Comparison of reheating tensile test (RHT) and re-melting tensile test (RMT) reveals the effect of interdendritic impurity segregation on hot ductility in lowcarbon steels. Two low-carbon steels with different amounts [wt%] of impurity elements (Steel A: P ¼ 0.005, S ¼ 0.001; Steel B: P ¼ 0.01, S ¼ 0.004) are tensiletested at temperatures 600-1000 C after reheating to 1350 C and re-melting at 1570 C. Steel A shows similar hot ductility behavior in the RHT and RMT, whereas the high-impurity steel shows a significant decrease in hot ductility at 900 C in the RMT compared with the RHT. Crack initiation at the interdendritic segregation region is suggested as an origin of the degradation of hot ductility in the high-impurity steel. The effect of interdendritic impurity segregation on the hot ductility behavior of continuously cast steel is further discussed.