Influence of nanophase titania topography on bacterial attachment and metabolism

Abstract

Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity. Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fl uorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism is unclear. Results presented here show that the adhesion of Pseudomonas fl uorescens 5RL and Pseudomonas putida TVA8 was higher on nanophase than conventional titania. Importantly, in terms of metabolism, bacteria attached to the nanophase surfaces had higher bioluminescence rates than on the conventional surfaces under all nutrient conditions. Thus, the results from this study show greater select bacterial metabolism on nanometer than conventional topographies, critical results with strong consequences for the design of improved biosensors for bacteria detection.

Biosynthesis of nano Zinc and using of some nanoparticles in reducing of Cercospora leaf spot disease of sugar beet in the field

“…Oscarpoly in the field. This results were supported by the findings of Palmqvist et al (2015), they found that TiO 2 NPs protected oilseed rape against A. brassicae infection and caused plant growth promoting of roots and increasing of adhesive force between B. amyloliquefaciens and plants by formation protective encapsulating interspecies (Chowdhury et al 2012;Webster et al 2008).…”

“…TiO 2 NPs have become one of the most important substances in nanotechnology which caused plant growth promoting to roots of oilseed rape and protected the plants against Alternaria brassicae infection and adhesive effects on bacteria (Bacillus amyloliquefaciens; Palmqvist et al 2015). TiO 2 NPs is useful as protective encapsulating agents that increase adhesive force in the interspecies relation between bacteria and plants (Chowdhury et al 2012;Webster et al 2008). Ruffolo et al (2010) and Song et al (2014) found that ZnTiO 3 and Zn hydroxide carbonate NPs showed higher growth inhibition efficiency of A. niger and fungal activity against cotton Verticillium, Rhizopus, Mucorales than ZnONPs, respectively.…”

Bioadhesion in the oral cavity and approaches for biofilm management by surface modifications

“…For much smaller nanostructures, the reported effects differ strongly between being high and low adhesive [243,244]. It has been shown that even the smallest differences in surface nanoroughness can strongly affect microbial cells' attachment [245][246][247][248][249][250]. These variations must be connected with certain spacings of bacterial adhesion sites on the membrane or cell appendages, but there is no direct evidence in the literature so far.…”

Enhanced antiadhesive properties of chitosan/hyaluronic acid polyelectrolyte multilayers driven by thermal annealing: Low adherence for mammalian cells and selective decrease in adhesion for Gram-positive bacteria

“…But the topographic characteristics of the surfaces are also expected to affect the adhesion process. This aspect has been considered and carefully studied [65][66][67] Some authors have reported an increase in bacteria adhesion with surface roughness [68,69] while others, the reverse effect [70,71]. In these studies the roughness is estimated with the root mean square of heights (Rq) that can be appropriate for certain surfaces.…”