Influence of gossypol on the secretory function of cultured rat sertoli cells

Monsees, Thomas K.; Winterstein, U.; Schill, Wolf‐Bernhard; Miska, W.

doi:10.1016/s0041-0101(97)00162-1

Cited by 11 publications

(7 citation statements)

References 18 publications

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“…With the exception of the pesticide DDT, all chemicals analysed in this study enhanced the production of lactate by Sertoli cell cultures at noncytotoxic concentrations. A dose‐dependent increase in lactate secretion was also reported after exposure of Sertoli cells in vitro to a number of reproductive toxicants, including phthalate esters ( Williams & Foster, 1989), nitrobenzene ( Allenby et al ., 1990 ), gossypol ( Monsees et al ., 1998a ) and certain heavy metal ions ( Monsees et al ., 1998b ). Therefore increased lactate production seems to be a sensitive and specific response to toxin exposure, at least for the proven Sertoli cell toxicants 1,3‐dinitrobenzene (1,3‐DNB) and mono‐(2‐ethylhexyl)phthalate (MEHP).…”

Section: Discussionmentioning

confidence: 99%

“…Various toxicants are known to target mainly Sertoli cells. They include chemotherapeutics such as cisplatin ( Wallace et al ., 1989 ), drugs like gossypol ( Monsees et al ., 1998a ), plasticizers such as phthalate esters ( Thomas et al ., 1978 ) or alkylphenols ( De Jager et al ., 1998 ), organic solvents, e.g. hexane, hexanone and dinitrobenzene ( Chapin et al ., 1983 ; Williams & Foster, 1989), pesticides, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Sertoli cells as a target for reproductive hazards

et al. 2000

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Male fertility can be impaired by various toxicants. Some of them are known to target mainly Sertoli cells, which play an essential role in spermatogenesis. In this study, the in vitro response of immature rat Sertoli cells to various environmental pollutants, including pesticides, oestrogenic compounds and heavy metals, has been investigated. Mitochondrial dehydrogenase activity has been used to measure Sertoli cell viability, while production of lactate and secretion of inhibin B have been used as general and specific cell markers. Sertoli cell viability was not affected after 24-h exposure to lindane, DDT, ethinyloestradiol or bisphenol A in the concentration range analysed (up to 100, 25 or 50 microM, respectively). In contrast, mercury(II) (EC50 = 31 microM) and cisplatin (15% decrease in viability at 100 microM) induced some cytotoxic effect. With the exception of the pesticide DDT, all chemicals investigated induced a significant dose-dependent increase in lactate production after 24-h exposure to Sertoli cells. Owing to the cytotoxic effect of mercury(II), lactate levels dropped again at concentrations above 20 microM. The pesticide lindane (but not DDT) and both oestrogens significantly increased the production of the Sertoli cell specific hormone inhibin B without affecting cell viability. In contrast, the heavy metals mercury(II) and platinum(II) markedly decreased inhibin B levels. This sharp decrease was already significant at metal concentrations that reduced Sertoli cell viability only moderately (10-15%). In conclusion, the secretion of lactate and inhibin B by immature rat Sertoli cells seems to be a useful and sensitive marker with which to explore potential Sertoli cell toxicants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sertoli cells as a target for reproductive hazards

et al. 2000

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“…Several toxicants primarily target SCs and impair spermatogenesis by compromising the adhesion between SCs or Sertoli–germ cells. Among those, we can highlight chemotherapeutics (Wallace et al ., ), gossypol (Monsees et al ., ), phthalates (Desdoits‐Lethimonier et al ., ), alkylphenols (Raychoudhury et al ., ), pesticides (Alves et al ., ) and heavy metals (Bizarro et al ., ; Ko et al ., ). Considering the paramount relevance of SCs for the support of developing germ cells, it is clear that any toxicant acting on these cells may potentially disrupt this process.…”

Section: Toxicants Affect Male Reproductive Function By Impairing Sermentioning

confidence: 99%

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

Reis

Moreira

Sousa

et al. 2015

J of Applied Toxicology

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Pressure towards population aging in the demographic pyramid is not only due to sociological/personal choices but also due to subfertility or infertility. There are several chemicals and mixtures that impair male fertility. While experimental animal models are crucial to identify compounds that affect male fertility, it is essential to use reliable in vitro models to determine cellular targets and intracellular pathways that mediate chemical toxicity in the male reproductive system. In this review, we focused on the somatic Sertoli cell (SC) that, within the testis, is a major target for hormonal signaling and provides physical and nutritional support to developing germ cells. The different outcomes possible in each type of study: in vivo versus in vitro (either in primary or immortalized cell cultures) are analyzed. Herein, we intend to clarify the unique features that render SCs as excellent candidates for a robust in vitro model to study the deleterious effects of chemicals on male reproductive health. The sensitivity of SCs to toxicants/pharmaceuticals is discussed and, based on the literature reviewed we propose the in vitro study of SC physiology as a model to disclose deleterious effects of substances to male fertility.

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“…Thereby it undermines the animal spermatogenic epithelium, results in abnormality and death of sperm, or even no sperm. Finally, it causes sire infertility, reduces mitochondrial function and results in infertility (Monsees et al, 1998). Many researches suggests that, apoptosis plays an important role in testis regression (Furuta et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

DNA damage and decrease of cellular oxidase activity in piglet sertoli cells exposed to gossypol

Zhang¹,

Yuan²,

He³

et al. 2011

Afr. J. Biotechnol.

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The study was designated to explore the toxic effects of gossypol on piglet sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet sertoli cells were cultured and classified into five groups, that is, group A, the control without gossypol, group B with 2.5 μg/ml gossypol, group C with 5 μg/ml gossypol, group D with 10 μg/ml gossypol and group E with 20 μg/ml gossypol. We found that sertoli cells' growth was inhibited by gossypol at dose 2.5 μg/ml when compared with the control group. The oxidase activity of sertoli cell also decreased at 2.5 μg/m gossypol. Moreover, DNA damage of sertoli cells was observed at 5 μg/ml gossypol. Putting this into consideration, our study suggests that exposure of gossypol to sertoli cells leads to an inhibition of sertoli cell growth and oxidase activity of sertoli cells at a low concentration, whereas gossypol results in DNA damage of sertoli cells at a higher concentration.

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Influence of gossypol on the secretory function of cultured rat sertoli cells

Cited by 11 publications

References 18 publications

Sertoli cells as a target for reproductive hazards

Sertoli cells as a target for reproductive hazards

Sertoli cell as a model in male reproductive toxicology: Advantages and disadvantages

DNA damage and decrease of cellular oxidase activity in piglet sertoli cells exposed to gossypol

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