Influence of α‐tocopherol, propolis and piperine on therapeutic potential of tiferron against beryllium induced toxic manifestations

Abstract

The therapeutic potential of the chelator tiferron (sodium-4,5-dihydroxy-1,3-benzene disulphonate; 300 mg kg(-1), i.p.) and adjuvants, i.e. alpha-tocopherol (25 mg kg(-1), p.o.), propolis (a honey-bee hive product; 200 mg kg(-1), p.o.) and piperine (10 mg kg(-1), p.o.) were evaluated individually and in combination against beryllium induced biochemical alterations and oxidative stress consequences. Female albino rats were exposed to beryllium nitrate (1 mg kg(-1), i.p.) daily for 28 days followed by treatment with the above mentioned therapeutic agents for 5 consecutive days. Administration of beryllium altered blood biochemical variables with significant depletion in hemoglobin, blood sugar, total serum protein, albumin and significant enhancement in the release of serum transaminases. A significantly increased lipid peroxidation and a decreased level of glutathione after beryllium exposure indicated oxidative stress in the liver and kidney. Beryllium exposure decreased total protein and glycogen contents, whereas triglycerides and cholesterol increased significantly in liver and kidney. Individual administration of all the four compounds showed significant therapeutic potential in reverse of some of the biochemical parameters mentioned above. Furthermore, the combination of tiferron with alpha-tocopherol, propolis or piperine, respectively, could reverse all the variables significantly more towards the control. None of the test compounds showed any significant change in choleretic activity (bile flow and bile solids), indicating that these compounds had no adverse effects at these dose levels. It was concluded that all the combinations of tiferron and adjuvants played a beneficial role in reducing beryllium induced systemic toxicity at relatively lower doses and the combination of tiferron and propolis showed a more pronounced therapeutic potential.

Bioavailability enhancers of herbal origin: An overview

“…They found that the combination of tiferron with piperine could reverse all the variables significantly towards the control [121] .…”

Co-administration of adjuvants along withMoringa oleiferaattenuates beryllium-induced oxidative stress and histopathological alterations in rats

“…Decrease in blood sugar due to beryllium toxicity provides the signal for glycogenolysis thus the level of hepatic glycogen decreases (Agrawal et al, 2013a). Beryllium inhibits the key regulatory enzymes of carbohydrate metabolism (Nirala et al, 2008) thus glucose and its derived product (glucose-6-phosphate) enter into pentose phosphate pathway for the production of body energy and reducing equivalent. The G-6-Pase is a SER membrane enzyme involved in glycogenolysis.…”

“…), and other Piper species (Piperaceae). It has bioavailability enhancing activity for some drugs (Nirala et al, 2007(Nirala et al, , 2008. Piperine is known to exhibit a variety of biological activities including antiinflammatory (Ying et al, 2013), antioxidant (Jain & Mishra, 2011), antithyroid (Vijayakumar & Nalini, 2006), antimutagenic (Srinivasan, 2007), antitumor (Li et al, 2011), antidepressant (Mao et al, 2011), and hepatoprotective activity (Sahu et al, 2012).…”

Safety evaluation of Trikatu, a generic Ayurvedic medicine in Charles Foster rats

“…However, there are reports about the potential pharmacological and toxicological effects of piperine as such and some herbs containing piperine. Role of piperine in reversal of beryllium induced adverse effects was studied in rats and found that piperine at 10 mg/kg body weight was quite effective in the remediation of toxic response (Nirala ., 2008). Similarly, piperine (1, 10 and 50 μg/ml) was found effective in cadmium induced alterations in murine thymocytes pertaining to its anti-oxidative, anti-apoptotic and restorative activity (Pathak and Khandelwal, 2006).…”