Influence of Different Storage Conditions on the Mycotoxin Production and Quality of Fusarium-Infected Wheat Grain

Homdork, S.; Fehrmann, H.; Beck, R. H.

doi:10.1111/j.1439-0434.2000.tb04618.x

Cited by 53 publications

(19 citation statements)

References 14 publications

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“…The DON content found in wheat kernels after harvest usually is highly dependent on Fusarium head blight severity (Visconti et al . 1986; Wang and Miller 1988; Homdork et al . 2000a; Miedaner et al .…”

Section: Discussionmentioning

confidence: 99%

Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat-samples

Schnerr

Vogel

Niessen

2002

Lett Appl Microbiol

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Aims: Correlations between DNA content of trichothecene-producing Fusarium spp. and concentration of the key mycotoxin deoxynivalenol (DON) in cereal samples. Methods and Results: A LightCyclerÔ PCR-based assay was used to quantify the DNA from trichothecene-producing Fusarium spp. in 300 wheat samples. DNA concentrations ranged from not detectable to 16AE3 mg kg )1 whereas DON concentrations (GC/MS data) varied from not detectable to 34AE3 mg kg )1 . Data analysis revealed a coefficient of correlation r ¼ 0AE9557 between DON concentrations and DNA-amounts over all samples. An interval of confidence for P ¼ 95% was calculated based on samples with DON concentrations £ 1AE5 mg kg )1 (n ¼ 234). Conclusions: Quantification of 32 samples of Fusarium-contaminated wheat was performed within 45 min. Data analysis allowed estimation of DON contamination from quantitative PCR data in the wheat samples. Significance and Impact of the Study: The method described is useful for the screening of cereals in industrial quality control.

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Section: Discussionmentioning

confidence: 99%

Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat-samples

Schnerr

Vogel

Niessen

2002

Lett Appl Microbiol

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“…The pathogens deposit multiple mycotoxins in grain during the course of disease development in the crop field and during post-harvest storage [2, 3]. Importantly, the fungal toxins often enter the food chain [4], and exposures to them through consumption may cause serious mycotoxicosis in humans and animals [5–8].…”

Section: Introductionmentioning

confidence: 99%

Microbial detoxification of eleven food and feed contaminating trichothecene mycotoxins

et al. 2017

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BackgroundContamination of agricultural commodities with multiple trichothecene mycotoxins, produced by toxigenic Fusarium species, is a food safety issue, which greatly affects grain production and marketing worldwide. Importantly, exposure to multiple trichothecenes may increase toxicity in animals due to their synergistic and/or additive effects. To address the problem this study aimed to achieve a novel biological trait capable of detoxifying various food and feed contaminating trichothecenes under aerobic and anaerobic conditions and wide range of temperatures.ResultsA highly enriched microbial consortium (called DX100) capable of transforming eleven trichothecenes to significantly less toxic de-epoxy forms was achieved after prolonged incubation of soil microbial culture with 200 μg/mL deoxynivalenol (DON). DX100 demonstrated de-epoxidation activity under aerobic and anaerobic conditions, a greater range of temperatures and around neutral pH. The consortium contains 70% known and 30% unknown bacterial species, dominated by Stenotrophomonas species. Probably novel bacteria including strains of Stenotrophomonas and Alkaliphilus-Blautia species complex could be involved in aerobic and anaerobic de-epoxidation of trichothecenes, respectively. DX100 showed rapid and stable activity by de-epoxidizing 100% of 50 μg/mL deoxynivalenol at 48 h of incubation and retaining de-epoxidation ability after 100 subcultures in mineral salts broth (MSB). It was able to de-epoxidize high concentration of DON (500 μg/mL), and transformed ten more food contaminating trichothecenes into de-epoxy forms and/or other known/unknown compounds. Microbial de-epoxidation rate increased with increasing trichothecene concentrations in the broth media, suggesting that DX100 maintains a robust trichothecene detoxifying mechanism. Furthermore, the nature of microbial de-epoxidation reaction and inhibition of the reaction by sodium azide and the finding that bacterial cell culture lysate retained activity suggests that certain cytoplasmic reductases may be responsible for the de-epoxidation activity.ConclusionsThis study reports the enrichment procedure for obtaining an effective and stable microbial consortium DX100 capable of de-epoxidizing several food contaminating trichothecene mycotoxins. DX100, which has de-epoxidation ability under wide range of conditions, represents a unique enzymatic source which has great industrial potential for reducing contamination of foods/feeds with multiple trichothecenes, and minimizing their synergistic/additive cytotoxic effects on consumer health.

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“…Tebuconazole is a widely used fungicide in agriculture,1–4 mainly in cereal cultivation and on foliage. In general, pesticides can be degraded through abiotic reactions such as photolysis, oxidation and reduction processes.…”

Section: Introductionmentioning

confidence: 99%

High‐performance liquid chromatographic/tandem mass spectrometric identification of the phototransformation products of tebuconazole on titanium dioxide

et al. 2002

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Tebuconazole is a widely used fungicide. The formation of by-products on irradiated titanium dioxide as a photocatalyst was evaluated. Several species derived from tebuconazole degradation were identified and characterized by HPLC/MS(n). A pattern of reactions accounting for the observed intermediates is proposed. Different parallel pathways are operating (and through these pathways the transformation of the molecule proceeds), leading to a wide range of intermediate compounds. All these molecules are more hydrophylic than tebuconazole. The main steps involved are (1) the hydroxylation of the molecule with the formation of three species having [M + H](+) 324; the hydroxylation occurs on the C-1 carbon and on the aromatic ring in the two ortho-positions; (2) the cleavage of a C--C bond with the release of the tert-butyl moiety and the formation of a species having m/z 250; analogously to step 1, also on this species a further hydroxylation reaction occurs; (3) through the loss of the triazole moiety with the formation of a structure with m/z 257.

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Influence of Different Storage Conditions on the Mycotoxin Production and Quality of Fusarium-Infected Wheat Grain

Cited by 53 publications

References 14 publications

Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat-samples

Correlation between DNA of trichothecene-producing Fusarium species and deoxynivalenol concentrations in wheat-samples

Microbial detoxification of eleven food and feed contaminating trichothecene mycotoxins

High‐performance liquid chromatographic/tandem mass spectrometric identification of the phototransformation products of tebuconazole on titanium dioxide

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