Influence of Light, UV-B Radiation, and Herbicides on Wax Biosynthesis of Cucumber Seedling

Tevini, Manfred; Steinmüller, D.

doi:10.1016/s0176-1617(87)80272-9

Cited by 70 publications

(39 citation statements)

References 20 publications

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“…1), early shoot development (Cape & Percy, 1993) and lower shade tolerance relative to other species (Farrar, 1995). Steinmuller & Tevini (1985), Tevini & Steinmuller (1987), with soybean and Barnes et al (1994Barnes et al ( , 1996 with tobacco, observed a shift toward shorter chain lengths with increased u.v.-B. No effect of u.v.-B on short-to long-chain length homologue ratios was recorded in this study.…”

Section: supporting

confidence: 42%

Effects of u.v.‐B radiation on epicuticular wax production and chemical composition of four Picea species

1998

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Two-yr-old Norway (Picea abies (L.) Karst.), red (P. rubens Sarg.), black (P. mariana (Mill.) B.S.P.) and white (P. glauca (Moench.) Voss) spruce seedlings were exposed from bud break for 35 or 63 d (4n5 h irradiance d −" ) to a gradient of biologically effective u.v.-B radiation (λ l 280-315 nm) ranging from 0n61 kJ m −# d −" to 5n99 kJ m −# d −" . No visible symptoms of u.v.-B injury were observed. Epicuticular wax production was not affected by needle exposure to increasing u.v.-B irradiance. Seven constituent classes were resolved by GC and confirmed by GC-MS in wax recovered from needles of the four species. Wax composition of Norway, black and red spruce was altered following needle exposure to increasing u.v.-B dose. White spruce wax composition was unaffected. Direction and magnitude of wax composition response was species-dependent. The proportion of nonacosane diols on Norway spruce needles increased (P 0n05) whereas that of alkyl esters decreased with increasing u.v.-B dose. The proportion of fatty acids in black spruce needle wax increased (P 0n05), and that of estolides (GC-identified) in red spruce needle wax increased (P 0n05) with increasing u.v.-B dose. Changes in wax chemical composition reported were induced following to daily, 4-h duration exposures of needles to u.v.-B centred on 1200 hours. Affected variables exhibited a continuum of response. The highest dose applied was within the range of measured or predicted increases in mid-northern latitudes. Such changes in conifer needle epicuticular wax chemical composition might result in increased seedling sensitivity to the changing atmospheric environment, especially from co-exposure to tropospheric ozone in mid-northern latitudes where much of Canada's productive forest is located.

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Section: supporting

confidence: 42%

Effects of u.v.‐B radiation on epicuticular wax production and chemical composition of four Picea species

1998

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“…In a comparative study using bean, cucumber, and barley leaves of plants subjected to UV-B radiation, the wax layer increased most on barley leaves, followed by bean and cucumber (Steinmiiller and Tevini, 1985). In cucumber, where the main wax components are the alkane-l-ols and alkanes, there was a shift toward shorter-chain homologues with concomitant reduction in long-chain alkanes with enhanced levels of UV -B radiation (Tevini and Steinmiiller, 1987). It was also shown by these authors that the influence ofUV-B radiation was different from that of visible light, making UV -B radiation a potentially important factor in altered wax composition.…”

Section: Effect Of Uv-absorbing Pigments Pubescence and Wax Layermentioning

confidence: 99%

Effects of Ultraviolet-B Radiation on Terrestrial Plants

Bornman

Teramura

1993

Environmental UV Photobiology

192

138

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“…When dark-grown barley seedlings were exposed to light, the rate of wax deposition increased 7.5-fold in the first 24 h of exposure (Giese, 1975). High irradiation also increased total wax loads in Brassica species (Shepherd et al, 1995), and in particular, alkane synthesis in cucumber (Cucumis sativus) seedlings was upregulated in a light-dependent manner (Tevini and Steinmüller, 1987). It was reported that the expression of CER6/KCS6, which encodes a wax-specific 3-ketoacylCoA synthase, is induced by light (Hooker et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

ArabidopsisCuticular Wax Biosynthesis Is Negatively Regulated by theDEWAXGene Encoding an AP2/ERF-Type Transcription Factor

et al. 2014

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The aerial parts of plants are protected from desiccation and other stress by surface cuticular waxes. The total cuticular wax loads and the expression of wax biosynthetic genes are significantly downregulated in Arabidopsis thaliana under dark conditions. We isolated Decrease Wax Biosynthesis (DEWAX), which encodes an AP2/ERF-type transcription factor that is preferentially expressed in the epidermis and induced by darkness. Disruption of DEWAX leads to an increase in total leaf and stem wax loads, and the excess wax phenotype of dewax was restored to wild type levels in complementation lines. Moreover, overexpression of DEWAX resulted in a reduction in total wax loads in leaves and stems compared with the wild type and altered the ultrastructure of cuticular layers. DEWAX negatively regulates the expression of alkane-forming enzyme, long-chain acyl-CoA synthetase, ATP citrate lyase A subunit, enoyl-CoA reductase, and fatty acyl-CoA reductase, and chromatin immunoprecipitation analysis suggested that DEWAX directly interacts with the promoters of wax biosynthesis genes. Cuticular wax biosynthesis is negatively regulated twice a day by the expression of DEWAX, throughout the night and at stomata closing. Significantly higher levels (10-to 100-fold) of DEWAX transcripts were found in leaves than in stems, suggesting that DEWAX-mediated transcriptional repression may be an additional mechanism contributing to the different total wax loads in leaves and stems.

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Influence of Light, UV-B Radiation, and Herbicides on Wax Biosynthesis of Cucumber Seedling

Cited by 70 publications

References 20 publications

Effects of u.v.‐B radiation on epicuticular wax production and chemical composition of four Picea species

Effects of u.v.‐B radiation on epicuticular wax production and chemical composition of four Picea species

Effects of Ultraviolet-B Radiation on Terrestrial Plants

ArabidopsisCuticular Wax Biosynthesis Is Negatively Regulated by theDEWAXGene Encoding an AP2/ERF-Type Transcription Factor

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