Influence of Genotype, Plant Growth Temperature and Anther Incubation Temperature on Microspore Embryo Production in<i>Brassica napus ssp. oleifera</i>

Dunwell, J. M.; Cornish, M A; Courcel, A. G. L. De

doi:10.1093/jxb/36.4.679

Cited by 59 publications

(23 citation statements)

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“…Haploid plants can be produced by culturing anthers or isolated microspores of rapeseed (Brassica napus) (3,9,12,13). Microspore to haploid embryo conversion frequencies as high as 10 to 50% have been reported (9,16), but for most genotypes <1% of the microspores that are cultured will develop into embryos.…”

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confidence: 99%

“…Seasonal variations in anther response were reported for a number of species including Solanum tuberosum (4), Hordeum vulgare (2), and Triticum aestivum (17). For B. napus, controlled studies of the effects of growth temperature, light intensity, and light duration on anther culture response showed that embryo production could be significantly increased by growing the donor plants at a low (1 5C) temperature (3,11,12) and high light (12,20). There is no detailed report of the 'Supported by the Ontario Ministry of Agriculture and Food.…”

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confidence: 99%

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Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Pauls²

1992

Plant Physiol.

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The influence of donor plant growth conditions on microspore embryogenesis in rapeseed (Brassica napus) was metric measurements of the microspores isolated from 2.5-to 5.0-millimeter buds showed that the microspores isolated from low-temperature-grown plants had significantly lower log 90-degree light scatter to forward angle light scatter and log 90-degree light scatter to time of flight ratios than those isolated from high-temperature-grown plants, suggesting that the former are more translucent than the latter. Thus, the effect of donor plant growth temperature on microspore embryogenesis may be mediated by a change in the physiology of the microspore cell, which results in the reduction of its cytoplasmic granularity and/ or exine density.Haploid plants can be produced by culturing anthers or isolated microspores of rapeseed (Brassica napus) (3, 9, 12, 13). Microspore to haploid embryo conversion frequencies as high as 10 to 50% have been reported (9, 16), but for most genotypes <1% of the microspores that are cultured will develop into embryos.Previous research with several plant species has shown that the conditions under which the donor plants were grown affect the frequency of androgenesis in culture. Seasonal variations in anther response were reported for a number of species including Solanum tuberosum (4), Hordeum vulgare (2), and Triticum aestivum (17). For B. napus, controlled studies of the effects of growth temperature, light intensity, and light duration on anther culture response showed that embryo production could be significantly increased by growing the donor plants at a low (1 5C) temperature (3,11,12) and high light (12,20). There is no detailed report of the 'Supported by the Ontario Ministry of Agriculture and Food.

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confidence: 99%

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Pauls²

1992

Plant Physiol.

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“…Low temperature pretreatment improves microspore embryogenesis in B. napus (Lichter 1982), B. juncea (George and Rao 1982), and B. oleracea (Osolnik et al 1993). However, low temperature treatment of the buds inhibits microspore embryogenesis in B. campestris (Keller et al 1982) and B. napus (Dunwell et al 1985). Our results showed that in the microspore culture of B. rapa (syn.…”

Section: Discussionmentioning

confidence: 60%

“…These contradictory results might have been caused by the difference in the conditions of the treatment. In the cases of negative effects reported, buds were put in a sealed bottle (Dunwell et al 1985) or a sealed plastic bag (Keller et al 1982) during the pretreatment. However, in the studies where promotive effects were obtained, buds were put on a piece of wet cotton (Osolnik et al 1993) or in a liquid medium (Lichter 1982) during the pretreatment.…”

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confidence: 99%

Effect of Low Temperature Pretreatment of Buds or Inflorescence on Isolated Microspore Culture in Brassica rapa(syn. B.campestris).

et al. 2002

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The effect of low temperature pretreatment of buds or inflorescence on microspore culture for the production of haploids of Brassica rapa (syn. B.campestris) was examined. Incubation of the buds or the inflorescence at 4°C for 3 or 10 days before the culture of microspores induced efficient microspore embryogenesis. Pretreatment of flower buds was more effective than that of the inflorescence. Prolonged pretreatment up to 20 days promoted embryo induction. Microspores in the buds were examined for developmental stage before and after the pretreatment. Buds with a petal length to anther length ratio of about 0.5-0.7, which had microspores were at the late unicellular stage, were collected and were used. All microspores were at the late unicellular stage before the pretreatment. The percentage of the microspores at the stage of late unicellular decreased during the pretreatment while the percentage of bicellular stage microspores with two unequal size nuclei increased. At the same time, a small but notable number of bicellular stage microspores with equal size nuclei, which is the first step of microspore embryogenesis, was observed after the pretreatment.

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“…[44] used 15/10˚C for light and dark conditions in a plant growth chamber and observed a better performance of microspore culture. Lower growing temperature can support an equal development of the donor plant that can enhance the success of the microspore culture [2,10,26]. Most commonly the donor plants are grown in a growth chamber for microspore culture.…”

Section: Donor Plants Growing Conditionmentioning

confidence: 99%

Behind the scenes of microspore-based double haploid development in Brassica napus: A review

Rahman¹,

Jiménez²

2016

J Plant Sci Mol Breed

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Double haploids are extremely valuable for generating completely homozygous genotypes and have been used in plant breeding program of a number of crop species. This method is a much faster way of developing genetically pure breeding lines in one single generation. The main objective of this review is to describe in a clear and simple manner how microspore-based double haploids of rapeseed are produced and helps a reader to understand the amazing process of microspore embryogenesis, also referred to as androgenesis or pollen embryogenesis. This review will explain what double haploids are as well as their importance in both Brassica breeding and molecular studies. Additionally, a brief discussion of different factors affecting the double haploid production and a comprehensive explanation of the steps involved in the development of the double haploids will be covered.

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Influence of Genotype, Plant Growth Temperature and Anther Incubation Temperature on Microspore Embryo Production inBrassica napus ssp. oleifera

Cited by 59 publications

References 11 publications

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Plant Growth Environment Effects on Rapeseed Microspore Development and Culture

Effect of Low Temperature Pretreatment of Buds or Inflorescence on Isolated Microspore Culture in Brassica rapa(syn. B.campestris).

Behind the scenes of microspore-based double haploid development in Brassica napus: A review

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