Influence of nonlinear effects on the three-photon excitation of L-Tryptophan in water using phase-shaped pulses

Patas, Alexander; Achazi, Georg; Winta, Christopher; Lindinger, Albrecht

doi:10.1364/josab.31.002208

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…The formed laser pulses cause selective multi-photon induced fluorescence of vitamins. Special antisymmetric phase functions will be employed for scans of the multi-photon excitation fluorescence [13]. Application of phase-shaped laser pulses for imaging contrast enhancement is demonstrated for endogeneous vitamin fluorophores in a liquid environment after transmitting a nanostructured kagome fiber.…”

Section: Laser Physicsmentioning

confidence: 99%

Contrast improvement of autofluorescent vitamins by using shaped laser pulses after a kagome fiber

Weber¹,

Otto²,

Lindinger³

2019

Laser Phys.

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An improved two-photon excited fluorescence contrast difference between autofluorescent vitamins is reported by using shaped laser pulses after a kagome fiber. Specifically phasetailored laser pulses are applied for two-photon excited fluorescence of retinol and riboflavin in solution at the distal end of the fiber. The pulse distortions due to the fiber properties are precompensated to achieve predefined tailored pulses after the kagome fiber. A clear twophoton excited fluorescence contrast difference is received between vitamin A and B 2 for various third order phase parameters. Moreover, selective excitation is observed for retinal versus retinol, although they differ only in a side chain. The presented approach of guiding shaped pulses through kagome fibers has large perspectives for endoscopic applications.

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Section: Laser Physicsmentioning

confidence: 99%

Contrast improvement of autofluorescent vitamins by using shaped laser pulses after a kagome fiber

Weber¹,

Otto²,

Lindinger³

2019

Laser Phys.

Self Cite

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“…In the present contribution, the phases of perpendicularly polarized subpulses are optimized by cubic phase functions for the selective excitation of FAD or NADH, respectively. These special antisymmetric phase functions will be employed for phase scans of the multiphoton excitation fluorescence [17].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence contrast improvement by polarization shaped laser pulses for autofluorescent biomolecules

Haas

Hild

Kussicke

et al. 2020

Optik

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We present contrast enhancement for the autofluorescing coenzymes flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NADH) in glycerol using phase and polarization shaped laser pulses after the transmission through a kagome fiber. Thereto, we report a way to calculate the optimal light modulator incident polarization angle, which in general differs from the horizontal. Combining phase and polarization shaping, we can selectively excite FAD in one polarization and simultaneously NADH in the other polarization direction by third order phase functions. Due to high anisotropy, the contrast of the fluorescence depends on the polarization direction. The effect of the fiber on the phase is precompensated in order to obtain the desired phase function after the fiber. Since the relative amounts of NADH and FAD give information about cellular metabolic activity which in turn helps understand disease processes, the method promises high biophotonic potential.

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“…The tailored laser pulses cause selective multiphoton induced fluorescence of dye mixtures. Special antisymmetric phase functions will be employed for scans of the multiphoton excitation fluorescence [12]. The application of phase and polarization tailored pulses for imaging contrast enhancement is demonstrated for dye molecules in a liquid after transmitting a nanostructured kagome fiber.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence anisotropy excitation by polarization-shaped laser pulses after transmission through a kagome fiber

et al. 2016

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We report improved fluorescence contrast between dyes by twophoton excitation with polarization shaped laser pulses after a kagome fiber utilizing the anisotropy of the dye molecules. Particularly phase and polarization tailored pulse shapes are employed for two-photon excited fluorescence of dyes in a liquid environment at the distal end of the kagome fiber. The distortions due to the optical fiber properties are precompensated in order to receive predefined polarization shaped laser pulses after the kagome fiber. This enables to optimally excite one dye in one polarization direction and simultaneously the other dye in the other polarization direction. The presented method has a high potential for endoscopic applications due to the unique properties of kagome fibers for guiding ultrashort laser pulses.

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Influence of nonlinear effects on the three-photon excitation of L-Tryptophan in water using phase-shaped pulses

Cited by 5 publications

References 22 publications

Contrast improvement of autofluorescent vitamins by using shaped laser pulses after a kagome fiber

Contrast improvement of autofluorescent vitamins by using shaped laser pulses after a kagome fiber

Fluorescence contrast improvement by polarization shaped laser pulses for autofluorescent biomolecules

Fluorescence anisotropy excitation by polarization-shaped laser pulses after transmission through a kagome fiber

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