosensor surface is available (37). The influence of lateral interactions on the surface and variable rate coefficients is also available (40).…”

Section: Theorymentioning

confidence: 99%

“…Fractal dimension values for the kinetics of antigen-antibody binding (36,37) and analyte-receptor binding (38) are available. A fractal analysis of analyte-receptor binding kinetics for SPR applications is available (39).…”

Section: Introductionmentioning

confidence: 99%

A Predictive Approach Using Fractal Analysis for Analyte–Receptor Binding and Dissociation Kinetics for Surface Plasmon Resonance Biosensor Applications

Ramakrishnan

Sadana

2000

Journal of Colloid and Interface Science

Self Cite

View full text Add to dashboard Cite

Interferometric silicon biochips for label and label‐free DNA and protein microarrays

et al. 2012

View full text Add to dashboard Cite

Protein and DNA microarrays hold the promise to revolutionize the field of molecular diagnostics. Traditional microarray applications employ labeled detection strategies based on the use of fluorescent and chemiluminescent secondary antibodies. However, the development of high throughput, sensitive, label-free detection techniques is attracting attention as they do not require labeled reactants and provide quantitative information on binding kinetics. In this article, we will provide an overview of the recent author's work in label and label-free sensing platforms employing silicon/silicon oxide (Si/SiO(2)) substrates for interferometric and/or fluorescence detection of microarrays. The review will focus on applications of Si/SiO(2) with controlled oxide layers to (i) enhance the fluorescence intensity by optical interferences, (ii) quantify with sub-nanometer accuracy the axial locations of fluorophore-labeled probes tethered to the surface, and (iii) detect protein-protein interactions label free. Different methods of biofunctionalization of the sensing surface will be discussed. In particular, organosilanization reactions for monodimensional coatings and polymeric coatings will be extensively reviewed. Finally, the importance of calibration of protein microarrays through the dual use of labeled and label-free detection schemes on the same chip will be illustrated.

show abstract

Influence of Different Parameters on a Dual-Fractal Analysis for Antigen–Antibody Binding Kinetics for Biosensor Applications

Cited by 28 publications

References 19 publications

A Kinetic Study of Analyte–Receptor Binding and Dissociation, and Dissociation Alone, for Biosensor Applications: A Fractal Analysis

A Kinetic Study of Analyte–Receptor Binding and Dissociation, and Dissociation Alone, for Biosensor Applications: A Fractal Analysis

A Predictive Approach Using Fractal Analysis for Analyte–Receptor Binding and Dissociation Kinetics for Surface Plasmon Resonance Biosensor Applications

Interferometric silicon biochips for label and label‐free DNA and protein microarrays

Contact Info

Product

Resources

About