Influence of different sterilization processes on the properties of commercial poly(lactic acid)

Savaris, Michele; Santos, Venina dos; Brandalise, Rosmary Nichele

doi:10.1016/j.msec.2016.07.031

Cited by 68 publications

(70 citation statements)

References 39 publications

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“…Changes in PLA film color from transparent to matte (whitish) were also observed in another study of the group by colorimetric analysis [17]. According to Fukushima et al [38], this change of color is assigned to PLA degradation, which changes the refractive index of the sample, which occurs as a consequence of water absorption and/or the presence of products resulting from the hydrolysis process.…”

Section: Resultsmentioning

confidence: 72%

“…After hygienization, the PLA films were sterilized with ethylene oxide following the mechanical aeration process, hyperventilation, and natural aeration for 24 hours (in an accredited company in the city of Caxias do Sul, Brazil). The hygienization and sterilization parameters used in this study were also applied in other studies of the group [16,17]. According to the FDA (1978), aeration for 96 h at room temperature is recommended for all polymers, except for poly(vinyl chloride) (PVC).…”

Section: Methodsmentioning

confidence: 99%

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Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Savaris

Braga

Santos

et al. 2017

International Journal of Polymer Science

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Biomaterials must meet certain fundamental requirements for their usage in living beings, such as biocompatibility, bifunctionality, and sterilizability, without having chemical and structural changes. The biocompatibility of poly(lactic acid) (PLA) films, shaped by compression, was evaluated after sterilization by ethylene oxide by a histological in vivo test with Wistar rats and cytotoxicity in cell adhesion in vitro. The cytotoxicity test was performed by the reduction of tetrazolium salt (MTT). Thermal and chemical changes in PLA films concerning the proposed sterilization process and characteristics were not observed to evidence polymer degradation due to sterilization. The analysis of the cytotoxicity by the MTT method has shown that the sterilized PLA films are not cytotoxic. The adhesion and proliferation of fibroblasts on PLA films were homogeneously distributed over the evaluation period, showing an elongated appearance with unnumbered cytoplasmic extensions and cell-cell interactions. By examining the biocompatibility in a histological study, a mild tissue inflammation was observed with the presence of fibrosis in the samples that had been exposed for 21 days in the rats' bodies. PLA films sterilized with ethylene oxide did not exhibit cell adhesion in vitro and toxicity to the surrounding tissue in vivo and they may be used in future in vivo testing, according to histological findings in Wistar rats in the present study.

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Section: Resultsmentioning

confidence: 72%

Section: Methodsmentioning

confidence: 99%

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Savaris

Braga

Santos

et al. 2017

International Journal of Polymer Science

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“…Bioburden in and on the device should be reduced practically to zero in order to assure its microbiological safety for the patient. Medical implants made of PLA are typically sterilized by ethylene oxide (EO); however, other low temperature methods such as H 2 O 2 cold plasma may also be considered . These methods are perfectly suited for sterilization of bulky solid devices, nevertheless a certain degree of caution should be taken because the sterilizing agent, EO or hydrogen peroxide, is effective only at the surface.…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Mechanical Properties of FDM‐PLA Parts via Thermal Annealing

Wach

Wolszczak

Adamus-Włodarczyk

2018

Macro Materials & Eng

172

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The objective of this study is to investigate the possibility of enhancing mechanical properties of poly(lactic acid) (PLA) samples processed by a rapid manufacturing (RM) technique by increasing PLA crystallinity degree via thermal annealing. The samples are manufactured by fused deposition modeling (FDM) at different temperatures and subsequently evaluated by three‐point bending flexural and tensile tests. The polymer processed at 215 °C is thermally annealed over its glass transition temperature in order to increase the degree of crystallinity to the maximum attainable level as measured by the differential scanning calorimetry and confirmed by X‐ray diffraction. The increase in the degree of crystallinity of FDM‐PLA enhances flexural stress of the samples by 11–17%. The study also demonstrates applicability of radiation sterilization for FDM‐PLA parts. Therefore, thermal annealing might be introduced into a standard RM technology of PLA, particularly for sterilizable customized implants, to efficiently improve their mechanical properties.

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“…Enzymes that are present at inflamed wound sites and found in plasma, by oxidation or hydrolysis, can affect the degradation rate of the material and, thus, influence the release profile from the device. Also, sterilisation methods, as part of in vivo procedures and regulations can affect the material degradation (Savaris et al, 2016) and bioactivity of the incorporated growth factors (Mainil-Varlet et al, 1997;Moioli et al, 2006). Furthermore, the stability and bioactivity of the released growth factor at the wound site can differ from the in vitro conditions.…”

Section: Part B: Effects Of Pdgf-bb Delivery In Vivomentioning

confidence: 99%

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

Evrova

Buschmann

2017

eCM

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To promote and support tendon healing, one viable strategy is the use or administration of growth factors at the wound/rupture site. Platelet derived growth factor-BB (PDGF-BB), together with other growth factors, is secreted by platelets after injury. PDGF-BB promotes mitogenesis and angiogenesis, which could accelerate tendon healing. Therefore, in vitro studies with PDGF-BB have been performed to determine its effect on tenocytes and tenoblasts. Moreover, accurate and sophisticated drug delivery devices, aiming for a sustained release of PDGF-BB, have been developed, either by using heparin-binding and fibrin-based matrices or different electrospinning techniques.In this review, the structure and composition, as well as the healing process of tendons, are described. Part A deals with in vitro studies. They focus on the multiple effects evoked by PDGF-BB on the cellular level. Moreover, they address strategies for the sustained delivery of PDGF-BB. Part B focuses on animal models used to test different delivery strategies for PDGF-BB, in the context of tendon reconstruction. These studies showed that dosage and timing of PDGF-BB application are the most important factors for deciding which delivery device should be applied for a specific tendon laceration.

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Influence of different sterilization processes on the properties of commercial poly(lactic acid)

Cited by 68 publications

References 39 publications

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Biocompatibility Assessment of Poly(lactic acid) Films after Sterilization with Ethylene Oxide in Histological Study In Vivo with Wistar Rats and Cellular Adhesion of Fibroblasts In Vitro

Enhancement of Mechanical Properties of FDM‐PLA Parts via Thermal Annealing

In vitro and in vivo effects of PDGF-BB delivery strategies on tendon healing: a review

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