Influence of nicotine on the adherence of <i>Candida albican</i>s ATCC 14053 and <i>Candida parapsilosis</i> ATCC 22019 and expression of selected binding-related genes

Gunasegar, Shan; Harun, Wan Himratul Aznita Wan

doi:10.1080/13102818.2017.1334593

Cited by 4 publications

(6 citation statements)

References 19 publications

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“…In addition, nicotine from different sources enhanced hyphae formation in C. albicans biofilms (Figure 3). A similar study supported our data that N increased the biofilm formation of C. albicans [32]. In addition, a dose-response effect of CSC on promoting adhesion and biofilm formation of C. albicans as well as the inhibitory impact of CSC at higher concentrations was previously reported [3,22].…”

Section: Discussionsupporting

confidence: 92%

“…In this study, the inhibitory (MIC) and killing (MFC) effects of nicotine from different sources were determined and found to be within the range of nicotine levels present in the saliva of smokers [8,9]. Concentrations of N higher than 4 mg/mL showed an inhibitory effect on C. albicans (Table 1), which was in agreement with previous reports [8,32]. Interestingly, CSC and ECSC produced inhibitory effects against C. albicans at nicotine levels that were about an order of magnitude lower than for N (Table 1).…”

Section: Discussionsupporting

confidence: 90%

“…The HWP1 gene encodes the hyphal wall protein1, which is the major protein of C. albicans involved in hyphal development, cell wall assembly, and intracellular signaling pathways. Notably, overexpression of HWP1 increased the attachment of C. albicans cells to the tooth surface and buccal epithelial cells as the primary stage of colonization and biofilm formation [32,[36][37][38].…”

Section: Discussionmentioning

confidence: 98%

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Effect of Cigarette and E-Cigarette Smoke Condensates on Candida albicans Biofilm Formation and Gene Expression

Haghighi

Andriasian

Tran

et al. 2022

IJERPH

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Smoking triggers environmental changes in the oral cavity and increases the risk of mucosal infections caused by Candida albicans such as oral candidiasis. While cigarette smoke has a significant impact on C. albicans, how e-cigarettes affect this oral pathogen is less clear. Here, we investigated the effect of cigarette and e-cigarette smoke condensates (CSC and ECSC) on C. albicans growth, biofilm formation, and gene expression. Whereas pure nicotine (N) at the minimum inhibitory concentration (MIC, 4 mg/mL) prevented C. albicans growth, enhanced biofilm formation was observed at 0.1 mg/mL. In contrast, at this nicotine sub-MIC (0.1 mg/mL) concentration, CSC and ECSC had no significant effect on C. albicans biofilm formation. Additionally, N, CSC, and ECSC increased the expression of HWP1 and SAP2 genes. The ECSC group exhibited elevated expression levels of the EAP1 and ALS3 genes, compared to the nicotine-free ECSC (−) control. Moreover, our in vitro study illustrated that the antifungal drugs, fluconazole and amphotericin B, alleviated the effect of nicotine on C. albicans gene expression. Overall, the results of the study indicated nicotine from different sources may affect the pathogenic characteristics of C. albicans, including hyphal growth, biofilm formation, and particularly the expression of virulence-related genes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Effect of Cigarette and E-Cigarette Smoke Condensates on Candida albicans Biofilm Formation and Gene Expression

Haghighi

Andriasian

Tran

et al. 2022

IJERPH

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“…The possible mechanism of hydroxychavicol to inhibit the hyphal growth is by (1) altering the cellular morphology which lead to the unproductive cells and (2) delaying germination process by changing the expression of hyphae-related genes such RAS1, NRG1 and HWP1. Our finding strongly suggests that hydroxychavicol is effective against Candida species such C. albicans and this could support its usage in oral rinse to control and prevent candidal infection especially among target users like cigarette smokers [44] and immunocompromised patients [45].…”

Section: Transcriptional Response Of Ras1 Nrg1 and Hwp1 Gene To Hydrsupporting

confidence: 58%

Anti-hyphal properties of potential bioactive compounds for oral rinse in suppression ofCandidagrowth

Mail

Harun

Musa

2017

Biotechnology & Biotechnological Equipment

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Most oral rinses contain active ingredients with limited antifungal activity, particularly against hyphae development. This has encouraged us to search for bioactive compounds effective against preventing morphology transition of the dimorphic Candida. The antifungal activity of the selected bioactive compounds against seven species of oral-associated Candida was screened before the evaluation of their potency to inhibit hyphal formation. The identified potent inhibitor was then further investigated on its effectiveness through a brief treatment test on germination, adhesion, cellular morphology and gene expression. Bakuchiol, hydroxychavciol and pseudolaric acid B showed antifungal activity while luteolin and sakuranetin were determined to be inactive against the oral-associated Candida species at a concentration below 1 mg/mL. Hydroxychavicol was determined as a potent inhibitor against hyphal growth of Candida albicans. Hydroxychavicol delayed the germination process of C. albicans by affecting the expression of RAS1, NRG1 and HWP1 genes up to 1 h after the treatment. In addition, the treatment caused minimal changes to the cellular morphological structure. But, hydroxychavicol showed poor anti-adherence activity against the germinated cells. In summary, hydroxychavicol is one of the identified bioactive compounds that possessed anti-candida properties against oral-associated Candida species and appeared potent in inhibition of hyphae of C. albicans by affecting its ultrastructural morphology and gene regulation. This finding could suggest development of hydroxychavicol as a bioactive compound that can potentiate the activity of oral rinse in preventing the colonization of dimorphic Candida in the oral cavity.

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“…Huang et al 45 found that nicotine stimulates S. gordonii planktonic cell growth, biofilm formation, aggregation, and gene expression translated into binding proteins. Furthermore, Shan et al 46 suggested that nicotine can enhance the growth of Candida albicans (C. albicans) and C. parapsilosisin vitro and influence their adherence to the surface of microplate wells that mimic the tooth surface adherence. These effects may promote later pathogen attachment to tooth surfaces, the accumulation of tooth calculus, and the development of periodontal disease in cigarette smokers.…”

Section: Resultsmentioning

confidence: 99%

Effect of tobacco on periodontal disease and oral cancer

Zhang¹,

He²,

He³

et al. 2019

Tob. Induc. Dis.

111

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INTRODUCTION Periodontal disease and oral cancer are common health hazards. Epidemiological investigations show that smoking, periodontal disease and oral cancer are closely related. Tobacco is one of the major risk factors for periodontitis and oral cancer. METHODS A systematic literature review was performed. To identify relevant studies, the following online databases were searched using specific keywords: PubMed, Web of Science and CNKI. RESULTS Tobacco not only possesses an addictive effect, but it aggravates periodontal disease by promoting the invasion of pathogenic bacteria, inhibiting autoimmune defense, aggravating the inflammatory reaction, and aggravating the loss of alveolar bone. According to current evidence, tobacco significantly aggravates the development and progression of periodontal disease and oral cancer, and periodontal disease may be related to the prevalence of oral cancer. CONCLUSIONS Clinicians should strongly recommend that smokers undertake a strategy to stop smoking to avoid the exacerbation of nicotine-related periodontal disease and to reduce the incidence of oral cancer.

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Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes

Cited by 4 publications

References 19 publications

Effect of Cigarette and E-Cigarette Smoke Condensates on Candida albicans Biofilm Formation and Gene Expression

Effect of Cigarette and E-Cigarette Smoke Condensates on Candida albicans Biofilm Formation and Gene Expression

Anti-hyphal properties of potential bioactive compounds for oral rinse in suppression ofCandidagrowth

Effect of tobacco on periodontal disease and oral cancer

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